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Microglial marker
Labeled Iba1 Antibodies

Iba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 16.7kDa The protein is a commonly known microglial marker in the nervous system. These items are Anti Iba1, Rabbit (for Immunocytochemistry): Wako Cat. No. 019-19741, labeled with Biotin or Red Fluorochrome like Cy5™.

Labeled Iba1 Antibody


1. Link


2. Features

  • The secondary antibody process could be eliminated
  • Reduced background
  • Evaluate by immunohistochemistry test

3. Applications - Immunohistostaining -

Amti Iba1, Rabbit, Biotin-conjugated   Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated
Anti Iba1, Rabbit, Biotin-conjugated   Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated
→ The item shows clearer
    localization signals of
    microglia than using the
    secondary antibody.
■Assay Condition
○Sample
 Cerebral cortex of 7-week Wistar rat
    or 7-week ICR mouse
○Section: 50μm-frozen section
○Staining
 ABC method + DAB staining
○Antibody concentration: 1/200
  → The item shows the
    localization signals of
    microglia as well as using
    the secondary antibody.
■Assay Condition
○Sample
 Cerebral cortex of 7-week Wistar
    rat or 7-week ICR mouse
○Section: 50μm-frozen section

○Antibody concentration: 1/200

All immunohistochemistry data were provided by Sanagi, T., Ichinohe, N. and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

4. Antibody Information

  Anti Iba1, Rabbit, Biotin-conjugated Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated
Conjugate Biotin Red Fluorochrome (Excitation = 634 nm, Emission = 654 nm)
Antigen Synthetic peptide corresponding to C-terminus of Iba1
Buffer PBS with 0.05% Sodium Azide
Antibody class Rabbit IgG
Species cross reactivity Mouse, rat and marmoset Mouse and rat
Application Immunohistochemistry ( 1 : 200 - 2,000 )

5. Product Lists

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Anti Iba1, Rabbit, Biotin-conjugated 100 μL 016-26461 for Immunochemistry Keep at 2 ~ 10°C
Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated 100 μL 013-26471

6. Related Products

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Anti Iba1, Rabbit (for Immunocytochemistry) ⇒ details 50 μg 019-19741 for Immunochemistry Keep at -20°C.
Anti Iba1, Rabbit (for Western Blotting) ⇒ details 50 μg 016-20001
Anti Iba1, Monoclonal Antibody (NCNP24) ⇒ details 10 μg 012-26723
50 μg 016-26721
Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 50 μL 015-25191 for Immunochemistry Keep at -20°C.
Anti Phosphorylated α-Synuclein,
Monoclonal Antibody (pSyn#64), Biotin-conjugated ⇒ details
100 μL 010-26481 Keep at 2 - 10°C.
Anti Phosphorylated α-Synuclein,
Monoclonal Antibody (pSyn#64), FITC-conjugated ⇒ details
100 μL 010-26481
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