Novel Affinity Tag System for Animal Host cells! - From Japan Next Generation Affinity Tag System version 2
|PA tag is a versatile protein tagging system using a super high affinity monoclonal antibody (NZ-1) against a peptide tag (12 amino acids).
Monoclonal antibody (NZ-1)has high affinity and high specificity against PA tag and is applicable to Immunocytochemistry (ICC), Western blotting and flow cytometry.
In addition, purification of PA tag fusion protein by IP and regeneration of beads are available.
PA tag system will be a unique and powerful tool for production in animal host cells.
We also offer a lineup of products of other vectors with antibiotic resistance genes different from the above (Please see the p.4 of this leaflet.). Detailed information on the sequencing primers and other vector sequences are included in the instruction manual supplied with the product. The sequence information (compressed file) and Multi Cloning Site (MCS) can be downloaded from our website.
|NZ-1 is a monoclonal antibody against PA tag (GVAMPGAEDDVV). NZ-1 can be used to detect recombinant PA tag fusion proteins expressed in animal cells and Escherichia coli. The antibody may provide some background signals in application to certain cell lines expressing human podoplanin at a high level, such as the human glioblastoma cell line LN319. The absence of cross reactivity has been confirmed in mouse-, rat-, hamster-, and dog-derived cell lines.|
Indicated on the label.
(Approximately 1.0 mg/mL)
1× PBS (pH 7.5) aqueous solution
Western Blot 0.01-1 μg/mL
Immunoprecipitation 10-50 μg/assay
Flow Cytometory 0.1-10 μg/mL
Immunocytochemistry 0.1-10 μg/mL
Keep at 2-10°C.
Avoid repeated freeze and thaw.
Data was provided by Junichi Takagi and Yuki Fujii, Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University
|Recombinant isocitrate dehydrogenase (IDH1), tandemly fused with DYKDDDDK tag and PA tag at the C terminus, was expressed in osteosarcoma cells and detected with antibodies specified below, following SDS-PAGE of the cell lysate.|
Data were provided by Drs. Yukinari Kato and Mika Kaneko, Regional Innovation Center, Tohoku University Graduate School of Medicine.
Lane 1 Primary Ab: Anti IDH1 Ab (RcMab-1), 1 µg/mL
|NZ-1 showed a high detection sensitivity
comparable with that of native antibodies.
These products are gene expression vectors for mammalian cells. They enable expression of target proteins fused PA tag.
Notice to purchaser: These products are for research use only. If you would like to resell them and/or develop new vectors constructed by DNA fragments and/or sequence information of the products, please contact us (firstname.lastname@example.org).