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New Liposome developed by click chemistry
ScreenFect™ siRNA < DNA & siRNA Transfection Reagent >

ScreenFect™ siRNA is a transfection reagent composed of new cationic lipids selected by screening experiments *1 by click chemistry. It is applicable to cell lines from various eukaryotic species and can be directly added to medium containing serum.
Because of the low cell toxicity, medium change after transfection is not required. Furthermore, this kit does not contain any poisonous and deleterious components.
*1: Biomaterials. Nov., 33 (32): 8160‐6. 2012

ScreenFect™ was advertised in nature
on Nov. 27, 2014. ⇒ zoom


1. Features

  1. Highly knockdown efficiency & Low cell toxicity
  2. Provided Buffer optimized siRNA transfection
  3. Requires no medium change after transfection
  4. Serum Compatible



2. Protocol
⇒ siRNA Transfection including cell detachment procedure (24 wells)
∗2:
ScreenFect™ siRNA Dilution Buffer is a Wako-recommended buffer for transfection. However, basal medium such as D-MEM, E-MEM may be more suitable depending on cells. Please optimize dilution condition for your cells.

∗3:
Add ScreenFect™ siRNA Transfection Reagent into Dilution Buffer with rapid pipetting (4 - 5 times). Tap the tube with finger for well-mixing if necessary.

∗4:
This one-step method is applicable to not all cell types. When you does not get ideal transfection efficiency at the protocol, remove the medium from cells, first. Then add 420 μL of new medium to SF-siRNA complexes, mix by pipetting, and immediately apply to the cells.



⇒ siRNA Transfection without cell detachment procedure (24 wells)
∗2:
ScreenFect™ siRNA Dilution Buffer is a Wako-recommended buffer for transfection. However, basal medium such as D-MEM, E-MEM may be more suitable depending on cells. Please optimize dilution condition for your cells.

∗3:
Add ScreenFect™ siRNA Transfection Reagent into Dilution Buffer with rapid pipetting (4 - 5 times). Tap the tube with finger for well-mixing if necessary.

∗5:
This one‐step method is applicable to not all cell types. When you does not get ideal transfection efficiency at the protocol, remove the medium from cells, first. Then add 420 - 500 μL of new medium to SF-siRNA complexes, mix by pipetting, and immediately apply to the cells.

∗6: RT: room temperature
Notice
  • First, dilute ScreenFect™ siRNA Transfection Reagent and siRNA with Dilution Buffer provided, respectively as mentioned above.
  • Since the siRNA transfection efficiency depends heavily on cell type, the condition, the passage number and combination with siRNA transfected, please adjust the each concentration of ScreenFect™ siRNA Transfection Reagent and siRNA, respectively before starting on new transfection experiment.
    Please refer the package insert for detailed protocol.
  • For additional information and protocols on transfection please visit Wako website.

⇒ Standard Protocol of siRNA transfection (/well)



3. Applications: ScreenFect™ siRNA
⇒ Knock-down efficiency



LRP6 siRNA was transfected into MEF. LRP expressions after 24 hours were examined by Western blotting. Knockdown effect of ScreenFect™ siRNA is the same or more than Competitor A and B.

Cell Strain: Mouse Embryonic Fibroblast (MEF)
Plate Size: 24 wells

siRNA Target: LRP6
  ScreenFect™ siRNA transfection condition:
  siRNA (LRP6): 1 pmol
  Transfection Reagent: 0.1 μL
  Suspended Cells: 420 μL



⇒Cytotoxicity


Verification of cytotoxicity to HEK293 cells after 24 hours of transfection by fluorescence microscope The cytotoxicity of ScreenFect™ siRNA is lower than that of the competitor A’s product.

Cell strain: HEK293 cells
Plate Size: 96 wells
Staining: Hoechst stain







Wako is regularly updating the application data of ScreenFect™ A. Please contact us about the applicability of your cell strains.

4. Related Product: ScreenFect™ A (Broad Range Transection)
ScreenFect™ A is a transfection reagent composed of new cationic lipids selected by screening experiments by click chemistry. It is applicable to cell lines from various eukaryotic species and can be directly added to medium containing antibiotic and serum. ScreenFect™ A works with DNA and siRNA and can show highly efficient transfection in common cell lines (HeLa, HepG2, MDCK, Cos-7, etc.) stem cells (mouse embryonic stem cells, etc.), blood cells (macrophage, THP-1, RAW264.7, etc.), microglia, primary culture cells. ⇒ LINK to




5. Product List
Product Name ScreenFect™ siRNA
Wako Catalog No. 299-75001 295-75003 293-75004
Packge Size 0.2 mL 1 mL 1 mL x 5
Kit Contents
(1) Transfection Reagent
0.2 mL
1 mL
1 mL x 5
(2) Dilution Buffer
10 mL
50 mL
50 mL x 5
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