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FUJIFILMFUJIFILM Wako Pure Chemical Corporation Laboratory Chemicals
Home > Products > Life Science >  Culture  >  ProteoSave™

For your precious sample preservation
ProteoSave™

1. Features

✔ Coated with an unique ultra-hydrophilic polymer which offers minimized protein denaturation and non-specific protein adsorption

✔ Provides no eluted materials, heat- and cold-resistance, and chemical-resistance due to covalently bonded coating

2. Application example in analysis of small molecules

Comparison of Non-ionic small compounds with non-coated tube

Experimental conditions
Vessel ProteoSave™ 1.5 mL Microtube
Non-coated 1.5 mL Microtube
Concentration 0.1 mg/mL
Sample Non-ionic small molecule
1.Digoxin(Anti-anginal drug)
2.Paclitaxel(Anti-cancer drug)
Measurement After 1 h incubation with non-ionic small molecule, determined the concentration with LC-MS/MS

※Provided by SEKISUI MEDICAL CO., LTD., JAPAN

3. Application example in Proteomics Research

Comparison of the number of identified peptides with competitive products

Experimental conditions
Vessel ProteoSave™ 1.5 mL Microtube 5 competitive tubes
Sample Peptides derived from Hep3B
Quantity 300 μg
Method nanoLC-Ultra 2D with TripleTOF®5600, AB SCIEX
Column 75 mm×150 mm,ChromXP C18-CL
3 μm 120 Å Ekisigent
Eluent A: 0.1% FA and 1% ACN
B: 0.1% FA and 99% ACN
Software Mascot Server

※ Provided by Dr. Masahiro Kamita, Ph.D, National Cancer Center Research Institute, JAPAN.

4. Protein Adsorption to vessel

Diluted enzyme-labeled antibody solutions from 0.1-0.8 mg/mL with phosphate buffer, and dispensed diluted the solutions into non-coated tube, competitive tube and ProteoSave™. After 1 h incubation at 37°C, washed by phosphate buffer, then the amount of adsorbed enzyme-labeled antibodies was measured by microplate reader.

5. Chemical, Heat and Cold Resistant

Organic solvent 10%, 5hr
Methanol
Ethanol
2-propanol
Glycerol
Acetonitrile
Acetone
DMSO
2-mercaptoethanol
Surfactant 0.1%
CHAPS
Triton X-100
Tween 20
SDS
Heat resistant Cold resistant
100 °C (10 min) -80°C (3 months)

 

6. Site lists

7. Product lists

Centrifuge tube
15 mL & 50 mL
Micro tube
0.5 mL & 1.5 mL
Slim tube
0.5 mL & 1.5 mL
96-deep wells plate 96-wells flat & U bottom plate

 

Wako Cat No.
S-Bio Cat No.
Product Name Material Note Qty/
Pk
Qty/
Cs
638-27509
MS-4205MZ
ProteoSave™ 0.5 mL
Microtube
PolypropyleneNon-sterilized100500
632-27529
MS-4255MZ
ProteoSave™ 0.5 mL
Microtube (Sterilized)
PolypropyleneRadiation sterilized100500
635-27519
MS-4215MZ
ProteoSave™ 1.5 mL
Microtube
PolypropyleneNon-sterilized100500
639-27539
MS-4265MZ
ProteoSave™ 1.5 mL
Microtube (Sterilized)
PolypropyleneRadiation sterilized100500
MS-4220MZProteoSave™ 2 mL
Microtube
PolypropyleneNon-sterilized100500
MS-4270MZProteoSave™ 2 mL
Microtube (Sterilized)
PolypropyleneRadiation sterilized100500
638-27489
MS-4201XZ
ProteoSave™ 0.5 mL
Slimtube
PolypropyleneNon-sterilized50500
635-27499
MS-4202XZ
ProteoSave™ 2 mL
Slimtube
PolypropyleneNon-sterilized50500
630-28549
MS-8296FZ
ProteoSave™ 96-wells
Flat Plate
PolystyreneNon-sterilized550
MS-8296KZProteoSave™ 96-wells
Black, Flat Plate (Made to order)
PolystyreneNon-sterilized550
639-29599
MS-8296VZ
ProteoSave™ 96-wells
V Plate
PolypropyleneNon-sterilized520
MS-82962ZProteoSave™ 96-deep wells
V Plate, 2mL
PolypropyleneNon-sterilized345
639-27419
MS-3296UZ
ProteoSave™ 96-wells
U Plate
PolystyreneNon-sterilized550
638-28109
MS-52150Z
ProteoSave™ 15 mL
Conical Tube
PETNon-sterilized5100
635-28119
MS-52550Z
ProteoSave™ 50 mL
Conical Tube (Sterilized)
PolypropyleneRadiation sterilized5100
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