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for Neural Cell Culturing
N2 Supplement with Transferrin (Holo)
Wako Catalog No. 141-08941 (5 mL)

Leaflet (349 KB/2 p)

Wako's N2 Supplement is used as replacement of general blood serum of neural cell culturing.  Product is suitable for culturing of primary nerve cell and neural stem cell.   Differentiation induction of neural stem cell is triggered by element contained in FBS.
Serum substitutes like N2 Supplement are used for maintaining undifferentiated stage of stem cells during culturing.

INDEX

  1. Components
  2. QC Test
  3. <Data 1> Rat Hippocampus Neural Stem Cell Culture
  4. <Data 2> Rat Hippocampus Neural Stem Cell Sustention
    Neural Differentiation
    Neuroglial Cell Differentiation
  5. Note
  6. LINK ⇒ Information on a brochure "Research for Neurobiohchemistry and Neurological Disease"


1. Components
N2 Supplement with Transferrin (Holo)
Components
CAS No.
Concentration
                   Insulin, recombinant
11061-68-0
500 μg/mL
                   Transferrin (Holo), human
11096-37-0
10,000 μg/mL
                   Progesterone
57-83-0
0.63 μg/mL
                   Putrescine
333-93-7
1,611.00 μg/mL
                   Sodium Selenite
10102-18-8
0.52 μg/mL
                   D-PBS(-)
proper quantity


2. QC Tests:
  1. Sterilization test
  2. Mycoplasma test
  3. Endotoxin test
  4. pH
  5. Osmic
  6. Cell Culture test


3. <Data 1> Rat Hippocampus Neural Stem Cell Culture
Fig/Comparison: Rat Hippocampus Neural Stem Cell Culture



<Medium Composition>

   D-MEM/Ham's F-12 + 2 mmol/L L-Glutamine
   + 1 x N2 Supplement + 1 x Penicillin-Streptomycin
   + 25 ng/mL bFGF

<Seeding Cell Number>

   1,600 cells/cm2 (6,000 cells/well, 12-well plate)

<Culture Condition>

   37°C , 5% CO2
4. <Data 2> Rat Hippocampus Neural Stem Cell Sustention-Neural Differentiation- Neuroglial Cell Differentiation
containing N2 Supplement with Transferrin (Holo) x 100 (Wako Cat. #141-08941)
Neural Stem Cell
(25 ng/mL bFGF)
Fig/Neutral Stem Cells
Neuron Differentiation
(1 μM RA + 5 μM Forskolin)
Fig/Neuron Differentiation
Astrocyte Differentiation
(50 ng/mL BMP2 + 5000 U LIF)
Fig/Astrocyte Differentiation
Fig/Neutral Stem Cells: Sox2/DAPI Fig/Neuron Differentiation: Tuj1/DAP1 Fig/Astrocyte Differentiation: GFAP/DAPI
Sox2: a marker of undifferentiated embryonic stem cells TuJ1: a neuronal differentiation marker GFAP: an strocyte marker
DAPI: a DNA marker
Expression of each marker was confirmed by immunostaining.

Product List
Product Name Grade Package Size Wako Catalog No.
N2-Supplement with Transferrin (Holo) (x 100) for Cell Culture 5 mL 141-08941
N2-Supplement with Transferrin (Apo) (x 100) for Cell Culture 5 mL 141-09041
(Note)
Precipitation may form during storage at -20°C.  Even so, please use it without any pretreatment since there is no problem in use.




 

 
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