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for Brain Tumor and Cancer Research
Anti IDH, Monoclonal Antibodies

Isocitrate dehydrogenase (IDH) is an oxidoreductase that interconverts isocitric acid and α-ketoglutarate. There are IDH1 (cytoplasm, NADP+ dependent), IDH2 (mitochondria, NADAP+ dependent), and IDH3 (mitochondria, NAD+ dependent) isozymes known in mammals.
Glioma is classified by WHO as shown in Table 1. Its malignancy varies from benign to malignant glioblastoma. Frequent mutations of IDH1 and IHD2 have been reported in WHO Grade II/III glioma or grade IV glioblastoma developed from low-grade glioma, demonstrating their association with glioma. This series of monoclonal antibodies specifically recognize IDH1, IDH2, and IDH1-R132H and IDH1-R132S mutations.

INDEX

  1. Anti IDH1, Monoclonal Antibody
  2. Anti IDH2, Monoclonal Antibody
  3. Anti IDH1-R132H, Monoclonal Antibody
  4. Anti IDH1-R132S, Monoclonal Antibody

  5. References

  6. Product List
  7. Related Products


1. Anti IDH1, Monoclonal Antibody
<Product overview>
Appearance PBS solution containing 0.05% sodium azide (pH: 7.2)
Antigen wild type IDH1, mutated IDH1
Cross Reactivity Human, mouse, and hamster
Immunized Animal Mouse
Clone No. RMab-3
Subclass IgG1
Working Dilution ELISA: 1-5 μg/mL
Western blot: 5 μg/mL
Immunohistochemistry: 5-10 μg/mL

2. Anti IDH2, Monoclonal Antibody

<Product overview>
Appearance PBS solution containing 0.05% sodium azide (pH: 7.2)
Antigen wild type IDH2, mutated IDH2
Cross reactivity Human, mouse, and hamster
Immunized Animal Mouse
Clone No. RMab-22
Subclass IgG2b
Working Dilution ELISA: 1-5μg/mL
Western blot: 5μg/mL
Immunohistochemistry: 5-10μg/mL
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3. Anti IDH1-R132H, Monoclonal Antibody

<Product overview>
Appearance PBS solution containing 0.05% sodium azide (pH: 7.2)
Antigen IDH1-R132H
Cross Reactivity -
Immunized Animal Mouse
Clone No. HMab-1
Subclass IgG1
Working Dilution ELISA: 1-5μg/mL
Western blot: 5μg/mL
Immunohistochemistry: 5-10μg/mL

4. Anti IDH1-R132S, Monoclonal Antibody

<Product overview>
Appearance PBS solution containing 0.05% sodium azide (pH: 7.2)
Antigen IDH1-R132S
Cross Reactivity -
Immunized Animal Mouse
Clone No. SMab-1
Subclass IgG1
Working Dilution ELISA: 1-5μg/mL
Western blot: 5μg/mL
Immunohistochemistry: 5-10μg/mL
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5. References
  1. Kato, Y. et al.: "GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice", Biochem. Biophys. Res. Commun., 391, 750-755 (2010).
  2. Kato, Y. et al.: "A monoclonal antibody IMab-1 specifically recognizes IDH1R132H, the most common glioma-derived mutation", Biochem. Biophys. Res. Commun., 390, 547-551 (2009).
  3. Takano, S. et al.: "Detection of IDH1 mutation in human gliomas: comparison of immunohistochemistry and sequencing", Brain Tumor Pathol., 28, 115-123 (2011).
  4. Kaneko, M. K. et al.: "Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation", Biochem. Biophys. Res. Commun., 406, 608-613 (2011).
  5. Kato. Y. et al.: "Inhibition of tumor cell-induced platelet aggregation using a novel anti-podoplanin antibody reacting with its platelet aggregation-stimulating domain", Biochem. Biophys. Res. Commun., 349, 1301-1307(2006).

6. Product List
Product Name Grade Pkg. Size Wako Cat. No. Storage Conditions
Anti IDH1, Monoclonal Antibody for Immunochemistry 100 μg 014-24061 Keep at -20°C
Anti IDH2, Monoclonal Antibody for Immunochemistry 100 μg 011-24071 Keep at -20°C
Anti IDH1-R132H, Monoclonal Antibody for Immunochemistry 100 μg 018-24081 Keep at -20°C
Anti IDH1-R132S, Monoclonal Antibody for Immunochemistry 100 μg 015-24091 Keep at -20°C
QUOTATION REQUEST for Anti IDH

7. Related Products
Product Name Grade Pkg. Size Wako Cat. No. Storage Conditions
Anti Human Podoplanin, Monoclonal Antibody for Immunochemistry 100 μg 018-24101 Keep at -20°C
Anti Mouse Podoplanin, Monoclonal Antibody for Immunochemistry 100 μg 015-24111 Keep at -20°C
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