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Home > Products > Life Science > L-Alanyl-L-glutamine solution: an Alternative to L-glutamine in Cell Culture

for Cell Culture
200 mmol/L L-Alanyl-L-Glutamine Solution, an Alternative to L-Glutamine in Cell Cultures




1. Features
  • Improvement of Stability of Media
  • Inhibition of Ammoniagenesis harmful to cells
  • Improvement of Cell Proliferation Potency



2. Application:
Culture of CHO cells in a medium containing L-Ala-L-Gln

Medium: D-MEM (high glucose) without L-glutamine (#045-30285)
                + 10% FCS
                + 2 mmol/L L-alanyl-L-glutamine
                       (L-glutamine (Wako Cat. #073-05391) of the same concentration was added to the control)

      Culture Results
      Advantage of Addition of L-Ala-L-Gln to a medium
  • Allows cell culture for a long period of time (6 days) (On the other hand, allows only 4 days in the medium containing L-Gln)
  • Allows cell culture at a high density (approximately 1.25 times the density in the medium containing L-Gln).
      Usage
          Addition to a medium not containing L-glutamine.



3. Product List
Product Name Specification Package Size  (Wako Catalog No.)
200mmol/L L-Alanyl-L-Glutamine Solution (x 100) for Cell Culture 100 mL  (#016-21841)

Media not containing L-Glutamine
Product Name L-Gln Phenol Red Pyr-Na HEPES Package Size (Wako Catalog No.)
D-MEM (High Glucose) - - - 500 mL  (#045-30285)
- - - - 500 mL  (#040-30095)
RPMI-1640 - - - 500 mL  (#183-02165)

Antibiotic Solutions and Medium Additive Solution
Product Name Specification Package Size    (Wako Catalog No.)
Penicillin-Streptomycin Solution (x 100) for Cell Culture 100 mL  (#168-23191)
Penicillin-Streptomycin-Amphotericin B Suspension (x 100) for Cell Culture 100 mL  (#161-23181)
Penicillin-Streptomycin-L-Glutamine Solution (x 100) for Cell Culture 100 mL  (#161-23201)
G-418 Sulfate Solution for Genetic Research 20 mL  (#076-05381)
100 mL  (#072-05383)
50 mg/mL Hygromycin B Solution for Biochemistry 20 mL  (#084-07681)
100  mL (#080-07683)
50 mg/mL Gentamicin Solution for Cell Culture 10 mL  (#072-05481)
200 mmol/L L-Glutamine Solution (x 100) for Cell Culture 100 mL  (#073-05391)
100 mmol/L Sodium Pyruvate Solution (x 100) for Cell Culture 100 mL  (#190-14881)
MEM Non-essential Amino Acids Solution (x 100) for Cell Culture 100 mL  (#139-15651)
MEM Essential Amino Acids Solution (x 50) for Cell Culture 100 mL  (#132-15641)
Products listed in this page are for research use only.  Do not administer each to human.



Home > Products > Life Science > L-Alanyl-L-glutamine solution: an Alternative to L-glutamine in Cell Culture