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A Novel Thermostable Avidin-like Biotin-Binding Protein
Tamavidin 2, recombinant

Tamavidin 2 is a soluble recombinant avidin which is purified from E. coli. expressed mushroom (Pleurotus cornucopiae) Tamavidin 2 cDNA.
Tamavidin 2 is used for immunological assay by using biotinylated chemicals, antibodies and enzymes.

[Features]

  • Higher Thermostable
  • Lower Background than using conventional avidin
  • Applicable for Immunological Assay
  • Leaflet (1.1MB/2 p)

    [LINK]

  • RNA2015 Technical seminar ~iSRIM ( In Vitro Specificity based RNA regulatory protein Identification Method ) and Tamavidin™ 2-REV Magnetic Beads~
  •       
    Tamogitake Mushroom
    Tamogitake mushroom (Pleurotus cornucopiae)


    alt here...
    RNA2015 Technical seminar

    Lower Backgrpound than using Egg Avidin!!!
    Lower Background than Egg Avidin!!!

    The non-specific background of Tamavidin 2 coated plate is lower than
    Avidin from Egg coated plate in the case of addition of human serum
    in plate wells.    (t-test: *p< 0.001, n = 8)

     

          
    Biotin Binding Activity
     
    ka
    (M-1s-1)
    kd
    (s-1)
    kd
    (M)
    Biotin labeled BSA
      1.0 x 106  
    ND
    -
    Iminobiotin labeled BSA
      1.0 x 104   
      8.7 x 10-4  
      8.7 x 10-8
    (Assay result by BIAcore)

    [Source] E. coli expressed Pleurotus cornucopiae Tamavidin 2

    [Activity] Indicated on each label
                     (5 ~ 20 Units/mg (HABA method at pH 5)

    [Molecular Weight] Approximately 60 kDa (Tetramer)

    [Thermostability] Activity: Tm = 85 °C
       (Tm = temperature of half-life period of biotin binding activity
         after heat treatment.)

     


    [Activity of Thioredoxin in cells]

    Avidin or Avidin-like protein
    Tm
    Tamavidin 2
    85 °C
    Streptavidin
    74 °C
    Avidin from Egg
    79 °C
    References
    1. Takakurak, Y., et al.: "Tamavidins-novel avidin-like biotin-binding proteins from the Tamogitake mushroom", FEBS J., 276 (5), 1383-97 (2009)
    2. Takakura, Y., et al.: "Tamavidin, a versatile affinity tag for protein purification and immobilization", J. Biotechnol., 145 (4), 317-22 (2010)
            

    Tamavidin 2 SDS-PAGE

     

    Tamavidin 2 is detected at 15 kDa (main band) and 30 kDa (miner band) by SDS-PAGE

    Tamavidin 2 is tetramer which is constructed from four subunits (15.5 kDa/subunit)

    This subunit includes one biotin binding domain.

    SDS-PAGE of Tamavidin 2

    License
    Tamavidin is registered as a trademark of Japan Tobacco inc. in Japan.


    Product List
    Description Wako Catalog No. Package Size Storage Condition
    Tamavidin 2, recombinant
    209-18261
    1 mg
    -20ºC
    205-18263
    5 mg
    203-18264
    25 mg

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    Products listed in this page are for research use only.  Do not administer each to human.
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