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FUJIFILMFUJIFILM Wako Pure Chemical Corporation Laboratory Chemicals
Home > Products > Life Science >  Culture  >  PrimeSurface®

Bringing 3D cells culture for reliable anticancer drug screenings and regenerative medicine
PrimeSurface®

1. Application

For Medical Research Institution/Laboratory

Cell Culture:

For Stem Cell Research:
Induction of ES, iPS and mesenchymal stem cells differentiation by embryoid body formation.

For Drug Screening Research & Development:
An ideal product for drug response studies. Three dimension spheroid models are more physiologically relevant than two dimension monolayer model.

2. Features

·Sterile, Ready-to-use, Individually packed
·Promotes uniform size, Single spheroid in one well
·Luminescent detection on same plate
·High volume well capacity
·Compatible with liquid handling systems

3. Biocompatible hydrophilic polymer on the surface promotes cell aggregation

Single spheroid can be formed in each well Spheroid formation can be observed easily

4. Three well bottom shapes of 96 well microplate

Seeding Density: 2x103 cells/well,
Culture Medium: RPMI + 10%FBS,
Incubation: 37℃, 5%CO2,Culture
Period: 7 Days
MDA-MB-453,MDA-MB-468:human breast cancer

Data are provided by Nishio Lab., Dept. of Genome Bio. Kinki Univ. Faculty of Medicine

5. Application example : The difference of drug efficacy mode between 5-FU and TPZ

Figure 1. Comparison of mode of action between 5-FU and TPZ.
Figure 2. Comparison of Cell viability curve between 5-FU and TPZ
TPZ showed stronger effect in “Spheroid” than “Monolayer”. This experiment suggest 3D drug efficacy test environment could be reproduced with “PrimeSurface”.

6. Supplemental data - Video

 

Time-lapse imaging of human iPS cells spheroid (embryoid body) formation

 

Click
Culture conditions
Culture plate PrimeSurface MS-9096V
Cells hiPS cell (201B7: Takahashi K et al. Cell, 2007 Nov 30;131(5):861-72, iPS Academia Japan, Inc.)
Seeding density 9,000 cells/well
Culture medium DMEM/F12 + 20% (v/v) KSR + 1% (v/v) NEAA + L-Glutamine (2mM) + Β-Mercaptethanol (80μM) + Y-27632(30μM)
Culture environment 5%CO2, 37°C

 

Time-lapse imaging of HeLa cells spheroid formation in 96 well plate

 

Click
Culture conditions
eding density 1,000 cells/well
Culture medium MEM+10FBS 100μL/well
Culture period 48hours

 

Time-lapse imaging of HepG2 cells spheroid formation in 384 well plate

 

Click
Culture conditions
eding density 1,000 cells/well
Culture medium DMEM (Low Glucose) + 10%FBS 50μL/wel
Culture period 48hours

7. Site lists

8. Product lists

Microplate
Wako Cat No.
S-Bio Cat No.
Product Name Well
type
color Well
bottom
Maximum
well
Package size
627-01419
MS-9024XZ
PrimeSurface® 24 well24ClearFlat
(1.8cm2)
3.4 mLIndividual package
10 plates/case
635-21039
MS-9096UZ
PrimeSurface® 96U96ClearRound300 μLIndividual package
20 plates/case
621-01439
MS-9096WZ
PrimeSurface® 96W96WhiteRound300 μLIndividual package
20 plates/case
622-01109
MS-9096MZ
PrimeSurface® 96M 96ClearSpindle200 μLIndividual package
20 plates/case
629-01099
MS-9096VZ
PrimeSurface® 96V96ClearV300 μLIndividual package
20 plates/case
628-01449
MS-9384UZ
PrimeSurface® 384U384ClearRound106 μLIndividual package
20 plates/case
625-01459
MS-9384WZ
PrimeSurface® 384W384WhiteRound106 μLIndividual package
20 plates/case

 

Dish
Wako Cat No.
S-Bio Cat No.
Product Name color Well
bottom
Package size
634-28709
MS-9035XZ
PrimeSurface® dish 35mm ClearFlat
(9cm2)
5/package
50/ case
631-28719
MS-9060XZ
PrimeSurface® dish 60mm ClearFlat
(21cm2)
10/package
100/ case
638-28729
MS-9090XZ
PrimeSurface® dish 90mm ClearFlat
(57cm2)
10/package
50/ case

[Remark]
Radiation sterilized
Preserved at room temperature
Product life: 2 years after production

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