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A prepacked new standard resin column for antibody purification
KANEKA KanCapA™ Prepacked Column

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KANEKA KanCapA™ Prepacked Column is a pre-packed column with KANEKA KanCapA™ for purification of immunoglobulins and Fc-chimeric protein from biological samples and cell culture media. KANEKA KanCapA™ is composed of highly cross-linked cellulose beads and alkaline resistant protein A ligand. It can be used for screening work, initial stage process development, and small scale purification.


1. Link

2. Features

  • Alkaline Resistance
  • Long Life Cycle
  • Optimized Elution pH
  • High Binding Capacity
  • Easy Scale Up
  • Recyclable
  • Ready to use

3. Recombinant protein A

改変型プロテインA

                        Protein A adopted in the product:

4. Product Characteristics

 Column Type (Wako Cat. No.)     1 mL (Wako Cat. No. 111-01081)     5 mL       (Wako Cat. No. 117-01083)
    5 mL x 5  (Wako Cat. No. 115-01084)
   Inner Diameter x Length     8.0 mm x 20 mm     8.0 mm x 100 mm
   Column Cross Scetion     0.5 cm2     0.5 cm2
  Column Volume     1.0 mL     1.0 mL
  Recommended Flow Rate 1)    0.17-0.25 / 20-30 (mL/min/cm/h)    0.84-1.26 / 100-150 (mL/min/cm/h)
    Max Flow Rate     0.84 / 100 (mL/min/cm/h)     4.19 / 500 (mL/min/cm/h)
    Outer Dimensions 2)     10 mm x 84 mm    

10 mm x 164 mm

 

 

    Connectors     Standard HPLC (10~32 for 1/16 inch capillaries)
    Maximum Operating Pressure     30 bar     30 bar
    Chemical Stability 3)     Columns are tolerant to solutions as follows:
  --------------------------------------------------------------------------------------------------------------
    Aqueous buffers, salt solutions, 1M alkaline solutions, 1M non-oxidizing mineral acids,
    8M urea, 6M guanidine hydrochloride, non-halogenated organic solvents and detergents
  ---------------------------------------------------------------------------------------------------------------
    They are not compatible with strong oxidants and halogenated solvents.
    Storage Condition 4)     20% Ethanol suspension at 2 ~ 10°C

1)   Recommended residence time: 4~6 minutes
2)   Including stoppers
3)   The chemical stability refers to the column hardware parts only. For the chemical stability of KANEKA KanCapA™, please see the "Table 1: Physical and chemical properties of KANEKA KanCapA™" of the package insert.
4)   Prevent from freezing.

5. Product List

Product Name Package Size Wako Catalog No. Grade Storage Condition
    KANEKA KanCapA™ Prepacked Column
1 mL
5 mL
5 mL x 5
111-01081
117-01083
115-01084
for Genetic Research Keep at 2-10°C.

6. Related Product

KANEKA KanCapA™ - A new standard resin for antibody purification -
KANEKA KanCapA™ is an affinity chromatography resin immobilized Protein A. It was designed for the purification of biomolecules containing Fc region of immunoglobulin and its derivatives derived from biological samples and cell culture media. KANEKA KanCapA™ is composed of highly cross-linked cellulose beads and Alkaline Resistant Protein A ligand. It can be used for affinity purification of IgG.
Product Name Package Size Wako Catalog No. Grade Storage Condition
        KANEKA KanCapA™

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  2 mL   (net  1 mL)
10 mL   (net  5 mL)
50 mL   (net 25 mL)
114-01071
110-01073
118-01074
for Genetic Research Keep at 2-10°C.
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