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Reagent for assisting gene transfer
GoldMAN™

GoldMAN™ is a magnetic gold composite nanoparticle solution consisting of magnetic iron oxide nanoparticles carrying numerous gold colloids a few nanometers in size.   It can be used for transfer of DNA, RNA, oligonucleotides, siRNA, etc. into cells.
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⇒ 1. Features
⇒ 2. Product Outline
⇒ 3. Outline of Gene Transfer
⇒ 4. Application
⇒ 5. References
⇒ 6. Product List



1. Features

(1) Direct sensitization of virus vectors to the gold region
(2) High efficiency of gene transfer into cells without a virus receptor
(3) Absence of cytotoxicity and applicability to various cells
(4) Applicable to magnetic separation and purification of gene-expressed cells


2. Product Outline


3. Outline of Gene Transfer


4. Application

Cells Increase in gene expression*1 CAR*2 expression level
B16BL6 (mouse melanoma cell line) 3,000 folds Low
NIH3T3 (mouse embryo fibroblast cell line) 800 folds Low
Colon26 (mouse colon cancer cell line) 600 folds Middle
A549 (human lung adenocarcinoma cell line) 15 folds High
*1 The gene expression when adenovirus vector carrying luciferase gene and GoldMANTM were used was evaluated by the luciferase assay.
*2 CAR: Coxsackievirus and Adenovirus Receptor: A receptor required for cellular penetration of adenovirus.



5. References
  1. "Magnetic Composite Nanoparticles of Au/γ-Fe2O3 Synthesized by g-ray Irradiation" S. Seino, T. Kinoshita, Y. Otome, K. Okitsu, T. Nakagawa and T. A. Yamamoto, Chemistry Letters, 32 (2003) 690-691.
  2. "Magnetic Separation of Amino Acids by Gold/Iron-Oxide Composite Nanoparticles Synthesized by Gamma-ray", T. Kinoshita, S. Seino, Y. Otome, Y. Mizukoshi, T. Nakagawa, K. Okitsu and T. A. Yamamoto, J. Magn. Magn. Mater., 293 (2005) 106-110.
  3. "DNA Separation Using Gold/Magnetic Iron-oxide Composite Nanoparticles" T. Kinoshita, S. Seino, Y., Otome, Y. Mizukoshi, T. Nakagawa, T. Nakayama, T. Sekino, K. Niihara and Takao. A. Yamamoto. Mat., Res. Soc. Symp. Proc., 877E (2005) S6.30.1-6.
  4. "Radiation Induced Synthesis of Gold/Iron-oxide Composite Nanoparticles Using High Energy Electron Beam" S. Seino, T. Kinoshita, T. Nakagawa, T. Kojima, R. Taniguchi, S. Okuda and T. A. Yamamoto, Journal of Nanoparticle Research, 10 (2008) 1071-1076.
  5. "Direct cell-entry of gold/iron-oxide magnetic nanoparticles in adenovirus mediated gene delivery", Kamei K, Mukai Y, Kojima H, Yoshikawa T, Yoshikawa M, Kiyohara G, Yamamoto TA, Yoshioka Y, Okada N, Seino S, Nakagawa S., Biomaterials 30 (2009) 1809-1814.
  6. "Simple PEG conjugation of SPIO via an Au-S bond improves its tumor targeting potency as a novel MR tumor imaging agent", Kojima, H., Mukai, Y., Yoshikawa, M., Kamei, K., Yoshikawa, T., Morita,M., Inubushi, T., Yamamoto, T.A., Yoshioka, Y., Okada, N., Seino, S.,Nakagawa, S. Bioconjugate Chemistry, 21(6)�@(2010)pp. 1026-1031.



LINK
ACT NONPAREIL Co., Ltd. (Osaka, Japan)
The company utilizes magnetic gold nanoparticles and offers magnetic beads meeting environments and purposes of use.
Click below for details of respective products.
ACT NONPAREIL homepage


6. Product List
Product name Package Size Wako Catalog Number
GoldMAN™ 2 mL 304-94621
Products listed in this page are for research use only.   Do not administer each to human.
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