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Laboratory Chemicals > Product Information > N > N2 Supplement

for Culture of Neural Stem Cells
N2 Supplement with Transferrin (Apo)


This product is an alternative for serum used in the culture of nerve cells. It is suited to primary culture of nerve cells and neural stem cells. Differentiation of neural stem cells is induced by the ingredients in FBS. Serum alternative such as this product is used to culture stem cells while keeping the cells undifferentiated.
This product is prepared using transferrin (apo). Transferrin (holo) is used generally but addition of N2 supplement containing transferrin (apo) may improve proliferation of neural stem cells depending on cell species.



 INDEX
  1. Quality test
  2. Composition
  3. Practical data (1): Culture of rat hippocampus-derived neural stem cells
  4. Practical data (2): Maintenance/differentiation of rat hippocampus-derived neural stem cells
  5. Related products
    · Products for Cell Culture
    · Reagents for ES / iPS Cell Research
  6. LINK ⇒ Information on a brochure "Research for Neurobiohchemistry and Neurological Disease"



1. Quality test

- Sterility test
- pH
- Osmotic pressure
- Bacterial endotoxin test
- Mycoplasma test
- Cell culture test



2. Composition
Ingredient CAS No. Concentration (mg/L)
Insulin, human, recombination product 11061-68-0 500.00
Transferrin, human origin 11096-37-0 10,000.00
Progesterone 57-83-0 0.63
Putrescine dihydrochloride 33-93-7 1,611.00
Sodium selenite 10102-18-8 0.52
* Code No. 141-08941 N2 supplement [containing transferrin (holo)] is prepared without D-PBS.
Precipitation may occur during storage but has no problem in the use.




3. Practical data (1): Culture of rat hippocampus-derived neural stem cells

● N2 supplement with Transferrin (Apo) [Wako Catalog No.141-09041]
Addition of N2 supplement with Transferrin (Apo) may improve proliferation of neural stem cells depending on cell species.
● N2 supplement with Transferrin (Holo)] [Wako Catalog No.141-08941]
Composition used conventionally.
It shows cell proliferation comparable to that of an equivalent product of Company A when added to a medium.



4. Practical data (2): Maintenance/differentiation of rat hippocampus-derived neural stem cells


● Maintenance of neural stem cells
Maintenance of stem cells was confirmed by the expression of Sox2 after addition of 25 ng/mL bFGF.

● Differentiation to nerve cells and glia cells
[Nerve cells]: Differentiation to nerve cells was confirmed by the expression of TuJ1
after addition of 1 μM retinoic acid and 5 μM forskolin.
[Glia cells]: Differentiation to glia cells was confirmed by the expression of GFAP after addition of 10% FBS and 50 ng/mL LIF.

Product Name Grade Package Size Wako Catalog No.
N2 Supplement with Transferrin (Apo) (x 100) for Cell Culture 5 mL 141-09041
N2 Supplement with Transferrin (Holo) (x 100) for Cell Culture 5 mL 141-08941



5. Related products
· Products for cell culture
Full line-up of products such as balanced salt solutions, trypsin-EDTA solutions, antibiotic solutions, extracellular matrices in addition to liquid media are available. ⇒ Link

· Reagents for ES / iPS Cell Research
Many literatures related iPS cells have been published since establishment of iPS cells in 2007. We offer low-molecular compounds that have been reported to associate with maintenance of the state of undifferentiation and induction of differentiation of ES cells and iPS cells in various literatures. ⇒ Link

Note

  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
  • Please visit Wako's online catalog to search for other products available through Wako: www.e-reagent.com
  • This information may contain products that cannot be exported to your country due to regulations.
  • Bulk quote requests for some products are welcomed. Please contact us.
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