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Coating agent of cell culture vessels for ES cells/iPS cells
StemSure Gelatin Solution

This product is a coating reagent for cell culture vessels including dishes and multiwell plates.
It uses gelatin derived from pork skins and is prepared with D-PBS(-).



INDEX

  1. Features
  2. Test items
  3. Culture of human iPS cell line 201B7 (on feeder cells)
    • Cell morphology and ALP staining
  4. Culture of murine ES cell line D3 (feeder-free)
    • Cloning efficiency
    • Identification of expression of markers for undifferentiated cells
    • Identification of teratoma formation
  5. Product list
  6. Related products

  7. Reagents for ES·iPS Cell Culture 2013




Image



1. Features

  • Quality test is conducted for each lot using murine ES cell line D3.
  • Already adjusted to the concentration of use, thus directly applicable to coating of cell culture vessels.


2. Test items

  • Colony formation assay (in murine ES cell line D3)
  • Alkaline phosphatase assay(in murine ES cell line D3)
  • Sterility test
  • Endotoxin
  • Mycoplasma testing


3. Culture of human iPS cell line 201B7 (on feeder cells)

<Colony morphology and ALP staining>

Multiwell plate was coated with StemSure gelatin solution. After the coating, feeder cells were spread and human iPS cell line 201B7 were cultured on the feeder cells. Then the cell morphology and positive ALP staining were identified.

Image D-MEM/Ham's F-12 + 20% SSR + 2 mmol/L L-Glutamine + 1 x MEM Non-essential
Amino Acids + 0.1 mmol/L StemSure 2-Mercaptoethanol +
1 x Penicillin-Streptomycin + 5 ng/mL bFGF

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4. Culture of murine ES cell line D3 (feeder-free)

<Cloning efficiency>

Murine ES cell line D3 was cultured on a multiwell plate coated with either StemSure gelatin solution or a similar product of company A and the cloning efficiency was compared. Image


<Identification of the expression of markers for undifferentiated cells>

Murine ES cell line D3 was subcultured 16 times in a multiwell plate coated with StemSure gelatin solution and several kinds of undifferentiated markers (Oct3/4, Sox2, Nanog) were identified as positive. Image


<Identification of teratoma formation>

Murine ES cell line D3 was cultured in a multiwell plate coated with StemSure gelatin solution. The cells were subcutaneously injected into an immunodeficient mouse. Teratomas were subcutaneously formed and the nerve tissue (derived from ectoderm), cartilage tissue (derived from mesoderm), and lumenal structures with ciliated epithelium (derived from endoderm) were identified in them Image
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5. Product list

Product Name Grade Package Size Wako Cat. No.
StemSure 0.1 w/v% Gelatin Solution for Cell culture 500 mL 190-15805

6. Related products

★StemSure series★ <for Cell Culture>

StemSure series are the products available for culture of ES cells or iPS cells. Quality test is conducted for each lot using murine ES cell line D3.

Product Name Package Size Wako Cat. No. Storage condition
StemSure D-MEM (High Glucose) with Phenol Red and Sodium Pyruvate 500 mL 197-16275 Keep at 2-10°C.
StemSure Serum Replacement (SSR) 500 mL 197-16775 Keep at -20°C.
StemSure 10 mmol/L 2-Mercaptoethanol Solution (x 100) 100 mL 198-15781
StemSure 50 mmol/L Monothioglycerol Solution (x 100)
A reducing agent equivalently available as 2ME. Not applicable topoisonous substance
100 mL 195-15791
StemSure LIF, Mouse, recombinant, Solution 106 units 199-16071
StemSure Freezing Medium     ⇒ Not available for sales in the US. 100 mL 195-16031
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★Liquid medium/reagents for cell culture★

We have prepared various kinds of products including liquid medium, balanced salt solution, solution for cell ablation/dispersion, antibiotic solution, and extracellular matrix.
LINK


★Reagents for ES/iPS cell research★

A number of documents about iPS cells have been published since announcement of establishment of iPS cells in 2007. We have a line-up of low molecular weight compound, which have been reported to be concerned with maintenance of undifferentiation potency as well as differentiation induction of the ES cells/iPS cells in many publications.
Reagents for ES·iPS Cell Culture 2013


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Note
  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
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