Keep your valuable RNA samples active long term!
Irreversible RNase Inactivation Reagent
RNAstabilizer
RNAstabilizer
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Wako Catalog No. 180-01891
RNAstabilizer is used when RNA is purified from various organs and for improving the
stability of obtained RNA. RNAstabilizer is particularly useful for the purifcation of high
quality RNA from the pancreas or liver. When RNAstabilizer is applied to an existing RNA
purification kit, which utilizes a carrier such as silica for adsorption and filtration of nucleic
acids, it irreversibly inactivates the RNase derived from smples. High quality RNA with
excellent stability can be obtained.
Since RNase is inactivated irreversibly, valuable samples
can be kept long term.
Since reactivation of inactivated RNase is inhibited by
reducing agents such as mercaptoethanol, the RNase
inactivation is irreversible.
RNA with high stability can be purified from the RNase
rich organs such as liver, pancreas and kidney.
High quality RNA can be obtained with no effect on
recovery by application to an existing RNA purification Kit,
which utilizes a carrier such as silica for adsorption and
filtration of nucleic acids.
Total RNA was extracted from mouse liver and pancreas using InvisorbTMSpin Tissue RNA Mini Kit (Invitek).
Extracted RNA was incubated at 37 ºC overnight. Electrophoretograms are given below. Washing buffer: 50 µL of
RNAstabilizer was added to 500 µ L of Wash Buffer R1 (first washing buffer) and extraction was carried out.
Directions for use
: RNAstabilizer is applied to the washing step of a RNA purification kit, which
utilizes a carrier such as silica for adsorption and filtration of nucleic acids.
As for RNA adsorption to the carrier, please consult the manufacture's
instructions of each purification kit.
Add stabilizer solution to
washing buffer(x1/10 volume)
Wash with washing buffer
containing stabilizer solution
Allow to stand at room
temperature for 20 min
Wash with washing buffer
alone
Elute
1/10 volume of RNAstabilizer is added to the first washing buffer. Washing
steps usually consist of 2 steps.
In the first washing step after adsorption, washing buffer with RNAstabilizer is
applied to a spin column.
After 20 min, the column is centrifuged according to the manufacture's
instructions.
In the second washing step, the column is washed without RNAstabilizer
according to the manufacture's instructions.
Subsequent steps are carried out according to the manufacture's instructions.
Total RNA was extracted from mouse liver using RNA extraction kit (Company Q). Extracted RNA was incubated at
37ºC overnight. An Electrophoretogram is given below.
Washing buffer: 70 µL of RNAstabilizer was added to 700 µL of Buffer RW1 (first washing buffer) and extraction was
carried out.
Description
Wako Catalog No.
Package size
RNAstabilizer, Irreversible RNase Inactivation Reagent
1 bottle x 3.5 mL (methanol solution)
180-01891
50 reactions
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