For target mRNA research
Target mRNA Cloning Kit Wako
(Wako catalog #298-68001)
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Target mRNA Cloning Kit Wako is used for the cDNA amplification of low
population mRNA. Especially, this product will be used for the cloning of
microRNA-targeted mRNA from Ago IP RNA fraction. The amplified cDNA
will be target mRNA of microRNA.
Features
Simple Protocol
Amplification of low population mRNA
Independent cDNA amplification to the length of mRNAs
Investigation of microRNA targeted mRNA
Outline of cDNA amplification of target mRNA
Product information
Code No.
Description
Size
298-68001
Target mRNA Cloning Kit Wako
10 reactions
291-70201
microRNA Isolation Kit, Human/Mouse Ago1
10 reactions
292-66701
microRNA Isolation Kit, Human Ago2
10 reactions
292-67301
microRNA Isolation Kit, Mouse Ago2
10 reactions
297-70301
microRNA Isolation Kit, Human Ago3
10 reactions
298-67901
PCR Purification Kit Wako
30 reactions
Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.
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Application(Investigation of miR-122 targeted mRNA)
Outline of Experiment
① Electrophoresis of the amplified cDNA
② Cloning of the amplified cDNA
③ Target mRNA validation (qRT-PCR)
cDNA clone harboring target sequences of miR-122
Quantification of mRNA(CSP*1 of Clone ≥ 80)
Context Score Percentile(CSP*1)
> 80
< 80
cDNA clones
PIGS, BRPF1, SLC7A5,
PPP1R9A, SLC1A5,
ALDOA, OBFS2B etc…
TACC1,
DKF7p761F198, NMF6,
FLJ14397, MRP63,
SH3GL1, DIS3 etc…
*1 CSP:Context Score Percentile
The value of reasonability parameter as a target mRNA.
(TargetScan)
*2 Control cells
The HepG2 cells which were transfected GL3(Luciferase)
siRNA.
Published Application !!
Hayashida, Y., et al. : A useful approach to total analysis of
RISC-associated RNA., BMC Research Notes, 2, 169(2009)
ALDOA mRNA was already known as a target of miR-122.
Additionally, several mRNA were screened as new potential target mRNA of miR-122 in HepG2 cells.