For your precious sample preservation
ProteoSave™

• 1. Features
• 2. Application example in analysis of small molecules
• 3. Application example in Proteomics Research
• 4. Protein Adsorption to vessel
• 5. Chemical, Heat and Cold Resistant
• 6. Site lists
• 7. Product lists

1. Features

✔ Coated with an unique ultra-hydrophilic polymer which offers minimized protein denaturation and non-specific protein adsorption

✔ Provides no eluted materials, heat- and cold-resistance, and chemical-resistance due to covalently bonded coating

2. Application example in analysis of small molecules

Comparison of Non-ionic small compounds with non-coated tube

	Experimental conditions Vessel ProteoSave™ 1.5 mL Microtube Non-coated 1.5 mL Microtube Concentration 0.1 mg/mL Sample Non-ionic small molecule 1.Digoxin（Anti-anginal drug） 2.Paclitaxel(Anti-cancer drug) Measurement After 1 h incubation with non-ionic small molecule, determined the concentration with LC-MS/MS

※Provided by SEKISUI MEDICAL CO., LTD., JAPAN

3. Application example in Proteomics Research

Comparison of the number of identified peptides with competitive products

Experimental conditions Vessel ProteoSave™ 1.5 mL Microtube 5 competitive tubes Sample Peptides derived from Hep3B Quantity 300 μg Method nanoLC-Ultra 2D with TripleTOF®5600, AB SCIEX Column 75 mm×150 mm,ChromXP C18-CL 3 μm 120 Å Ekisigent Eluent A: 0.1% FA and 1% ACN B: 0.1% FA and 99% ACN Software Mascot Server

※ Provided by Dr. Masahiro Kamita, Ph.D, National Cancer Center Research Institute, JAPAN.

4. Protein Adsorption to vessel

Diluted enzyme-labeled antibody solutions from 0.1-0.8 mg/mL with phosphate buffer, and dispensed diluted the solutions into non-coated tube, competitive tube and ProteoSave™. After 1 h incubation at 37°C, washed by phosphate buffer, then the amount of adsorbed enzyme-labeled antibodies was measured by microplate reader.

5. Chemical, Heat and Cold Resistant

Organic solvent 10%, 5hr Methanol ✔ Ethanol ✔ 2-propanol ✔ Glycerol ✔ Acetonitrile ✔ Acetone ✔ DMSO ✔ 2-mercaptoethanol ✔

Surfactant 0.1% CHAPS ✔ Triton X-100 ✔ Tween 20 ✔ SDS ✔

Heat resistant Cold resistant 100 °C (10 min) -80°C (3 months)

 

6. Site lists

• Publication list
• Sumitomo Bakelite Website

7. Product lists

Centrifuge tube 15 mL & 50 mL	Micro tube 0.5 mL & 1.5 mL	Slim tube 0.5 mL & 1.5 mL	96-deep wells plate	96-wells flat & U bottom plate

 

Wako Cat No. S-Bio Cat No.	Product Name	Material	Note	Qty/ Pk	Qty/ Cs
638-27509 MS-4205MZ	ProteoSave™ 0.5 mL Microtube	Polypropylene	Non-sterilized	100	500
632-27529 MS-4255MZ	ProteoSave™ 0.5 mL Microtube (Sterilized)	Polypropylene	Radiation sterilized	100	500
635-27519 MS-4215MZ	ProteoSave™ 1.5 mL Microtube	Polypropylene	Non-sterilized	100	500
639-27539 MS-4265MZ	ProteoSave™ 1.5 mL Microtube (Sterilized)	Polypropylene	Radiation sterilized	100	500
MS-4220MZ	ProteoSave™ 2 mL Microtube	Polypropylene	Non-sterilized	100	500
MS-4270MZ	ProteoSave™ 2 mL Microtube (Sterilized)	Polypropylene	Radiation sterilized	100	500
638-27489 MS-4201XZ	ProteoSave™ 0.5 mL Slimtube	Polypropylene	Non-sterilized	50	500
635-27499 MS-4202XZ	ProteoSave™ 2 mL Slimtube	Polypropylene	Non-sterilized	50	500
630-28549 MS-8296FZ	ProteoSave™ 96-wells Flat Plate	Polystyrene	Non-sterilized	5	50
MS-8296KZ	ProteoSave™ 96-wells Black, Flat Plate (Made to order)	Polystyrene	Non-sterilized	5	50
639-29599 MS-8296VZ	ProteoSave™ 96-wells V Plate	Polypropylene	Non-sterilized	5	20
MS-82962Z	ProteoSave™ 96-deep wells V Plate, 2mL	Polypropylene	Non-sterilized	3	45
639-27419 MS-3296UZ	ProteoSave™ 96-wells U Plate	Polystyrene	Non-sterilized	5	50
638-28109 MS-52150Z	ProteoSave™ 15 mL Conical Tube	PET	Non-sterilized	5	100
635-28119 MS-52550Z	ProteoSave™ 50 mL Conical Tube (Sterilized)	Polypropylene	Radiation sterilized	5	100