<Tamavidin™2-REV ~ reversible biotin-binding capability ~>
Takakura, Y. et al.: "Tamavidin 2-REV : an engineered tamavidin with reversible biotin-binding capability.", J. Biotechnol., 164(1), 19-25(2013).
Tamavidin™2-REV is an engineered protein from Tamavidin™2, the novel avidin-like protein. Tamavidin™2-REV has the capability of the reversible biotin-binding, and can be eluted the biotinylated molecules by the excess amount of biotin under mild condition.
[Under the normal condition, the binding of avidin/streptavidin and biotin is irreversible.]
- 1. Outline Tamavidin™2 Magnetic Beads
- 2. Application Data
- 3. Products outline of Tamavidin™2 Series
- 4. LINK
Recovery test of biotinylated DYKDDDDK-BAP
Immunoprecipitation by the biotinylated antibody
RNA Pull-down Assay (HOTAIR RNA)
MagCapture™ Tamavidin™2-REV is the slurry of Tamavidin™ 2-REV immobilization magnetic beads.
Tamavidin™ 2-REV is a genetic mutant of Tamavidin™2 which is identified as a novel avidin-like protein from mushroom. Tamavidin™2-REV is a useful tool for the purification of biotinylated molecules such as nucleic acids, chemicals, antibodies and enzymes by using competitive biotin elution methods.
Recombinant Tamavidin™ 2 Series
*1 Isoelectric Point: The value of Tamavidin™2-LPI only is the actual measured value. Others are calculated from the amino acid sequence.
Wako Pure Chemical Industries, Ltd. has sold the series of Tamavidin™ under the sales license from Japan Tobacco Inc..