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Primary Antibody Detection from various animal species
Multi Capture HRP

This reagent can detect almost all primary antibodies regardless of the immunized animal species where it is mouse, rat, rabbit, or hamster.
This means you can perform western blotting without preparing labeled secondary antibodies against the animal species in which the primary antibody was raised.

INDEX

  1. Features

  2. Kit Components

  3. Example of use

  4. Product List

  5. Related Products

  6. Link to the manual (321 KB / 4P)
    Link to a brochure Proteomics Research 2012

Multi Capture HRP



1. Features
  • It can be used to detect primary antibodies raised in a variety of species.
    Note: It is less suitable for detection of mouse IgG1 and goat IgG.
  • Can simultaneously detect multiple antigens.
  • Signals detected by a secondary antibody can be enhanced
  • Low lot-to-lot variability.


2. Kit Components
  291-71801 (10 reactions) 297-71803 (40 reactions)
(1)   Capture Reagent 50 μL 200 μL
(2)   20 x Reaction Buffer 10 mL 40 mL
(3)   Enhancer Reagent for Mouse 50 μL 200 μL
*Package size is based on use when the capture reagent is diluted to 2,000-fold. Each size can be used for 50 and 200 reactions when the capture reagent is diluted to 10,000-fold.
*Use the enhancer reagent to enhance signals detected when the target animal species is mouse.



3. Example of use
Comparison with a secondary antibody

[Sample]
THP-1 cell lysate + DYKDDDDK fusion protein

[Primary antibody]
Anti-DYKDDDDK tagged, rabbit,
Polyclonal antibody (0.5 μg/mL)
[Secondary antibody]
HRP-labeled anti-rabbit IgG polyclonal antibody
Detection of multiple antigens

[Primary antibody]
Anti-GFP, rabbit polyclonal antibody
(x 2,000)
Anti-GAPDH, rabbit monoclonal antibody
(x10,000)
Enhancement of signals after reaction with secondary antibody

[Sample]
HuH7 cell lysate
[Primary antibody]
Anti-Vimentin, mouse IgG2a (x 1,000)
[Secondary antibody]
HRP-labeled anti-mouse IgG polyclonal antibody
(x 5,000)
Enhanced detection of mouse IgG1 antibody

[Sample]
THP-1 cell lysate + DYKDDDDK fusion protein
[Primary antibody]
Anti-DYKDDDDK, mouse IgG1 monoclonal antibody (1 μg/mL)
[Secondary antibody]
HRP-labeled mouse IgG polyclonal antibody (diluted to 5,000-fold)
(Code No. 018-23643)



4. Product List
Product Name Grade Package Size Wako Catalog No.
Multi Capture HRP for Blotting 10 reactions 291-71801
40 reactions 297-71803



5. Related Products
Product Name Grade Package Size Wako Catalog No.
ImmunoStar® LD for Blotting 200 cm2 296-69901
1,000 cm2 292-69903
2,000 cm2 290-69904
Anti-mouse IgG (H + L), rabbit, IgG fraction,
Peroxidase conjugate
for Immunochemistry 300 μL 012-23641
1 mL 018-23643
QUOTATION REQUEST for reagents for Multi Capture HRP
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Note

  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
  • Please visit our online catalog to search for other products from Wako: http://www.e-reagent.com
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