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Home > Products > Life Science >  Tau ELISA Kit Wako

For Alzheimer’s disease research
Tau ELISA Kit Wako

Tau is a microtubule-associated protein that is mainly expressed in neurons in the central nervous system and regulates stability of microtubules. In the brain of patients with Alzheimer’s disease, the aggregates of phosphorylated Tau or neurofibrillary tangles is formed, and the extent of formation has been reported to correlate with severity of dementia. Therefore, Tau has been studied to investigate causes of Alzheimer’s disease and to develop drugs for treating the disease. As the concentration of Tau and phosphorylated Tau in cerebrospinal fluid (CSF) of Alzheimer’s disease patients is higher than that of non-dementia patients, it is important to measure the concentration of Tau and phosphorylated Tau in CSF. This ELISA is high sensitive kit to measure Tau including phosphorylated Tau in CSF.


1. LINK

2. Features

✔ Small sample volume 10μL~
✔ High sensitivity 4.10~1000 pg/mL
✔ Detects differences between dementia stages

3. Components

1. Antibody-coated Plate 1 Plate
2. Tau Standard 1 Tube
3. Buffer 60 mL
4. Biotin-conjugated Antibody Solution 100 μL
5. Peroxidase-conjugated Streptavidin Solution 100 μL
6. Luminescent Reagent 1 6 mL
7. Luminescent Reagent 2 6 mL
8. Wash Solution (10 X) 100 mL
9. Sample Buffer 1 0.6mL
10. Sample Buffer 2 30 mL
11. Plate Seal 3 Sheets

4. Performance

Range 4.10-1000 pg/mL
Specifity Total Tau*
Sample** Human cerebrospinal fluid (CSF)
Sample volume 10μL-
Assay time 3 hours
Detection method Luminescence method***

  *Not tested for mouse and rat samples.
  ** Not recommended for plasma and serum.
  *** Requires luminescence plate reader for measurement.

5. Assay Principle

This kit is sandwich ELISA that measures total Tau by using antibodies which recognize the middle region of Tau and C-terminal region.

Tau isoforms

■Phosphorylation sites of Tau

6. Spike-recovery and linearity of dilution

Human CSF 1 Not spiked + 20 pg/mL Tau + 60 pg/mL Tau + 150 pg/mL Tau
Measured Measured Recovery Measured Recovery Measured Recovery
4.09 pg/mL 24.8 pg/mL 104% 58.1 pg/mL 90% 175 pg/mL 114%
Human CSF 2 Not spiked + 40 pg/mL Tau + 135 pg/mL Tau + 380 pg/mL Tau
Measured Measured Recovery Measured Recovery Measured Recovery
24.8 pg/mL 70.5 pg/mL 114% 160 pg/mL 100% 390 pg/mL 96.1%

7. Human CSF measurements at different dementia stages

Cerebrospinal fluids(CSF) from patients were measured with our ELISA kit and competitor’s kit. The samples are from Alzheimer disease (AD) , mild cognitive impairment(MCI) and non-dementia patients.

Wako Company A
A significant difference observed between non-dementia, MCI and Alzheimer's disease.

8. Correlation between Tau concentration and MMSE score

Wako Company A
MMSE score:diagnosis Inquiry test score of cognitive function
30-28 Normal  
27-24 Suspected of mild cognitive impairment
≦23 Suspected of dementia
The high correlation was observed between Tau concentration and MMSE score.

9. Product List

Wako Cat. No Product Name Pkg. Size Grade Strage Condition
296-80401 Tau ELISA Kit Wako 96 tests For Immunochemistry keep at 2-10°C

10. Related Product Lists

Tau Antibodies
Reactive Tau Wako Cat. Code Product Name Pkg. Size Grade Strage Condition
Total Tau 011-26891 Anti Human/Mouse/Rat Tau,
Rat Monoclonal Antibody (RTM38)
10 µL For Immunochemistry Keep at -20°C
017-26893 50 µL Keep at -20°C
Total Tau
(Human Specific)
019-26951 Anti Human Tau,
Rat Monoclonal Antibody (RTM49)
10 µL For Immunochemistry Keep at -20°C
015-26953 50 µL Keep at -20°C
Total Tau
(Mouse specific)
016-26961 Anti Mouse Tau,
Rat Monoclonal Antibody (RTM47)
10 µL For Immunochemistry Keep at -20°C
012-26963 50 µL Keep at -20°C
Phosho-
Tau T181
012-26603 Anti Phosphorylated Tau T181,
Rat Monoclonal Antibody (2E2-A6)
10 µL For Immunochemistry Keep at -20°C
016-26601 50 µL Keep at -20°C
Phosho-
Tau S199
019-26613 Anti Phosphorylated Tau S199,
Rat Monoclonal Antibody (5B8-1E2)
10 µL For Immunochemistry Keep at -20°C
013-26611 50 µL Keep at -20°C
Phosho-
Tau S262
014-27121 Anti Phosphorylated Tau S262,
Rat Monoclonal Antibody (TIP1-35)
10 µL For Immunochemistry Keep at -20°C
010-27123 50 µL Keep at -20°C
Phosho-
Tau S422
016-27681 Anti Phosphorylated Tau S422,
Monoclonal Antibody (AP422)
10 µL For Immunochemistry Keep at -20°C
012-27683 50 µL Keep at -20°C
2N-Tau017-27351 Anti 2N-Tau,
Rat Monoclonal Antibody (2C2)
10 µL For Immunochemistry Keep at -20°C
013-27353 50 µL Keep at -20°C
3R-Tau 012-26583 Anti 3R-Tau,
Rat Monoclonal Antibody (2A1-1F4)
10 µL For Immunochemistry Keep at -20°C
016-26581 50 µL Keep at -20°C
4R-Tau019-26593 Anti 4R-Tau,
Monoclonal Antibody (3E8-1A6)
10 µL For Immunochemistry Keep at -20°C
013-26591 50 µL Keep at -20°C
Tau Proteins
Product Name Wako Cat No. Pkg. Size Grade Storage Condition
Tau-441 Protein, Human, recombinant 205-20331 100 µg For Cell biology keep at - 20°C
Tau-412 Protein, Human, recombinant 208-20321 100 µg For Cell biology keep at - 20°C
Tau-410 Protein, Human, recombinant 201-20311 100 µg For Cell biology keep at - 20°C
Tau-383 Protein, Human, recombinant 204-20301 100 µg For Cell biology keep at - 20°C
Tau-381 Protein, Human, recombinant 201-20291 100 µg For Cell biology keep at - 20°C
Tau-352 Protein, Human, recombinant 204-20281 100 µg For Cell biology keep at - 20°C
Tau Protein 4-Repeat Domain, Human, recombinant 209-20351 100 µg For Cell biology keep at - 20°C
Tau Protein 3-Repeat Domain, Human, recombinant 202-20341 100 µg For Cell biology keep at - 20°C
Aβ oligomer ELISA kit
Product Name Wako Cat. No Pkg. Size Grade Storage Condition
High Molecular Amyloid β Oligomer ELISA Kit Wako 298-80101 96 tests For Immunochemistry keep at 2-10°C

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