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For Protein Analysis and Basic Study of Recombinant Antibody
Fc fusion vectors

pCAG is a gene expression vector for mammalian cells. pCAG vector includes CAG promoter in itself. CAG promoter is known to be able to artificially induce high expression of target recombinant proteins in mammalian cells. pCAG-Hyg/Neo IgG-Fc contains each IgG Fc region including hinge sequence at C-terminal part of multiple cloning site (MCS) and expresses Fcfused proteins. Additionally, secretory signal is inserted into N-terminal part of MCS, so that Fc-fused chimeric proteins are secreted in a culture media *1.
Fc-fused chimeric proteins have functional properties derived from the original protein, and also have features such as a long half-life, increased expression of chimeric proteins in mammalian cell, and easy purification by protein A or G.
Thus, Fc-fused chimeric proteins have been used for functional analysis of proteins and basic study of recombinant antibody.


1. LINK

2. Features

  • High expression by CAG promoter
  • Optimized expression level of drug resistance gene
  • Secretory signal is already inserted into N-terminal part of MCS
  • Many restriction enzymes can be used in MCS
  • License free *2

3. Vector Map


*1: There is a possibility that Fc-fused chimeric proteins is not secreted in a conditioned medium. It depends on characters of a target protein.
*2: Wako Products (the Products) are intended for research use only. If you have required for sales and/or development of new vector which were constructed by DNA fragments and/or sequence information of the Products, please contact us (cservice@wako-chem.co.jp)

4. Restriction Enzyme List

Vector Acc I BamH I Cla I EcoR I EcoR I Hind III Pst I Sal I Sma I Spe I Xho I
pCAG-Hyg hIgG1-Fc
 
   
 
pCAG-Hyg mIgG1-Fc  
 
   
 
pCAG-Hyg mIgG2a-Fc
 
   
 
pCAG-Hyg mIgG2b-Fc  
 
   
 
pCAG-Hyg rIgG2a-Fc  
 
   
 
pCAG-Hyg rIgG2b-Fc  
 
   
 
pCAG-Neo hIgG1-Fc
 
pCAG-Neo mIgG1-Fc  
 
pCAG-Neo mIgG2a-Fc
pCAG-Neo mIgG2b-Fc  
pCAG-Neo rIgG2a-Fc  
pCAG-Neo rIgG2b-Fc  

1)   Mean particle size is a middle one in the accumulated volume distribution.
2)   5 % Dynamic Binding Capacity is measured by frontal analysis (Residence time 3 minutes).

5. Product List

Selection Marker Product Name Pkg. Size Wako Cat. No. Storage Condition
Selection Marker Mammalian Cell
Hygromycin B     pCAG-Hyg hIgG1-Fc
      ⇒ Sequence
20 μg 165-27741
Keep at -20°C.
    pCAG-Hyg mIgG1-Fc
      ⇒ Sequence
20 μg 162-27751
Keep at -20°C.
    pCAG-Hyg mIgG2a-Fc
      ⇒ Sequence
20 μg 169-27761
Keep at -20°C.
    pCAG-Hyg mIgG2b-Fc
      ⇒ Sequence
20 μg 166-27771
Keep at -20°C.
    pCAG-Hyg rIgG2a-Fc
      ⇒ Sequence
20 μg 163-27781
Keep at -20°C.
    pCAG-Hyg rIgG2b-Fc
      ⇒ Sequence
20 μg 160-27791
Keep at -20°C.
Kanamycin G418     pCAG-Neo hIgG1-Fc
      ⇒ Sequence
20 μg 165-27741
Keep at -20°C.
    pCAG-Neo mIgG1-Fc
      ⇒ Sequence
20 μg 162-27751
Keep at -20°C.
    pCAG-Neo mIgG2a-Fc
      ⇒ Sequence
20 μg 169-27761
Keep at -20°C.
    pCAG-Neo mIgG2b-Fc
      ⇒ Sequence
20 μg 166-27771
Keep at -20°C.
    pCAG-Neo rIgG2a-Fc
      ⇒ Sequence
20 μg 163-27781
Keep at -20°C.
    pCAG-neo rIgG2b-Fc
      ⇒ Sequence
20 μg 160-27791
Keep at -20°C.

6. Related Product

Product Name Pkg. Size Wako Cat. No. Storage Condition
    KANEKA KanCapA™
            ⇒ Package Insert
2 mL (net 1 mL) 114-01071
Keep at 2-10°C.
10 mL (net 5 mL) 110-01073
50 mL (net 25 mL) 118-01074
KANEKA KanCapA™ is an affinity chromatography resin designed for the purification of biomolecules containing an Fc region of immunoglobulin and its derivatives derived from biological samples and cell culture media. Please check the leaflet.
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