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Laboratory Chemicals Division > Product Information > Phos-tag™ series > Phos-tag™ Acrylamide AAL-107
Phos-tag™ - based Mobility Shift Detection of Phosphorylated Proteins
- Phosphate Affinity SDS-PAGE using Acrylamide-pendant Phos-tag™ -
Phos-tag™ Acrylamide AAL-107

Phos-tag™ Acrylamide

Phos-tag™ is a novel phosphate-binding tag at neutral pH* (physiological pH) and was developed by Department of Functional Molecular Science at Hiroshima University. Wako Distributes specific electrophoretic procedure for the simultaneous analysis of a phosphoprotein isoform and its non-phosphorylated counterpart Manganese (II)-Phos-tag™ SDS-PAGE using thePhos-tag™ technologies are innovative methods using Pho-tag™ Agarose for separation, purification and enrichment of phosphorylated substances.

   *: In case of Zn2+ is used as M2+



<Basic structure of Phos-tag™>


M2+: Zn2+ ion or Mn2+ ion

  • <Zn2+-Phos-tag™>:
    a stable complex is formed with a phosphate group at the ratio of 1 to 1 at pH 5 ~ 8.

  • <Mn2+-Phos-tag™>:
    a stable complex is formed with a phosphate group at the ratio of 1 to 1 at around the pH 9.
Phos-tag™ Acrylamide is manufactured by NARD Institute.

LINK to Phos-tag™ PAGE applications
Phos-tag was advertised in nature
on Sep. 25, 2104 and Jan. 29, 2015.
⇒ zoom
 INDEX
  1. Features
  2. Principle of phosphorylation of Affinity Electrophoresis
  3. Application: Time course of phosphorylation by using the Tyrosine Kinase Abl

  4. Product List
    • Phos-tag™ Acrylamide
    • Phos-tag™ Acrylamide 5 mM Aqueous Solution
    • Precast Gel: SuperSep™ Phos-tag™ (50 μmol/L)

  5. Related Products - Ready-to-Use Precast Gel
  6. References
  7. LINK        ⇒ GUIDEBOOK issued in Sep. 2016  
                     ⇒ Manual (Ver. 12) issued in Aug. 2014 PDF (1,085 KB/13 pages)
                     ⇒ Leaflet updated in Sep. 2016 PDF (1.113 KBB/4 pages)

Phos-tag™ Acrylamide AAL-107
1. Features
(1) Staining
  • Suitable for kinase assay
  • Almost the same basic mechanism as that of SDS-PAGE
(2) Western Blotting
  • No need to prepare an antibody against the phosphorylated protein
  • Applicable for phosphorylation analysis of endogenous proteins
  • Radioisotope labeling is not required



GUIDEBOOK for the First Phos-tag™ Acrylamide Trial Please visit the guidebook page for the First Phos-tag™ 
    Acrylamide Trial



Product Information shown in a journal "Proteomics Research 2012" Please see a journal-Proteomics Research 2012, in which you can 
    see the Phos-tag™ pages
  Phos-tag™ Acrylamide Aqueous Solution is now available! Recommendable for the first 
    Phos-tag™ Acrylamide Trial



2. Principle of Phosphate Affinity Electrophoresis

Principle of phosphate affinity electrophoresis



3. Application: Time course of phosphorylation by using the Tyrosine Kinase Abl
        
Conventional SDS-PAGE (CBB staining)


Phos-tag™ SDS-PAGE (CBB saining)

Phosphorylated tyrosine was prepared by GST binding protein of tyrosine kinase Abl and the substrate peptide (Abltide) and separated with conventional SDS-PAGE and Phos-tag. SDS-PAGE, respectively.


Phosphorylated proteins and non-phosphorylated proteins were inseparable with the conventional SDS-PAGE (above figure: in the absence of Phos-tag™).

On the other hand, Phos-tag™ SDS-PAGE (below figure: 100 μM Phos-tag™ Acrylamide) can identify the time-course ratio of phosphorylated and corresponding dephosphorylated proteins in a polyacrylamide gel.



4. Product List
Description Package Size Wako Catalog No.     (Manufacturer's Code)
Phos-tag™ Acrylamide 10 mg 304-93521            (AAL-107)
2 mg 300-93523         (AAL-107M)


Phos-tag™ Acrylamide Aqueous Solution
Description Package Size Wako Catalog No.     (Manufacturer's Code)
Phos-tag™ Acrylamide 5 mM Aqueous Solution 0.3 mL (0.9 mg) 304-93526       (AAL-107S1)


5. Related Products

            Ready-to-Use Precast Gel
            SuperSep Phos-tag™ ⇒ Link

6. References


7. LINK
Note
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Laboratory Chemicals Division > Product Information > Phos-tag™ series > Phos-tag™ Acrylamide AAL-107

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