for stimulation of cell proliferation, suppression of cell death, and cryopreservation of cells
Pure Sericin
TM
A protein derived from silkworms
Wako Catalog No. 167-22681 (1 g)
163-22683 (5 g)
Pure Sericin
is a protein derived from the silkworm cocoon, which is produced from Seiren Co., Ltd.. It has been
reported that an addition of 0.1 % (w/v) sericin to the media stimulates cell proliferation and suppresses cell-death.1), 2)
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Furthermore, when 1 % (w/v) sericin and DMSO are added to the media, it can be used as a serum free freezing
medium.
3)
It is applicable as a useful component of serum free media.
Application for monoclonal antibody production system of hybridoma cell
The following three conditions for media were compared: Proliferation of hybridomas was examined in Daigo's T Medium (Nihon Pharmaceutical
Co., Ltd.; Wako Cat. #395-00581, 10x for 1 L) as a basal medium. 1) Daigo's T Medium only, 2) Daigo's T Medium with 0.1 % sericin, 3) 10 % FBS-
added Daigo's T Medium. As a result, Daigo's T Medium with 0.1 % sericin improved viable cell density and viability more efficiently compared to
Daigo's T Medium only (Figure 1, Figure 2). In addition, antibody production competence in Daigo's T Medium with 0.1 % sericin and with 10 %
FBS are equivalent (Figure 3).
(Data: Provided by Satoshi Terada, Department of Applied Chemistry and Biotechnology, University of Fukui, Japan)
Examination of freezing at -80 ºC and thawing, a survey on human hepatoblastoma HepG2 cell line
HepG2 cells were suspended in the following freezing solution to make each vial
to contain approximately 20 X 10
4
cells. The vials were kept frozen for 3 days
at -80 ºC. After thawing, the cells were incubated for three days and the number
of viable cells was counted.
1. General freezing solution (90 % FBS + 10 % DMSO)
2. Sericin solution (PBS solution containing amino acids, 1 % sericin,
and 10% DMSO)
(Provided by Satoshi Terada, Department of Applied Chemistry and Biotechnology,
University of Fukui, Japan)
References
1) Terada, S., Nishimura, T., Sasaki, M., Yamada, H. and Miki, M.: Cytotechnology, 40, 3 (2002).
2) Terada, S., Sasaki, M., Yanagihara, K. and Yamada. H.: J. Bioscience and Bioengineering, 100, 667 (2005).
3) Sasaki, M., Kato, S., Yamada, H. and Terada, S.: Biotechnol. Appl. Biochem., 42, 183 (2005).
Stimulation of proliferation for various cells
Wako Cat. No.
Description
Grade
Pkg. Size
167-22681
163-22683
1 g
5 g
Pure SericinTM
for Cell Culture
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