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New Liposome developed by click chemistry
ScreenFect™ A plus < DNA & siRNA Transfection Reagent >

ScreenFect™ A plus is a transfection reagent composed of a new cationic loposome screened*1 by click chemistry.
It can be used with various eukaryote-derive cells and can be directly added to medium containing antibodies or serum.

DNA and siRNA can be transfected into general experimental cell strains (HeLa, HepG2, MECK, etc.), stem cells (mouse ES cells, etc.), blood cells (macrophages, THP-1, RAW264, 7, etc.), microglia, and primary (initial subculture) cells. Medium replacement after transfection is not required due to low cytotoxicity. The constiuent reagents do not contain any poisonous or deleterious substance
*1: Biomaterials. Nov., 33 (32): 8160‐6. 2012


ScreenFect™ was
advertised in nature
on Nov. 27, 2014. ⇒ zoom


1. Features

  1. Broad Ranges of [DNA : Reagents ] Mix Ratio
  2. One-step Transfection
  3. High Transfection Efficiency and Low Toxicity
  4. Low Running Cost



2. ScreenFect™ A plus Transfection performance (Flow Cytometry)

(1) MCF-7 (Human breast cancer (adherent)
* MCF-7:   Human breast cancer (adherent)
* Protocol: A company A's product → 2-Step
    ScreenFect™ A plus → 1 Step
High transfection performance for lipofection of difficult cells!

(2) MDCK: Madin-Darby canine kidney (adherent)
* MDCK: Madin-Darby canine kidney (adherent)
* Protocol:
    A company A's new product (+ reagent)
                                        → 2-Step
    ScreenFect™ A plus → 1 Step
High transfection performance for lipofection of difficult cells!


(3) K562: Human erythromyeloblastoid leukemia (suspension)
* K562: Human erythromyeloblastoid leukemia (suspension)
* Protocol:
  A company A's product
    & the new product (+ reagent)
                                    → 2-Step

 ScreenFect™ A plus → 1 Step
High transfection performance for lipofection of difficult suspension cells!
3. 1-Step vs 2-Step ⇒ Standard Protocol of siRNA transfection (/well)
Product ScreenFect™ A plus Company A's new product
(+ reagent)
Company A's product
  Recommended Protocol
1-Step
2-Step
  Experiment term
2 Days
3 Days
  DNA quantity
Low
High
  Adjustment of cell numbers
Flexible
(cell preparation just before transfection)
Inflexible
(depending on cell pre-culture condition)
  Detachment by trypsin
Required
(just before transfection)
Required
(before cell pre-culture)
  Convenience for
  HTS cell-base assay
+++++
+
  Medium change
Depending on cell lines
4. ScreenFect™ A plus 1-Step Protocol




The 1-Step Protocol is 24 hours faster than 2-Step's
5. ScreenFect™ A plus Lipoplex Preparation
DNA Transfection
DNA transfection (/well)
Plate size Surface area Medium volume Total volume
SF-DNA complex
DNA
/Dilution Buffer
ScreenFect™ A plus
/Dilution Buffer
96 wells
0.3 cm2
80 μL
20 μL
~ 100 ng / 10 μL
~ 0.4 μL/10 μL
24 wells
2 cm2
350 μL
80 μL
~ 500 ng / 40 μL
~ 2.0 μL/40 μL
12 wells
4 cm2
700 μL
140 μL
~ 1,000 ng / 70 μL
~ 4.0 μL/70 μL
6 wells
10 cm2
1,250 μL
240 μL
~ 2,500 ng / 120 μL
~ 10 μL/120 μL
Note: For large scale transfections it helps to split sample between several tubes. For example, take 5-6 samples of the 6-well transfection volumes for 10 cm dish transfection, .
ScreenFect™ A plus recommended protocol
The one-step protocol for ScreenFect™ A plus transfection is described in the upper part. The optimal DNA-to-reagent-ratio for efficient cell transfection stays constant at around 1 : 3 to 1 : 4 ( DNA 100 ng : ScreenFect™ A plus reagent 0.1 μL = 1 : 1 ) and we recommend splitting cells when they reach 60% ~ 80% confluence to avoid contact inhibition of cell proliferation.

Please visit our website for easy protocol for transfection http://screenfect.jp.
Wako will provide a free-sample for your evaluation. Please contact us .

siRNA Transfection
siRNA transfection (/well)
Plate size Surface area Medium volume Total volume
SF-DNA complex
siRNA
/Dilution Buffer
ScreenFect™ A plus
/Dilution Buffer
96 wells
0.3 cm2
80 μL
20 μL
2 ~ 3 pmol /10 μL
0.1 ~ 0.3 μL/10 μL
24 wells
2 cm2
350 μL
80 μL
10 ~ 20 pmol / 40μL
0.5.~ 1.5 μL/40 μL
12 wells
4 cm2
700 μL
140 μL
20 ~ 40 pmol / 70μL
1.0 ~ 3 μL/70 μL
6 wells
10 cm2
1,250 μL
240 μL
20 ~ 60 pmol / 120μL
3.5 ~ 7.5 μL/ 120 μL
Note:

Although ScreenFect™ A plus works well for siRNA transfection, we highly recommend the use of our specialized reagent ScreenFect™ siRNA. Please visit the page.

6. Product List
Product Name ScreenFect™ A plus
Wako Catalog No. 293-77101 299-77103 297-77104
Packge Size 0.2 mL 1 mL 1 mL x 5
 Kit Contents
(1) Transfection Reagent
0.2 mL
1 mL
1 mL x 5
(2) Dilution Buffer
10 mL
50 mL
50 mL x 5
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