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A microglial marker
Anti Iba1 antibody series

Iba1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage/microglia. Recently, microglia has attracted attentions because neurological damage effect by production of NO, TNF-α, and IL-1β have been proved in addition to its role in neurotrophic effects/neuroprotective actions. These products are antibodies that specifically recognize Iba1, and are available for a microglial marker.

 

1. Link

2. Product Type

TypeProduct NameWako Cat. NoPkg. SizeCross-reactivityApplications
Rabbit polyclonal
antibody
Anti Iba1, Rabbit (for Immunocytochemistry) 019-1974150μgMouse, rat, humanIHC(F),ICC
Anti Iba1, Rabbit(for Paraffin Section) 013-2769150μgMouse, ratIHC(P)
Anti Iba1, Rabbit, Biotin-conjugated 016-26461100μLMouse, ratIHC(F)
Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated013-26471100μLMouse, ratIHC(F)
Anti Iba1, Rabbit (for Western Blotting) 016-2000150μgMouse, rat, humanWB
Mouse monoclonal antibodyAnti Iba1, Monoclonal Antibody
(NCNP24)
012-2672310μLMouse, rat, marmosetIHC(F)
016-2672150μL
Anti Human Iba1, Monoclonal Antibody
(NCNP27)
017-2759110μLHumanIHC(P)
013-2759350μL

IHC(F):immunohistochemistry(frozen section) IHC(P):immunohistochemistry(paraffin section) ICC:immunocytochemistry WB:western blotting

■Experimental condition
Code No. 019-19741/012-26723/016-26721
Tissue:rat brain cortex
Section:frozen section
Antibody concentration:1/1,000

Code No.016-26461/013-26471
Tissue:rat brain cortex
Section:frozen section
Antibody concentration:1/200

 

These data were provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

3. Antibody Information

Polyclonal antibodyMonoclonal antibody
 ProductAnti Iba1,
Rabbit
(for Immuno-
cytochemistry)
Anti Iba1,
Rabbit
(for Paraffin
Section)
Anti Iba1,
Rabbit,
Biotin-
conjugated
Anti Iba1,
Rabbit,
Red Fluorochrome
(635)-
conjugated
Anti Iba1,
Rabbit
(for Western
Blotting)
Anti Iba1,
Monoclonal
Antibody
(NCNP24)
Anti Human
Iba1,
Monoclonal
Antibody
(NCNP27) 
Wako No.
(Pkg. Size)
019-19741
(50 μg)
013-27691
(50μg)
016-26461
(100 μL)
013-26471
(100 μL)
016-20001
(50 μg)
012-26723
(10 μL)
017-27591
(10 μL)
016-26721
(50 μL)
013-27593
(50 μL)
AntigenSynthetic peptide
(C-terminal of Iba1)
Synthetic peptide
(C-terminal of Iba1)
Clone No.- (Polyclonal) NCNP24NCNP27
SubclassRabbit IgGMouse IgG1Mouse IgG2
Conjugate--BiotinRed Fluorochrome
(Ex=634, Em=654 nm
---
Concent-
ration
0.5 mg/mL0.5 mg/mL0.5 mg/mL0.5 mg/mL0.5 mg/mL1.3 mg/mL
(First lot)
1.0 mg/mL
(First lot)
BufferTBSTBSPBS,0.05% NaN3PBS,0.05% NaN3TBS50% Glycerol
/TBS,
0.05% NaN3
50% Glycerol
/TBS,
0.05%NaN3
Cross- reactivityMouse, rat, human etc*Mouse, ratMouse, ratMouse, ratMouse, rat, humanMouse, rat, marmoset Human
Applications■Immunohisto-
chemistry
(frozen section)
1:500-1,000

■Immunocyto-
chemistry
1:500-1,000
■Immunohisto-
chemistry
(paraffin section)
1:500-1,000
■Immunohistochemistry
(frozen section)
1:200-2,000
■Western
Blotting
1:500-1,000
■Immuno-
histochemistry
(frozen section,
DAB)
1:500-2,000

■Immuno-
histochemistry
(frozen section,
fluorescent)
1:100
■Immuno-
histochemistry
(paraffin section,
DAB)
1:100-1,000
Reference1)2)3)4)5)-6)-7)8)9)10)-

* The paper using samples of dog1), cat2), pig3),marmoset4) and zebrafish5) have reported.

 

■Reference

1) Ahn, J.H., et al.: Lab. Anim. Res., 28, 3, 165 (2012).
2) Ide, T., et al.: J. Vet. Med .Sci., 72, 1, 99 (2010).
3) Gaigé, S., et al.: Neurotoxicology., 34, 135(2013).
4) Rodriguez-Callejas, J.D. et al.: Front. Aging Neurosci., 8, 315(2016).
5) Fantin, A., et al.: Blood, 116, 5, 829 (2010).
6) Jones, M. E., et al.: Brain Behav. Immun., 67, 355(2018).
7) Sun, J.S. et al.: Mol. Med. Rep., 12, 2, 2677(2015).
8) Grishchuk, Y., et al.: Am. J. Pathol., 186, 1, 199(2016).
9) Wan, S., et al.: J. Neuroinflammation, 15, 31(2018).
10) Chen, Y., et al.: Annals of Clinical and Translational Neurology,10.1002/acn3.513 (2017).

4. Select Flowerchart

5. Application Data -western blotting-

016-20001  Anti Iba1, Rabbit (for Western Blotting)

Lane
1.Iba1 (10ng)
2.Rat microglia (10 μg)
3.Rat neuron (10 μg)
4.Rat cortex (150 μg)
■Experimental condition
SDS-PAGE: 5.5 % stacking gel, 12.5 % running gel, 100V
Blocking: 3 % skim milk in TBS at RT for 1h
Primary antibodies: anti-Iba1 antibody(1/1000) in 3 % skim milk in TTBS at 4 °C O/N
Secondary antibody: peroxidase labeled anti-rabbit antibody (1/5000) in 3 % skim milk in TTBS at RT for 1h

 

The data was provided by Sanagi,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

6. Application Data –immunohistochemistry-

016-27691  Anti Iba1, Rabbit(for Paraffin Section)

Rat Mouse
■Experimental condition
Tissue:mouse and rat hippocampus neighborhood
Section:paraffin section
Antibody concentration:1/1,000

 

7. Product Lists

Product Name Wako Cat. No. Pkg. Size Grade Storage Condition
Anti Iba1, Rabbit (for Immunocytochemistry) 019-1974150μgFor Immunochemistry Keep at -20°C
Anti Iba1, Rabbit (for Paraffin Section) 013-2769150μgFor Immunochemistry Keep at -20°C
Anti Iba1, Rabbit, Biotin-conjugated 016-26461100μLFor Immunochemistry Keep at 2-10°C
Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated013-26471100μLFor Immunochemistry Keep at 2-10°C
Anti Iba1, Rabbit (for Western Blotting) 016-2000150μgFor Immunochemistry Keep at -20°C
Anti Iba1, Monoclonal Antibody (NCNP24)012-2672310μLFor Immunochemistry Keep at -20°C
016-2672150μLFor Immunochemistry Keep at -20°C
Anti Human Iba1, Monoclonal Antibody (NCNP27)017-2759110μLFor Immunochemistry Keep at -20°C
013-2759350μLImmunochemistry Keep at -20°C
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