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Next Generation Affinity Tag System from Japan - Recombinant protein productions from Animal cells
TARGET tag



TARGET tag system is an affinity purification system developed in Japan using a monoclonal antibody against the peptide tag.
It has been reported in scientific papers that they have succeeded to rapidly express and purify recombinant proteins which are difficult to express in animal cells by applying the TARGET tag.
An animal cell expression system requires an affinity tag for efficient purification due to low recombinant protein recovery.
TARGET tag system has overcome shortcomings of the traditional commercial affinity purification systems such as high cost and long time-consuming.




  INDEX

  1. TARGET tag System
  2. TARGET tag Expression Vectors
  3. Anti TARGET tag Monoclonal Antibody (P20.1)
  4. Applications
  5. Product List
  6. Leaflet Link to Leaflet

  ⇒  LINK to





1. TARGET tag System





2. TARGET tag Expression Vectors




Product Selection
Vector Expression pattern Host MCS promoter
pEBMulti Stable episomal expression Primate animal cells (humans, monkeys, dogs CAG
pCAG Transient expression Animal cells (mice, rats), in addition to the above CAG


We also offer a lineup of products of other vectors with antibiotic resistance genes different from the above (please see p.4 of the leaflet).
Detailed information on the sequencing primers and other vector sequences are included in the instruction manual supplied with the product. The sequence information (compressed file) can be downloaded from our website.


3. Anti TARGET tag Monoclonal Antibody (P20.1)

[Concentration]
  Indicated on the label.
  (Approx. 1.0 mg/mL)

[Formulation]
  1 x PBS (pH 7.5) aqueous solution.

[Clone No.]
  P20.1

[Subclass]
  IgG1

[Working Dilution]
  Western Blot 0.1-1 μg/ml
  Immunoprecipitation 10-50 μg/assay

  Please optimize the most appropriate concentration for your analysis conditions
  and samples.

[Storage]
  Keep at -20°C.
  Avoid repeated freezing and thawing.

4. Applications






The expression level and detection sensitivity were proved to be equivalent to those of the traditional tag system.



Immunoprecipitation (IP) (2 hr, 4°C) was performed with 0.1 mL (net 0.05 mL) of the antibody-conjugated beads ([1] anti-TARGET and [2] anti-DYKDDDDK tag antibodies) per 10 mL of culture media supernatants (after 7-day culturing). After washing the beads, 0.2 mg/mL of TARGET tag peptide/TBS or DYKDDDDK peptide/TBS was added and incubated (0.5 hr, 4°C) with gentle agitation. The supernatant was collected after centrifugation, and the beads were incubated (5 min, RT) with gentle agitation after adding 0.5 mL of 0.1M Glycine-HCl (pH 2.7). After centrifugation, the supernatant was neutralized with 1M Tris-HCl (pH 9.0). Figure (above): Silver staining after SDS-PAGE
Lower background compared to the traditional DYKDDDDK tag



Can be used in commercial transient vectors

5. Product List


Product Selection
Vector Expression pattern Host MCS promoter
pEBMulti Stable episomal expression Primate animal cells (humans, monkeys, dogs) CAG
pCAG Transient expression Animal cells (mice, rats), in addition to the above CAG

Product Name Selection Marker Package Size Wako Cat. No. Storage Condition
E. coli Animal Cells
pCAG-Ble TARGET tag-C Bleomycin 20 μg 165-26381 Keep at -20℃,
pCAG-Ble TARGET tag-N Bleomycin 20 μg 162-26391
pCAG-Bsd TARGET tag-C Blasticidin S 20 μg 165-26401
pCAG-Bsd TARGET tag-N Blasticidin S 20 μg 162-26411
pCAG-Hyg TARGET tag-C Hygromycin B 20 μg 169-26421
pCAG-Hyg TARGET tag-N Hygromycin B 20 μg 166-26431
pCAG-Neo TARGET tag-C Kanamycin G418 20 μg 163-26441
pCAG-Neo TARGET tag-N Kanamycin G418 20 μg 160-26451
pEBMulti-Ble TARGET tag-C Bleomycin 20 μg 167-26461
pEBMulti-Ble TARGET tag-N Bleomycin 20 μg 164-26471
pEBMulti-Bsd TARGET tag-C Blasticidin S 20 μg 161-26481
pEBMulti-Bsd TARGET tag-N Blasticidin S 20 μg 168-26491
pEBMulti-Hyg TARGET tag-C Hygromycin B 20 μg 161-26501
pEBMulti-Hyg TARGET tag-N Hygromycin B 20 μg 168-26511
pEBMulti-Neo TARGET tag-C Kanamycin G418 20 μg 165-26521
pEBMulti-Neo TARGET tag-N Kanamycin G418 20 μg 162-26531
pEBMulti-Puro TARGET tag-C Ampicillin Puromycin 20 μg 169-26541
pEBMulti-Puro TARGET tag-N Ampicillin Puromycin 20 μg 166-26551

About the license: When purchased, please use the product for research purposes only. Please contact our customer service if you intend to use the product for manufacturing and marketing of recombinant proteins or cell strains, such as reselling of the product, or selling plasmids that are newly constructed using the DNA sequence information or DNA fragments derived from the product.



Product Name Package Size Wako Cat. No. Storage Condition
Anti TARGET tag, Monoclonal Antibody (P20.1) 200 μg 016-25481 Keep at -20°C.
1 mg 012-25483
Anti TARGET tag, Monoclonal Antibody (P20.1), Peroxidase conjugated 200 μL 015-25571
1 mL 011-25573
Anti TARGET tag Antibody beads 2 mL 018-25561 Keep at 2 - 10°C.
10 mL 014-25563
50 mL 012-25564
TARGET tag peptide 5 mg 200-19673 Keep at -20°C.
25 mg 208-19674
TARGET tag Washing Solution 50 mL 208-19831


Reagents described herein are intended to be used for experimental or research purposes only.
They cannot be used as "medical supplies", "food" or "household goods". ˜ For the latest information, please refer to the Wako online catalog (http://www.e-reagent.com/).
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