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for Insect Cell Culture for Sf9, Sf21 and H5 cells
PSFM-J1 Medium Wako, Liquid

Here we provide versatile serum-free medium for insect cells.  No need to worry about change in biological activity for each serum lot and virus contamination because of serum-free.  Also no need to add L-glutamine in High Five (H5) cell culture.

  • Optimized for protein expression by Sf9 and High Five (H5) cells, respectively
  • Small lot-to-lot variation
  • No need of additives such as L-glutamine

INDEX

  1. Sf21 cell culture Data
  2. Sf9 cell culture Data
  3. High Five(H5) cell culture Data
  4. Product List
  5. Related Products
  6. LINK
    Leaflet (395 KB)
    ES·iPS Cell Culture Catalog

1. Sf21 cell culture Data








2. Sf9 cell culture Data








3. High Five(H5) cells


Cautions associated with switch over to the medium

  1. Prepare conditioned medium when you switch from the present medium.
    e.g.) Use the product by gradually increasing the percentage of this product to the present medium such as 10%→30%→50%→70%→90%→100%.
  2. No need to add L-glutamine in High Five (H5) cell culture.



4. Product List

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
PSFM-J1 Medium Wako, Liquid 500 mL 162-25855 for Cell Culture Keep at 2-10°C.
1 L 160-25851



5. Related Products

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Cholesterol, Semi-synthetic 5 g 034-22961 for Cell Culture Keep at 2-10°C.
25 g 032-22962
Polyoxyethylene Polyoxypropylene Glycol (160E.O.) (30P.O.) 100 g 160-25231 for Biochemistry Keep at RT.
500 g 162-25235

      (polyacrylamide gel SuperSep Ace)

Product Name Well Molecular weight cutoff ranges Pkg. Size Wako Cat. No. Storage Condition
SuperSep Ace, 6% 13 70,000 - 240,000 10 gels 195-15171 Keep at 2-10°C.
SuperSep Ace, 7.5% 13 40,000 - 200,000 10 gels 198-14941
17 10 gels 191-14931
SuperSep Ace, 10% 13 20,000 - 130,000 10 gels 195-14951
17 10 gels 192-14961
SuperSep Ace, 12.5% 13 14,000 - 80,000 10 gels 199-14971
17 10 gels 196-14981
SuperSep Ace, 15% 13 6,000 - 60,000 10 gels 193-14991
17 10 gels 190-15001
SuperSep Ace, 5-12% 13 25,000 - 240,000 10 gels 199-15191
17 10 gels 192-15201
SuperSep Ace, 5-20% 13 10,000 - 200,000 10 gels 197-15011
17 10 gels 194-15021
SuperSep Ace, 10-20% 13 10,000 - 130,000 10 gels 191-15031
17 10 gels 198-15041
SuperSep Ace, 15-20%
(Tricine Gel)
13 3,500 - 50,000 10 gels 191-15301
17 10 gels 198-17361



Each electrophoresis pattern of the above mentioned SuperSep Ace using a Wako's protein marker "WIDE-VIEW™ Prestained Protein Size Marker III (Wako Catalog No. 230-02461 (500μL))".

Electrophoresis pattern of SuperSep Ace
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