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Lipase, immobilized, from Pseudomonas sp.

Immobilized lipase is useful for enzymatic ester synthesis, transesterification, acidolysis and alcoholysis in organic solvents or solvent-free synthesis.


Appearance: Lyophilized powder

Activity: min. 0.5 units/mg

Assay:
  1. Principle
       

  2. Procedures
    (1) Pipette 10 mL of dry diisopropyl ether containing 50 mmol/L 2,2,2-Trifluoroethyl Octanoate (TFEO) and 0.2 mol/L ethanol into a test tube with a magnetic stirrer bar and a stopper. Equilibrate at 25.5°C for about 10 minutes with stirring.
    (2) Add about 20 mg of immobilized lipase powder measured accurately and stir constantly.
    (3) Take the 1 μL each of the assay mixture at the 1, 5, 10, 15 minutes after and analyze with capillaly gas chromatograp

  3. Unit Definition
    One unit causes the formation of the 1 μmol of ethyloctanoate (EO) per minute under the conditions described above.

  4. Calculation
    A plot natural logarithm of the reaction conversion (C) vs reaction time gives a straight line [-ln (1 - C) = K0t], and the Initial rate (V0) of the reaction is calculated from the slope of the line.

    Initial rate (V0) = K0 x 50

    K0: Slope of the line [-ln (1 - C) = K0t]
    50: Concentration of TFEO (mM)
    S: Sample weight (mg)
    10: Total volume (mL)
Application - Stereoselective Acylation -
  1. Stereoselective Acylation of Binaphthol
  2. Stereoselective Acylation of Mandelonitrile
  3. Stereoselective Acylation of Tricarbonyl (o-trimethylsilylbenzyl alcohol) chromium
References:
  1. Nakatani, T., Hiratake, J., Yoshikawa, K., Nishioka, T. and Oda, J.: Biosci. Biotech. Biochem., 56, 1118 (1992)
  2. Inagaki, M., Hiratake, J., Nishioka, T. and Oda, J.: Agric. Biol. Chem., 53, 1879 (1989)
  3. Nakamura, K., Ishihara, K., Ohno, A., Uemura, M., Nishimura, H. and Hayashi, Y.: Tetrahedron Letters, 31, 3603 (1990).

Product List
Product name Gade Package Size (Wako Catalgo No.)
Lipase, Immobilized, from Pseudomonas sp. for Biochemistry   1 g    (#124-04171)

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Products listed in this page are for research use only.  Do not administer each to human.



 

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