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for the culture of nerve cells and neural stem cells
NS Basal Medium / NS supplement

NS basal medium is the optimized basal medium for nerve cell culture.
NS supplement is the serum-free supplement (containing retinyl acetate) for nerve cell culture.
They can be used for the culture of the nerve cells isolated from the rat hippocampus and neural stem cells. Please use a mixture of NS basal medium and NS supplement.


1. Link


2. The culture of primary nerve cells derived from rat hippocampus.

The nerve cells isolated from the hippocampus of the rat fetal (E19) were cultured on the poly -L- lysine coated plate.
We confirmed the cell morphology on culture day 6, the expressions of neuronal markers (Map2 (a + b)) and glial cell marker (GFAP) on culture day 21.

Cultured day 6

Culture Day 6 <Culture medium composition>
NS basal medium + 2% NS supplement
+ 0.5 mmoL/L L-Glutamine

<The number of cell seeding>
13,000 cells/well (96 well plate)

Cultured day 21

Culture Day 21


Product Name Package Size Wako Cat. No. Storage Condition Grade
NS Basal Medium 500 mL 148-09615 Keep at 2-10°C. for Cell Culture
NS Supplement (x 50) 10 mL 146-09351 Keep at -20°C.

3. The culture of primary nerve cells derived from rat cerebral cortex

The primary nerve cells isolated from cerebral cortex of the rat fetal (E17) were cultured on the poly -L- lysine coated plate.
We confirmed the cell morphology on culture day 8, the expressions of neuronal markers (Map2 (a + b)) and glial cell marker (GFAP) on culture day 14.

Cultured day 8

Culture Day 8 <Culture medium composition>
NS basal medium + 2% NS supplement
+ 0.5mmoL / l L- Glutamine

<The number of cell seeding>
60,000cells / well (96 well plate)

Cultured day 14

Culture Day 14

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
200 mmol/L L-Glutamin Solution (x 100) 100 mL 073-05391 for Cell Culture Keep at -20°C.
N2 Supplement with Transferrin (Holo) 5 mL 141-08941
N2 Supplement with Transferrin (Apo) 5 mL 141-09041

*Since the additive amount of Fe ions in the culture medium is suppressed by N2 Suppelment with Transferrin (Apo), it might be suitable for the cells weak to oxidative stress.

4. Related Products

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
N2 Supplement without Insuline (x 50) 10 mL 149-09721 for Cell Culture Keep at -20°C,
50 mL 145-09723
N2 Supplement with Vitamin A (x 50) 10 mL 142-09691
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