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For selection of transformed cells_Selection Antibiotic Solutions
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⇒ Leaflet (1.1 MB/2P)   
Blasticidin S Hydrochloride


Blasticidin S hydrochloride is a nucleoside antibiotic which inhibits protein synthesis in both prokaryotes and eukaryotes. The resistance is conferred by expression of either BSD or bsr, Blasticidin S deaminase genes that convert Blasticidin S to a non-toxic deaminohydroxy derivatives.
Blasticidin S hydrochloride is used to select transfected cells carrying BSD or bsr resistance genes.



CAS No. 3513-03-9
C17H26N8O5·HCl = 258.90

[Product Outline]

  • Source: Streptomyces griseochromogenes
  • Resistance gene: BSD (from Aspergillus terreus
    bsr (from Bacillus cereus)
  • Appearance:
        Blasticidin S Hydrochloride: Powder
        HEPES Solution: 10 mg/mL Blasticidin S in 20 mmol/L HEPES (pH 7.5)
[Working concentration]

  • Selection in E. coli: 50 - 100 μg/mL
  • Selection in mammalian cell lines: 2 - 10 μg/mL
Product Name Package Size Wako Cat. No. Grade Storage condition
Blasticidin S Hydrochloride 100 mg 029-18701 for Genetic Research Keep at 2 - 10°C
10 mg/mL Blasticidin S Hydrochloride, HEPES Solution 1 mL
1 mL x 10
026-18711
022-18713
Keep at - 20°C


QUOTATION REQUEST for Selection Antibiotics

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G418 Sulfate Solution

G418 is an aminoglycoside antibiotic similar in structure to gentamicin. It inhibits protein synthesis.
At high sconcentrations (0.1 ~ 1 mg/mL), it inhibits cell growth of yeast, fungi and higher animals
and plants as well as prokaryote.
This product is ready-to-use 50 mg/mL G418 Sulfate Solution.




LINK to G418 homepage
G418 Sulfate Solution was advertised in nature
on Oct. 30, 2104. ⇒ zoom


[Product Outline]

  • Resistance gene: neor
    (encoding aminoglycoside 3-phosphotransferase (APH3 ))
  • Concentration: 50 mg/mL (as G418) in sterile water
  • pH: 5.0 - 6.0:
[Features]

  • Ready-to-use solution
  • Sterilized by filter
    Sterility, Mycoplasma, and bacterial endotoxin test were conducted.
  • Animal-derived-free solution
[Working concentration]

  • The optimal concentration for resistant clones selection in mammalian cells depends on the cell line used as well as on the plasmid carrying the resistant genes.
  • Please adjust the optimal concentration every experimental system.




  • CAS No. 108321-42-2
    C20H40N4O10·2H2SO4 = 692.71
      
    Product Name Package Size Wako Cat. No. Grade Storage condition
    50 mg/mL G-418 Sulfate Solution, Animal-derived-free 20 mL
    100 mL
    071-06431
    077-06433
    for Genetic Research Keep at 2 - 10°C



    Product List
    Product Name Package
    Insert
    Package Size Wako Cat. No. Grade Storage condition
    Ampicillin
      Ampicillin Solution - 5 g
    10 g
    25 g
    016-23301
    012-23303
    014-23302
    for Biochemistry Keep at 2 - 10°C
    Blasticidin S Hydrochloride
      Blasticidin S Hydrochloride (91KB)
    100 mg 029-18701 for Genetic Research Keep at 2 - 10°C
      10 mg/mL Blasticidin S Hydrochloride, HEPES Solution (94KB)
    1 mL
    1 mL x 10
    026-18711
    022-18713
    Keep at - 20°C
    G418
      G418 Sulfate - 1 g
    5 g
    25 g
    100 g
    078-05961
    074-05963
    076-05962
    072-05964
    for Genetic Research Keep at 2 - 10°C
      50 mg/mL G418 Sulfate Solution, Animal-derived-free (95KB)
    20 mL
    100 mL
    071-06431
    077-06433
    Geneticin®
      50 mg/mL Geneticin® Solution - 20 mL
    100 mL
    075-04893
    079-04891
    for Genetic Research Keep at 2 - 10°C
    Hygromycin B
      Hygromycin B (99KB)
    1 g
    5 g
    25 g
    089-06151
    085-06153
    087-06152
    for Biochemistry Keep at 2 - 10°C
      50 mg/mL Hygromycin B Solution - 20 mL
    100 mL
    084-07681
    080-07683
    Kanamycin Sulfate
      Kanamycin Sulfate - 1 g
    5 g
    100 g
    113-00701
    119-00703
    117-00704
    for Cell Culture Keep at 2 - 10°C
      Kanamycin Sulfate Solution - 20 mL 117-00961
    Puromycin Dihydrochloride
      Puromycin Diydrochloride - 25 mg
    100 mg
    250 mg
    160-23151
    166-23153
    164-23154
    for Biochemistry Keep at - 20°C





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