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New Liposome developed by click chemistry
ScreenFect™ mRNA
mRNA & Long RNA transfection reagents


ScreenFect™ mRNA is transfection reagent which includes noel cationic liposome derived from click chemistry screening system*1. ScreenFect™ mRNA is suitable for transfection into eukaryotic cells (HeLa, HEK293, MDF cells, etc.) without exchanging of culture medium because it is not included hazard compounds. ScreenFect™ mRNA is used for mRNA transfection procedures.
*1: Biomaterials, Nov.; 33(32): 8160-76, 2012.




1. Features


・Direct transfection of mRNA

・Long RNA transfection

・iPS cell line established without virus vector


2. GFP mRNA transfection into HEK293 cells


GFP mRNA transfection into HEK293 cells


Transfection condition using ScreenFect™ mRNA
  • Cell line: HEK293
  • Protocol: One-step protocol (See below), no-medium replacement
  • Plate size: 96 well plate
  • Volume of transfection reagent: 0.3 μL/well
  • RNA amount: GFP mRNA [TriLink #L-6101] 75 ng/well
  • Detection time: 24 hours after the transfection
  • Detector: Cytation3 microscope [BioTek]



3. Transfection condition with ScreenFect™ mRNA


  96-well 24-well 12-well 6-well
Surface area
0.35 cm2
1.9 cm2
3.8 cm2
9.6 cm2
Cell number
0.05 - 0.2 x 105
0.5 - 1 x 105
1 - 2 x 105
2 - 5 x 105
ScreenFect™mRNA Transfection
Reagent / Dilution Buffer
0.15 - 0.4 µL
/ 8 µL
0.75 - 2 µL
/ 40 µL
1.5 - 4 µL
/ 75 µL
3 - 6 µL
/ 120 µL
mRNA/ Dilution Buffer
0.050 - 0.25 μg
/ 8 µL
0.25 - 1.25 μg
/ 40 µL
1 - 2.4 μg
/ 75 µL
1.25 - 6.2 μg
/ 120 µL
Lipoplex (DNA/lipid complex) amount
16 µL
80 µL
150 µL
240 µL
Cell suspension
84 µL
420 µL
750 µL
1260 µL
Final volume
100 µL
500 µL
900 µL
1500 µL

4. mRNA transfection protocol



 

5. Product List


mRNA transfection protocol

Product Name Kit Contents Pkg. Size Wako Cat. No. Storage Condition
ScreenFect™ mRNA
Transfection Reagent
Dilution Buffer
for ScreenFect™ mRNA
ScreenFect™ mRNA
0.2 mL
10 mL
0.2 mL
Keep at 2 - 10°C
1 mL
50 mL
1 mL
1 mL x 5
50 mL x 5
1 mL x 5


6. Related Products link to Nippon Gene


[Complete RNA series]
The following products are homogeneous mRNAs. Cap structure at the 5' end and a Poly(A) tail at the 3' end are added to the RNA full-length sequence, respectively.
They can be used as templates to obtain control data of absolute quantitation or validation in real-time PCR assay .

Product Name Pkg. Size Wako Cat. No. Storage Condition
Albumin mRNA, Human
100 ng
Keep at -80°C
Beta Actin mRNA, Human
100 ng
GAPDH mRNA, Human
100 ng
Beta Actin mRNA, Rat
100 ng
Cebpd mRNA, Rat
100 ng
Ctss mRNA, Rat
100 ng
GAPDH mRNA, Rat
100 ng
Hsd17b2 mRNA, Rat
100 ng
Hpx mRNA, Rat
100 ng
Lgals9 mRNA, Rat
100 ng
Mt1a mRNA, Rat
100 ng
Orm1 mRNA, Rat
100 ng

*For the latest price information, access Wako Online Catalog with a click of each Wako Catalog No.


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