This website uses cookies. By using the site you are agreeing to our Privacy Policy.

FUJIFILMFUJIFILM Wako Pure Chemical Corporation Laboratory Chemicals
Home  > Products >  Life Science > Disease Research  >  High Molecular Amyloid β Oligomer ELISA Kit Wako

For Alzheimer’s disease
High Molecular Amyloid β Oligomer ELISA Kit Wako

Recently, amyloid β(Aβ) oligomer has focused as a cause of Alzheimer's disease. Aβ oligomer is a soluble aggregate by polymerized of Aβ monomers, and it impairs synaptic function of neurons. Aβ oligomer has several types, low molecular Aβ oligomers(Low-n oligomer) and high molecular Aβ oligomers (ADDL, Aβ*56 and proto-fibril). It has reported that Aβ * 56 (12-mer) oligomer greatly affects memory damage1), and in cognitively normal adults Aβ*56 increased ahead of amyloid-β dimers or amyloid-β trimers, and pathological tau proteins and postsynaptic proteins correlated with Aβ*56, but not amyloid-β dimers or amyloid-β trimers2).
This item is an ELISA kit that can specifically measure high molecular Aβ oligomers.

[Reference]
1) Lesné, S., et al.: Nature, 440, 7082, 352(2006).
2) Lesné, S. E., et al.: Brain, 136, 1383(2013).

 

1. Links

2. Features

Specifically reactive with ≧9mer Aβ oligomer

3. Kit components

1 Antibody (BAN50)-coated Plate 1 Plate
2 Standard Solution (10 nM) 100 μL
3 Buffer 60 mL
4 Biotin-conjugated Antibody(BAN50) Solution 100 μL
5 Peroxidase-conjugated Streptavidin Solution 100 μL
6 Luminescent Reagent 1 6 mL
7 Luminescent Reagent 2 6 mL
8 Wash Solution (10 x) 100 mL
9 Plate Seal 3 sheets

4. Performance

Standard curve range 0.410~100 pM*
Reactive Aβ oligomers ≧9mer Aβ oligomer
Sample** Cerebrospinal fluid
In vitro Aβ oligomer
Cross-reactivity Human
Sample volume 25μL(4 times dilution)
Assay time 4.5 hours
Detection method Luminescence method***

 

   *Calculated on the basis of 16-mer MAP peptide.
   ** Plasma and serum are not recommended.
   ***A light-emitting plate reader is required for measurement.

 

5. Assay principal

The sandwich ELISA using the same Aβ antibody(BAN50) for both capture and detection specifically reacts with ≧9mer Aβ oligomer, not react with 1-8mer Aβ3)4). As a standard, a 16-mer MAP peptide with 16 epitopes of anti Aβ antibody (BAN50) is used and the measured value is the value calculated on the basis of 16-mer MAP peptide4).

○Standard (16-mer MAP peptide)





3) Fukumoto, H., et al.: FASEB J., 24, 2716(2010).
4)Kasai, T., et al.: Biochem. Biophys. Res. Commun., 422, 3, 375(2012).

6. Specificity for Aβ oligomers in the ELISA

Aβ monomers were incubated the solution at 4℃ overnight in order to synthesize various size of Aβ oligomers. They were separated Fraction A and Fraction B by ultrafiltration. The fractions were analyzed by western blotting and the Aβ oligomer ELISA.

■Western blotting using Aβ antibody(BAN50)
■Aβ oligomer ELISA
A strong signal was exhibited in the fraction A containing 9-mer and more (>30 kDa),
whereas few signal was detected in fraction B containing 8-mer and smaller (<30 kDa).

7. Example of measurement – human cerebrospinal fluid-

Cerebrospinal fluids of patients with Alzheimer disease (AD) and non-dementia patients(control), were measured with our ELISA kit and competitor’s kit.

Wako
Company A

 

MMSE scoreWakoCompany A
Aβ oligomer kit (pM)Aβ oligomer kit (pg/mL)
Control 1- 0.98ND
Control 2- 1.9933
Control 3- 1.6937
Control 4- 4.5988
Control 5- 1.7355
AD1204.490
AD2204.11113
AD3264.95100
AD4245.8478
AD5155.74105
**** MMSE score:diagnosis Inquiry test score of cognitive function
30-28 Normal
27-24 Suspected of mild cognitive impairment
≦23 Suspected of dementia

 

→There was a difference between non-dementia patients and Alzheimer's disease,
and the difference of Wako’s item is larger than competitor’s.

8. Product list

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
High Molecular Amyloid β Oligomer ELISA Kit Wako 96 tests

298-80101

For Immunochemistry Keep at 2-10℃

※Not available for sale in Europe.

9. Related product lists

Aβ ELISA kit

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Human β Amyloid(1-40) ELISA Kit Wako 96 tests 292-62301 For ImmunochemistryKeep at 2-10℃
Human β Amyloid(1-40)ELISA Kit Wako96 tests 298-64601 For ImmunochemistryKeep at 2-10℃
Human/Rat β Amyloid(40)ELISA Kit Wako 96 tests 294-62501 For ImmunochemistryKeep at 2-10℃
Human/Rat βAmyloid(40)ELISA Kit Wako96 tests 294-64701 For ImmunochemistryKeep at 2-10℃
Human β Amyloid(1-42) ELISA Kit Wako96 tests 298-62401 For ImmunochemistryKeep at 2-10℃
Human β Amyloid(1-42) ELISA Kit Wako, High Sensitive 96 tests 296-64401 For ImmunochemistryKeep at 2-10℃
Human/Rat β Amyloid(42)ELISA Kit Wako96 tests 290-62601 For ImmunochemistryKeep at 2-10℃
Human/Rat β Amyloid(42) ELISA Kit Wako, High Sensitive 96 tests 292-64501 For ImmunochemistryKeep at 2-10℃
Total Tau ELISA kit

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Tau ELISA Kit Wako 96 tests

296-80401

For Immunochemistry Keep at 2-10°C
Aβ antibodies

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Anti Human Amyloid β, Monoclonal Antibody (BAN50) 10 μL 017-26871 For ImmunochemistryKeep at -20℃
50 μL 013-26873 For ImmunochemistryKeep at -20℃
Anti Amyloid β40, Monoclonal Antibody (BA27) 10 μL 018-26921 For ImmunochemistryKeep at -20℃
50 μL 014-26923 For ImmunochemistryKeep at -20℃
Anti Amyloid β42(43), Monoclonal Antibody (BC05) 10 μL 014-26901 For ImmunochemistryKeep at -20℃
50 μL 010-26903 For ImmunochemistryKeep at -20℃
Anti Amyloid β, Monoclonal Antibody (BNT77) 10 μL 014-26881 For ImmunochemistryKeep at -20℃
50 μL 010-26883 For ImmunochemistryKeep at -20℃
Anti Amyloid Precursor Protein, Monoclonal Antibody (3E9) 10 μL 014-27241 For ImmunochemistryKeep at -20℃
50 μL 010-27243 For ImmunochemistryKeep at -20℃
Home  > Products >  Life Science > Disease Research  >  High Molecular Amyloid β Oligomer ELISA Kit Wako