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Laboratory Chemicals > Lectins

Next Generation Lectins

Recombinant Lectins

  • Ensure Stable Supplies
  • Ready-to-Use Lectin Solution

Wako has been developing lectins for sugar chain research, aiming to ensure those stable supplies.

The first launch is recombinant BC2LCN (AiLecS1) for research of human induced pluripotent stem (iPS) and embryonic stem (ES) cells. rBC2LCN (AiLecS1) is expected to be applicable to not only stem cell research but also to regenerative medicine since it is reportedly a useful marker for detection of undifferentiated cells.

Application - Detections of an undifferentiation marker of human iPS cells

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rBC2LCN (AiLecS1) from cDNA of Burkholderia cenocepacia

Product Outline

BC2LCN 029-18061 (1 mg), 025-18063 (1 mg x 5)
Glycan specificity Fucα1-2Galβ1-3GalNAc (H type 3)
Fucα1-2Galβ1-3GlcNAc (H type 1)
Formulation PBS (pH 7.4)
cDNA source Synthetic cDNA based on Burkholderia cenocepacia Protein concentration 1.2~1.8mg/ml (BCA Assay)
Source E. coli expressed Burkholderia cenocepacia lectin
(BC2LCN/AiLecS1)
Molecular weight
(theoretical value)
15,807
Purification Affinity purification by fucose agarose. Storage condition Keep at -20°C before use.
  After thawing, keep at 2-10°C.
  Avoid the freeze-thaw cycle.


Detection of undifferentiated iPS cells using rBC2LCN

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Detection of undifferentiated marker in human iPS cell colony naturally differentiated

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<Detection condition>
Fluorescent labeled rBC2LCN
  Final concentration : 0.1-0.5 µg/mL (1 w/v% BSA, 1 x PBS)
  Incubation time : 60 min. (RT, in the dark)
  Cell : Human iPS 201B7 strain
  Fixing condition: 4 w/v% Paraformaldehyde for 10min. at RT or 4°C.

Point in Check
rBC2LCN, a marker of undifferentiated
human ES・iPS cells


rBC2LCN reportedly recognized an intramolecular sugar chain of Podocalyxin, exclusively, which exposes on the cell surface of undifferentiated human iPS cells. Please see the reference 1).
Point in Check
  rBC2LCN (rLectin) Anti TRA-1-81 Mab (Mouse IgM) Anti SSEA-4 Mab (Mouse IgG3)
M. W.(kDa) 16 900 150
Flow Cytometry
Cytological staining
Live cell imaging -
Non-specific binding
(Cytological staining)
Low High Low


rBC2LCN References

1) Tateno, H., Matsushima, A., Hiemori, K., Onuma, Y., Ito, Y., Hasehira, K., Nishimura, K., Ohtaka, M., Takayasu, S., Nakanishi, M., Ikehara, Y., Nakanishi, M., Ohnuma, K., Chan, T., Toyoda, M., Akatsu, H., Umezawa, A., Asashima, M. and Hirabayashi, J. “Podocalyxin is a Glycoprotein ligand of the human pluripotent stem cell-specific probe rBC2LCN.”, Stem Cells Translational Medicine, 2(4), 265-73(2013).

2) Onuma, Y., Tateno, H., Hirabayashi, J., Ito, Y. and Asashima, M. “rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells.”, Biochem. Biophys. Res. Commun., 431(3), 524-9(2013).

3) Tateno, H., Toyota, M., Saito, S., Onuma, Y., Ito, Y., Hiemori, K., Fukumura, M., Matsushima, A., Nakanishi, M., Ohnuma, K., Akutsu, H., Umezawa, A., Horimoto, K., Hirabayashi, J. and Asashima, M. “Glycome diagnosis of human induced pluripotent stem cells using lectin microarray.”, J. Biol. Chem., 286(23), 20345-53(2011).

4) Tateno, H., Onuma, Y., Ito, Y., Hiemori, K., Aiki, Y., Shimizu, M., Higuchi, K., Fukuda, M., Warashina, M., Honda, S., Asashima, M. and Hirabayashi, J. “A medium hyperglycosylated podocalyxin enables noninvasiva and quantitative detection of tumorigenic human pluripotent stem cells.”, Sci. Rep., 4, 4069(2014).



Recombinant Lectin Products

Lectin Groups*1 Name Code No. Package Size cDNA Source
Glycan Specificity *2
Fucose-Complex BC2LCN(AiLecS1) 029-18061 1 mg freezecDNA Source : Burkholderia cenocepacia
Target Glycan : Fucα1-2Galβ1-3GalNAc (H type 3), Fucα1-2Galβ1-3GlcNAc (H type 1)
025-18063 1 mg x 5
AAL 018-25201 1 mg freezecDNA Source : Aleuria aurantia
Target Glycan : Fucose moieties
014-25203 1 mg x 5
PA-IIL 160-26711 1 mg freezecDNA Source : Pseudomonas aeruginosa
Target Glycan : Fucose, Fucose containing oligosaccharides, Mannose
166-26713 1 mg x 5
RS-Fuc 183-03101 1 mg freezecDNA Source : Ralstonia solanacearum
Target Glycan : Fucose
189-03103 1 mg x 5
Glc/GlcNAc-Complex F17AG 062-06281 1 mg freezecDNA Source : E. coli
Target Glycan : GlcNAc
068-06283 1 mg x 5
Malectin 136-18221 1 mg freezecDNA Source : Human
Target Glycan: Glc2-N-biose
132-18223 1 mg x 5
Gal/GalNAc-Complex ABA 015-24851 1 mg freezecDNA Source : Agaricus bisporus
Target Glycan : Galβ1-3GalNAc (T antigen), Galβ1-3GlcNAc (Lacto-N-biose)
011-24853 1 mg x 5
CGL2 033-23771 1 mg freezecDNA Source : Coprinopsis cinerea
Target Glycan : βGal, GalNAcα1-3Gal (Blood Group A), Galα1-3Gal (Blood Group B)
039-23773 1 mg x 5
CNL 039-23631 1 mg freezecDNA Source : Clitocybe nebularis
Target Glycan : α/βGalNAc, GalNAcβ1-4GlcNAc,
 GalNAcα1-3[Fucα1-2]Galβ1-4GlcNAc (Blood Group A)
035-23633 1 mg x 5
Discoidin I 045-33541 0.5mg freezecDNA Source : Dictyostelium discoideum
Target Glycan : αGalNAc (Tn antigen)
041-33543 0.5 mg x 5
Discoidin II 042-33431 0.5 mg freezecDNA Source : Dictyostelium discoideum
Target Glycan : Gal, LacNAc, Asialoglycans, Gal/GalNAcβ1-4GlcNAcβ1-6Gal/GalNAc
Gal1-S 072-06341 1 mg freezecDNA Source : Human
Target Glycan : branched LacNAc
078-06343 1 mg x 5
Gal1, Stable Form 070-06401 50 µg -80cDNA Source : Human
Target Glycan : branched LacNAc, Gal
Gal2 073-06371 1 mg freezecDNA Source : Human
Target Glycan : GalNAcα1-3Gal (Blood Group A), branched LacNAc
Gal3C-S 079-06351 1 mg freezecDNA Source : Human
Target Glycan : poly LacNAc
075-06353 1 mg x 5
Gal3(Full-length) 077-06411 50 µg -80cDNA Source : Human
Target Glycan : poly LacNAc
Gal7-S 076-06361 1 mg freezecDNA Source : Human
Target Glycan : Galβ1-3GlcNAc
072-06363 1 mg x 5
Gal9, Stable Form 074-06421 50 µg -80cDNA Source : Human
Target Glycan : poly LacNAc, GalNAcα1-3Gal (Blood Group A)
LSL-N 127-06361 1 mg freezecDNA Source : Laetiporus sulphureus
Target Glycan : LacNAc, poly LacNAc
123-06363 1 mg x 5
MOA 137-18011 1 mg freezecDNA Source : Marasmius oreades
Target Glycan : Galα1-3[Fucα1-2]Galβ1-4GlcNAc (Blood Group B)
 Galα1-3Galβ1-4GlcNAc, Galα1-3Gal
133-18013 1 mg x 5
PA-IL 167-26721 1 mg freezecDNA Source : Pseudomonas aeruginosa
Target Glycan : Galα1-3(4)Gal
163-26723 1 mg x 5
PPL 168-26751 0.5mg freezecDNA Source : Pleurocybella porrigens
Target Glycan : α/βGalNAc
SRL 199-17271 1 mg freezecDNA Source : Sclerotium rolfsii
Target Glycan : Galβ1-3GalNAc (T antigen), Galβ1-3GlcNAc (Lacto-N-biose)
195-17273 1 mg x 5
Sialic Acid ACG 011-25431 1 mg freezecDNA Source : Agrocybe cylindracea
Target Glycan : α2-3 Sialic Acid
017-25433 1 mg x 5
PSL1a 161-26741 0.5 mg freezecDNA Source : Polyporus squamosus
Target Glycan : α2-6 Sialic Acid
167-26743 0.5 mg x 5
Mannose-Complex BC2L-A 026-18691 1 mg freezecDNA Source : Burkholderia cenocepacia
Target Glycan : High-mannose
Calsepa 031-23831 1 mg freezecDNA Source : Calystegia sepium
Target Glycan : High-mannose
037-23833 1 mg x 5
GRFT 070-06381 1 mg freezecDNA Source : Griffithia sp.
Target Glycan : High-mannose
076-06383 1 mg x 5
PALa 164-26731 1 mg freezecDNA Source : Phlebodium aureum
Target Glycan : High-mannose
160-26733 1 mg x 5
Orysata 159-03281 1 mg freezecDNA Source : Oryza sative
Target Glycan : High-mannose
155-03283 1 mg x 5

*1: Lectin group means one kind of classification of expediency based on the glycan specificity. It doesn’t warrant the recognition ability of all glycans.
*2: The information of the glycan specificity are the common information. These don’t limit the specificity of lectins.



【Product Outline】
  ○Formulation: Liquid
  ○Source:Each lectin cDNA (synthetic) expressed in E. coli.
  ○Storage Condition:-20°C and/or -80°C
 After thawing, keep at 2-10°C. Avoid the freeze-thaw cycle.



Native Lectin Products

Description Code No. Package Size Glycan Specificity
Concanavalin A [Con A] 037-08771 100 mg freezeMan
033-08773 250mg
031-08774 1g
Datura Lectin, Lyophilized [DSA] 047-23231 5 mg freezeGlc, GlcNAc
Dolichos Biflorus Lectin, Peroxidase Labeled [DBA] 047-23731 1 mg freezeGalNAc
Maackia Amurensis Lectin, Lyophilized [MAM] 139-10891 2 mg freezeGal, GalNAc
Peanut Lectin, Lyophilized [PNA] 165-15031 5 mg freezeGal, GalNAc
Phytohemagglutinin-E4, Lyophilized [PHA-E4] 164-15241 5 mg freezeGlc, GlcNAc
Phytohemagglutinin-L4 [PHA-L4] 168-15261 5 mg freezeGlc, GlcNAc
Phytohemagglutinin-P [PHA-P] 161-15251 50 mg freezeGlc, GlcNAc
Sambucus Sieboldiana Lectin, Lyophilized [SSA] 197-10371 5 mg freezeSiaα2-6Gal, Siaα2-6GalNAc
Soybean Lectin, Lyophilized   198-09931 5 mg freezeGal, GalNAc
Wheat Germ Lectin [WGA] 126-02811 10 mg refGlc, GlcNAc


Antibodies to Carbohydrate Antigen

Description Code No. Package Size Clone No. Host Species Isotype Reference
Anti Siaα2-3, Monoclonal Antibody

recognition region: GM3, GM4, α2-3nLc4Cer
011-25171 200 μL HYB4 Mouse IgG3· κ
freeze
1) Hidari, K. et al., BBRC, 436, 394 (2013).
Anti Keratan Sulfate, Monoclonal Antibody

recognition region: Keratan sulfate (lacking oversulfated structure)
011-25811 200 μL R-10G Mouse IgG1
ref
1) Kawabe, K. et al., Glycobiology, 23(3), 322 (2013).
017-25813 1 mL
Anti Sialyl-Tn Antigen, Monoclonal Antibody

recognition region: A cluster composed of Siaα2-6GalNAc-Ser/Thr (Sialyl Tn), Mucin-type glycoprotein
010-25881 100 μL MLS132 Mouse IgG3
ref
1) Fukui, S. et al., Jpn. J. Cancer Res., 79, 1119 (1988).
2) Numata, Y. et al., BBRC, 170(3), 981 (1990).
3) Nakada, H. et al., BBRC, 187(1), 217 (1992).
4) Nakada, H. et al., Proc. Natl. Acad. Sci. USA, 90, 2495 (1993).
5) Tanaka, H. et al., Eur. J. Biochem., 263, 27 (1999).
6) Akita, K. et al., Int. J. Gynecol. Cancer, 22, 531 (2012).
016-25883 500 μL
Anti Tn Antigen, Monoclonal Antibody

recognition region: A cluster composed of GalNAc-Ser/Thr (Tn antigen)
017-25891 100 μL MLS128 Mouse IgG3
ref
013-25893 500 μL
Anti Sialyl-Lea Antigen, Monoclonal Antibody

recognition region: Siaα2-3Galβ1-3[Fucα1-4]GlcNAc (Sialyl Lea), Siaα2-3Galβ1-3GlcNAc
010-25901 100 μL MSW113 Mouse IgG3
ref
1) Kitagawa, H. et al., J. Biochem., 104, 817 (1988).
2) Kitagawa, H. et al., J. Biol. Chem., 268(35), 26541 (1993).
3) Akita, K. et al., Int. J. Gynecol. Cancer, 22, 531 (2012).
016-25903 500 μL

strage




Detection of undifferentiated iPS cells using R-10G antibody

r-10g <Detection condition>
Primary Antibody :
  Upper Section : TRA-1-81 antibody as positive control
  Lower Section : Anti Keratan Sulfate antibody (clone No. R-10G) as test
Secondary Antibody :
  FITC labeled secondary antibodies
Cells: Human iPS cells 201B7 strain.
  Cultured on the feeder cells.

Bulk Supply is available! Please contact WAKO.



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Laboratory Chemicals > Lectins