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Biochemical Test kits
LabAssay™ Series

The LabAssay™ Series is a collection of biochemical assay kits for analyzing samples from humans, mice, and other animals. Since analyses are performed in microtiter plates, many samples can be concurrently evaluated, and only small sample amounts are needed.
※For Research Use Only, Not for Diagnostic Use

1. LabAssay™ Ammonia

Ammonia is mainly produced in the intestine or the kidney. Ammonia produced in the body is converted to urea through a series of reactions known as the urea cycle in the liver and eliminated through urine.

Assay Principle (Modified Fujii-Okuda Method)

Ammonia is converted to Dioxydiphenylamine by the reaction of Phenol and Sodium Pentacyanonitrosyl ferrate(III). A reaction of the Dioxydiphenylamine with Sodium Hypochlorite produces Indophenol ,which pigments blue, under alkaline conditions. LabAssay ™ Ammonia is a kit used for the quantitative determination of ammonia nitrogen in samples by measuring absorbance of the blue color.

Kit Contents                     Standard Curve

1

Deproteinizing

100mL×1vial

2

Chromogen Reagent A

50mL×1vial

3

Chromogen Reagent B

25mL×1vial

4

Chromogen Reagent C

50mL×1vial

5

Ammonia Standard Solution

15mL×1vial

6

Dilute olution for Standard

20mL×1vial

Performance

Standard curve range 100~400μg/dL(μg/100mL)
Measurement time approx. 70 minutes
Amount of sample 70μL
Measurement wavelength 630nm

Product list

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™ Ammonia for Cellbiology 700 tests

295-78901

Keep at 2-10℃

※Not available for sale in India.

2. LabAssay™ ALP

Alkaline Phosphatase (ALP) is an enzyme distributed in a variety of tissues such as the liver, bone and small intestines in animals. Especially, it is used one of the Osteogenesis Markers in bone metabolism research area.

Assay Principle (Alkaline Phosphatase activity assay with p-Nitrophenyl Phosphate as a substrate)

p-Nitrophenylphosphate is hydrolyzed into p-Nitrophenol in the presence of Alkaline Phosphatase(ALP). LabAssay™ ALP is a kit designed to determine Alkaline Phosphatase activity in samples by measuring the amount of p-Nitrophenol released by p-Nitrophenylphosphate as a phosphatase substrate.

Kit Contents                     Standard Curve

1

Substrate Tablet

20 tablets

2

Buffer Solution

100mL×1vial

3

Stop Solution

100mL×1vial

4

Standard Solution

10mL×1vial

Performance

Standard curve range 0.0652~0.5mmol/L
Measurement time approx. 20 minutes
Amount of sample 20μL
Measurement wavelength 405nm

Reference

1) Ito, S. et al.: J. Pharmacol. Exp. Ther., 333, 341 (2010). ※Extraction liquid of mouse kidney
2) Iwakura, T. et al.: J. Orthop. Res., 27, 208 (2009). ※Extraction liquid of human culture cells
3) Furuya, Y. et al.: J. Biol. Chem., 286, 37023 (2011). ※Mouse serum
4) Itoh, T. et al.: J. Biol. Chem., 284, 19272 (2009). ※Extraction liquid of mouse culture cells

Product List

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™ ALP for Cellbiology 900 tests

291-58601

Keep at 2-10℃

3. LabAssay™ Cholesterol

Cholesterol, a major component of cell membranes and the starting material in steroid synthesis in many animals, is a factor behind arteriosclerosis and other vascular diseases.

Assay Principle (Cholesterol Oxidase・DAOS method)

Hydrogen peroxide produced by a reaction of cholesterol and cholesterol oxidase let N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS)and 4-Aminoantipyrin oxidize and condensate. LabAssay™ Cholesterol is a kit to determine total cholesterol by measuring absorbance of the blue color which is generated by the oxidative condensation reaction.

Kit Contents                     Standard Curve

1 Buffer 150mL×2vials

2

Chromogen for 150mL×2vials
3 Standard Solution 10mL×1vial

Performance

Standard curve range 50~592.2mg/dL(mg/100mL)
Measurement time approx. 10 minutes
Amount of sample 2μL
Measurement wavelength

600nm(Main), 700nm(Sub)

Reference

1) Wu, S. Y. et al.: Acta Pharmacol. Sin., 30, 202 (2009). ※Mouse plasma
2) Uchida, K. et al.: Exp. Anim., 58, 181 (2009). ※Extraction liquid of mouse tissue
3) Ushio, M. et al.: Am. J. Physiol. Endocrinol. Metab., 305, E293 (2013). ※Extraction liquid of mouse tissue
4) Kobayashi, Y. et al.: Biosci. Biotechnol. Biochem., 74, 2385 (2010). ※ Extraction liquid of rat tissue

Product List

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™ Cholesterol for Cellbiology 1,000 tests

294-65801

Keep at 2-10℃

※Not available for sale i n the US.

4. LabAssay™ Creatineline

Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration.

Assay Principle (Jaffé  method)

LabAssay™ Creatinine can be used to measure the creatinin levels in samples by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition.

Kit Contents                     Standard Curve

1 Depoteinizin Reagent 150mL×2vials

2

Picric Acid Reagent for 150mL×2vials
3 0.75mol/L Sodium Hydroxide Solution 10mL×1vial
4 Standard Solution 10mL×1vial

Performance

Standard curve range 2.5~10mg/dL(mg/100mL)
Measurement time approx. 40 minutes
Amount of sample 50μL
Measurement wavelength

520nm

Reference

1) Aranami, F. et al.: J. Med. Invest., 57, 95 (2010). ※ Mouse plasma and urine
2) Toyama, K. et al.: Br. J. Pharmacol., 166, 1183 (2012).  ※Mouse plasma
3) Matsumoto, A. et al.: Environ. Health Prev. Med., 15, 211 (2010). ※Human urine 
4) Nasrin, S. et al.: J. Pharmacol. Sci., 122, 270 (2013). ※Rat urine

Product List

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™ Creatinine for Cellbiology 500 tests

290-65901

Keep at 2-10℃

※Not available for sale in Europe.

5. LabAssay™ Glucose

Sugar is one of the most important sources of energy in biology. It is regulated by various factors within an organism. Glucose converges to a stable ratio of α-form and β-form in solutions.

Assay Principle (Mutarotase・GOD method)

α-D-Glucose is converted to β-D-Glucose by mutarotase. Hydrogen peroxide, which is produced by a reaction between β-D-Glucose and glucose oxidase(GOD), promotes oxidative condensation of phenol with 4-aminoantipyrine quantitatively. LabAssay™ Glucose is a kit used for the quantitative determination of glucose concentrations in samples by measuring absorbance of a red color which is generated by the oxidative condensation reaction.

Kit Contents                     Standard Curve

1 Buffer 150mL×2vials

2

Chromogen Reagent for 150mL×2vials
3 Glucose Standard I 10mL×1vial
4 Glucose Standard II 10mL×1vial

Performance

Standard curve range 2.5~10mg/dL(mg/100mL)
Measurement time approx. 40 minutes
Amount of sample 50μL
Measurement wavelength 505nm(Main), 600(Sub)

Reference

1) Yamashita, Y. et al.: Biosci. Biotechnol. Biochem., 77, 888 (2013). ※Mouse plasma
2) Yamashita, Y. et al.: J. Nutr. Sci., 1, e2 (2012). ※Mouse plasma
3) Wang, W. et al.: J. Renin Angiotensin Aldosterone Syst., 15, 384 (2014). ※Mouse serum
4) Maesako, M. et al.: Neurobiol. Aging, 33, 1011 (2012). ※Mouse serum

Product List

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™ Glucose for Cellbiology 1,000 tests

298-65701

Keep at 2-10℃

6. LabAssay™ NEFA

NEFA (Non‐esterified fatty acid) in the blood is transported complexed with an albumin to peripheral tissues. They are important sources of fuel for the peripheral tissues. The concentration of NEFA in the blood is regulated by a release from the adipose tissues, a consumption in the periphral tissues or a take up from the liver.

Assay Principle (ACS・ACOD method)

NEFA (Non‐esterified fatty acid) forms Acyl-CoA in the presence of Acyl-CoA synthetase(ACS). Hydrogen peroxide, which is produced by a reaction between the Acyl-CoA and Acyl-CoA oxidase(ACOD), promotes oxidative condensation of 3-methyl-N-ethyl-N(-β-hydroxyethyl)-aniline(MEHA) with 4-aminoantipyrine. LabAssay™ NEFA can be used for the quantitative determination of NEFA in samples by measuring absorbance of a blue purple color which is generated by the oxidative condensation reaction.

Kit Contents                     Standard Curve

1 Chromogen Reagent A for 10mL×6vials

2

Solvent A 65mL×1vial
3 Chromogen Reagent A for 20mL×6vials
4 Solvent B 10mL×1vial
5 NEFA Standard Solution
(Oleic acid 1mEq/L)
10mL×1vial

※Oleic acid 1mEq=1mmol

Performance

Standard curve range 0.5~1.97mEq/L
Measurement time approx. 20 minutes
Amount of sample 4μL
Measurement wavelength 550nm

Reference

1) Wang, F. et al.: J. Mol. Endocrinol., 52, 133 (2014). ※Mouse plasma
2) Kobayashi, Y. et al.: Biosci. Biotechnol. Biochem., 74, 2385 (2010). ※Rat plasma
3) Chang, Y. C. et al.: EMBO Mol. Med., 5, 1165 (2013). ※Mouse plasma
4) Uchida, K. et al.: Exp. Anim., 58, 181 (2009). ※Mouse serum

Product List

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™ NEFA for Cellbiology 750 tests

294-63601

Keep at 2-10℃

※Not available for sale in the US and Europe.

7. LabAssay™ Phospholipid

Phospholipids are known as not only as major component of cell membranes but also perform vital functions within the body such as emulsification and absorption of fats or coagulation of blood.

Assay Principle (Choline Oxidase・DAOS method)

Phospholipids are hydrolyzed by Phospholipase D to release hydrogen peroxide. The formed hydrogen peroxide promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay™ Phospholipid is a kit to determine Phospholipid concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Kit Contents                     Standard Curve

1 Buffer 50mL×8vials

2

Chromogen Substrate for 50mL×8vials
3 Standard Solution 10mL×2vials

Performance

Standard curve range 150~596.1mg/dL(mg/100mL)
Measurement time approx. 10 minutes
Amount of sample 2μL
Measurement wavelength 600nm(Main), 700(Sub)

Reference

1) Xu, Q. et al.: Biosci. Biotechnol, Biochem., 77, 1390 (2013). ※Extraction liquid of mouse liver
2) Kuge, H. et al.: J. Biol. Chem., 289, 26783 (2014). ※Liposome
3) Kessler, E. C. et al.: J. Dairy Sci., 97, 5481 (2014). ※Bovine
4) Cheng, L. et al.: Transplantation, 90, 127 (2010). ※Rat

Product List

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™Phospholipid for Cellbiology 1,300 tests

296-63801

Keep at 2-10℃

※Not available for sale in the US.

8. LabAssay™ Tryglyceride

Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides, cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.

Assay Principle

Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase(GPO), promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay ™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Kit Contents                     Standard Curve

1 Buffer 105mL×3vials

2

Chromogen Substrate for 105mL×3vials
3 Standard Solution 10mL×1vial

Performance

Standard curve range 100~888mg/dL(mg/100mL)
Measurement time approx. 10 minutes
Amount of sample 2μL
Measurement wavelength 600nm(Main), 700(Sub)

Reference

1) Saito, M. et al.: Biosci. Biotechnol. Biochem., 76, 2049 (2012). ※Mouse serum
2) Fujimori, K. et al.: Prostaglandins Other lipid Mediat., 94, 96 (2011). ※Extraction liquid of mouse culture cells
3) Zhou, L. et al.: Am. J. Physiol. Renal Physiol., 305, F1491 (2013). ※Rat plasma
4) Wu, S. Y. et al.: Acta Pharmacol. Sin., 30, 202 (2009). ※Rat plasma
5) Wang, F. et al.: J. Mol. Endocrinol., 52, 133 (2014). ※Rat plasma
6) Kato, H. et al.: J. Hepatol., 60, 1032 (2014). ※Extraction liquid of mouse liver

Product List

Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
LabAssay™ Triglyceride for Cellbiology 1,000 tests

290-63701

Keep at 2-10℃

9. Reagents for Adiposity & Diabetes Research

Wako offers a wide variety of products for studying Obesity and Diabetes.

Code No. Product Name Standard curve range Grade Pkg. Size Storage Condition
299-75501GLP-1 ELISA Kit Wako,
High Sensitive
0.94~30pmol/L
(3.1~100pg/mL )
for Diabetes Research96 testsKeep at 2-10℃
293-79301Active GLP-1 ELISA Kit Wako, Chemiluminescent 0.123~30pmol/L for Diabetes Research96 testsKeep at 2-10℃
291-59201Rat GLP-1 ELISA Kit Wako206~50,000pg/mL for Diabetes Research96 testsKeep at 2-10℃
292-60601Rat GLP-2 ELISA Kit Wako0.137~100ng/mL for Diabetes Research96 testsKeep at 2-10℃
299-73801Mouse GIP(Active) ELISA Kit Wako7.8~500pg/mL for Diabetes Research96 testsKeep at 2-10℃
292-80001Glucagon ELISA Kit Wako2.2~143.6pmol/L
(7.8~500pg/mL)
for Diabetes Research96 testsKeep at 2-10℃
291-73501Mouse/Rat PYY ELISA Kit Wako0.15~12.5ng/mLfor Adiposity Research96 testsKeep at 2-10℃
295-57401Rat C-Peptide ELISA Kit Wako1.56~50ng/mL for Diabetes Research96 testsKeep at 2-10℃
297-57601Rat Leptin ELISA Kit Wako78.1~5,000pg/mL
(Non-Plasma、Non-serum)
312.5~20,000pg/mL
(Plasma、Serum)
for Adiposity Research96 testsKeep at 2-10℃

 

 ELISA for Measuring Insulin

Code No. Maker Code Product Name Standard curve range Pkg. Size Storage Condition
637-10629AKRIN-010HLbis Insulin-Rat (H type) 0.5 ~100ng/mL96 testsKeep at 2-10℃
634-10379AKRIN-011HLbis Insulin-Mouse (H type) 0.5 ~100ng/mL96 testsKeep at 2-10℃
631-01479AKRIN-010TLbis Insulin-Rat-T 0.156 ~10ng/mL96 testsKeep at 2-10℃
638-01489AKRIN-011TLbis Insulin-Mouse-T0.156 ~10ng/mL96 testsKeep at 2-10℃
631-07199AKRIN-010SLbis Insulin-Rat (S type)0.1 ~10ng/mL96 testsKeep at 2-10℃
630-07289AKRIN-011SLbis Insulin - Mouse (S type)78 ~5,000pg/mL96 testsKeep at 2-10℃
-AKRIN-130Lbis Insulin-Rat (U-E type) 39 ~2,500pg/mL96 testsKeep at 2-10℃
-AKRIN-031Lbis Insulin-Mouse (U type)39 ~2,500pg/mL96 testsKeep at 2-10℃
630-24149AKRIN-010RULbis Rat Insulin ELISA KIT(RTU) 100 ~12,000pg/mL96 testsKeep at 2-10℃
633-23919AKRIN-011RULbis Mouse Insulin ELISA KIT(RTU)100 ~12,000pg/mL96 testsKeep at 2-10℃
637-01459AKRIN-012TLbis Insulin-Dog-T0.188 ~12ng/mL96 testsKeep at 2-10℃
634-01469AKRIN-013TLbis Insulin-Porcine0.188 ~12ng/mL96 testsKeep at 2-10℃
-AKRIN-014TLbis Insulin-Monkey-T 0.156 ~10ng/mL96 testsKeep at 2-10℃

Other products : Reagent for Adiposity & Diabetes Research

Code No. Maker Code Product Name Standard curve range Pkg. Size Storage Condition
630-23049AKMPI-111Lbis Proinslin Mouse/Rat 1.47~94.3pg/mL
(Standard curve range)
7.35~471.5pg/mL
(Amount of a sample:10μL)
60 testsKeep at 2-10℃
635-07239AKRCP-031Lbis C-Peptide-Mouse
(U type)
46.9~3,000pg/mL
(Standard curve range)
234.5~15,000pg/mL
(Amount of a sample:10μL)
96 testsKeep at 2-10℃
633-07279AKRCP-030Lbis C-Peptide Rat
(U type)
46.9~3,000pg/mL
(Standard curve range)
234.5~15,000pg/mL
(Amount of a sample:10μL)
96 testsKeep at 2-10℃
631-10389AKRLP-011Lbis Leptin-Mouse103~25,000pg/mL
(Standard curve range)
20.6~5,000pg/mL
(Amount of a sample:10μL)
96 testsKeep at 2-10℃
638-04309AKRAL-121Lbis Albumin Mouse
ELISA Kit
50~1,000ng/mL
(Standard curve range)
96 testsKeep at 2-10℃
635-04319AKRAL-120Lbis Albumin Rat
ELISA Kit
50~1,000ng/mL
(Standard curve range)
96 testsKeep at 2-10℃
639-13749AKRGH-010Lbis GH-Rat31.3~2,000pg/mL
(Standard curve range)
313~20,000pg/mL
(Amount of a sample:5μL)
62.6~4,000pg/mL
(Amount of a sample:25μL)
96 testsKeep at 2-10℃
630-23929AKRLH-010SLbis Rat LH
ELISA KIT(S type)
313~10,000pg/mL
(Standard curve range)
1.56~50ng/mL
(Amount of a sample:10μL)
96 testsKeep at 2-10℃
633-31729AKRTS-010S2Lbis TSH-Rat
(SⅡtype)
184~18,000pg/mL
(Standard curve range)
0.92~90ng/mL
(Amount of a sample:20μL)
96 testsKeep at 2-10℃
637-15129AKMGP-011Lbis GLP-1(active)
ELISA KIT
1.56~50pg/mL
(Standard curve range)
7.8~250pg/mL
(Amount of a sample:10μL)
3.9~125pg/mL
(Amount of a sample:20μL)
96 testsKeep at 2-10℃
638-13079AKMAN-011Lbis High Molecular
Adiponectin-Mouse/Rat
3.13~200ng/mL
(Standard curve range)
78.25~5,000ng/mL
(Using sample diluted 25times)
96 testsKeep at 2-10℃
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