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Amyloid β-protein immunohistostain kit
for discriminative staining of Aβ 40 and Aβ 42 deposited in the brain with Alzheimer's disease

· Examples of staining of continuous sections prepared from the brain with Alzheimer's disease
· Features
· Content of the kit
· Staining method
· Examples of staining (staining of continuous sections prepared from the brain with Alzheimer's disease)
· Precautions concerning use or handling
· References
· Storage
· Reagents required other than the kit
· Reagents for deparaffinization, washing and mounting
· Related Products

Package Insert (556 KB/ 8 p)


Examples of staining of continuous sections prepared from the brain with Alzheimer's disease

Photographs supplied by Prof. Takeshi Iwatsubo, Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo.


Features
1. The product stains amyloid β-protein (1-40) and
amyloid β-protein (1-42) specifically by the C-terminal specific antibodies.
2. The staining is highly sensitive due to high antibody specificity.


Kit Contents
1.Anti-amyloid β-protein (1-40) monoclonal antibody (Clone No.BA27) 7 mL x 1
2.Anti-amyloid β-protein (1-42) monoclonal antibody (Clone No.BC05) 7 mL x 1
3.Blocking serum 10 mL x 1
4.Goat Anti-mouse IgG (H+L), Biotin conjugated, 10 mL x 1
5.ABC solution (streptavidin-biotin-peroxidase complex solution) 10 mL x 1
6.Formic acid (90%) 15 mL x 1
7.Trypsin, crystals 50 mg x 1
Note: This kit does not include DAB, please prepare it, separately.


Staining method


Examples of staining (staining of continuous sections prepared from the brain with Alzheimer's disease)


Precautions concerning use or handling
1. Examine optimal conditions including reaction temperature and time depending on tissues.
2. It is difficult to stain Aβ 40 and Aβ 42 on the same section. Please use continuous sections.
3. Change lemosol*, xylene and ethanol for deparaffination after processing about 30 to 40 slides.
     If they are used without changing, the background is intensified.
4. Take care not to dry the sections during staining. Drying weakens staining.
5. Do not use hot paraffin at 60°C or higher for tissue embedding.
6. Handle DAB with care as it is suspected for carcinogenicity.
     Do not use a solution containing a peroxidase inhibitor (NaN3, etc.) for dissolution of DAB and washing.
* Lemosol is a safe solvent alternative to xylene.


References
1. Iwatsubo, T., Odaka, A., Suzuki, N., Mizusawa, H., Nukina, N. and Ihara, Y.: Neuron, 13, 45 (1994)
2. Saido, TC., Iwatsubo, T., Mann, DMA., Shimada, H., Ihara, Y. and Kawashima, S.: Neuron, 14, 457 (1995)
3. Odaka, A., Ishibashi, Y., Kikuchi, T., Kitada, C. and Suzuki, N.: Biochemistry, 34, 10272 (1995)
4. Fukumoto, H., Odaka, A., Suzuki, N., Shimada, H., Ihara,Y.and Iwatsubo, T.: A.J.Pathology, 148, 1 (1996)
5. Fukumoto, H., Odaka, A., Suzuki, N. and Iwatsubo, T.: Neurodegeneration, 5,13 (1996)


Product
Wako Catalog No. Product Name Grade Pkg. Size
299-56701 Amyloid β-Protein Immunohistostain Kit for immunohistological staining for 50 tests
Storage: To be stored at 2 - 10°C, protected from light




Reagents required other than the kit
Wako Cat. No. Product Name Grade Pkg. Size
040-27001 DAB Tablet
(DAB·4HCl 5 mg/tablet)
for Biochemistry 50 tablets
046-27003 100 tablets
049-22831 DAB Tablet (DAB·4HCl 10 mg/tablet) for Biochemistry 50 tablets
045-22833 100 tablets
086-07445 Hydrogen Peroxide
(abt. 30 %)
for probe assay (S.S.G.) 500 mL
081-04215 Special reagent grade 500 mL
080-01186 Wako 1st grade 500 mL
131-09665 Mayer's Hematoxylin Solution
for Pathology Research 500 mL
134-13065 Mayer's Hematoxylin Solution
(x2)
for Pathology Research 500 mL
299-20551 Immunopen a felt-tipped pen type reagent to minimize waste of antibody solution by drawing a circle around the section on the slide glass. 1 piece
295-20553 5 pieces


Reagents for deparaffinization, washing and mounting
Wako Cat. No. Product Name Grade Packaging
122-03991 Lemosol
* An aromatic solvent used as xylene substitutes, derived from citrus
for Pathology Research 1 L
128-03993 3 L
120-04411 Lemosol A
* An aromatic solvent used as xylene substitutes, derived from bark of eucalyptus and pine.
for Pathology Research 1 L
126-04413 3 L
164-18541 0.01mol/L Phosphate Buffered Saline
for washing tissues 1L
162-18547 20 L
208-14691 0.05 mol/L Tris-HCl Buffer Solution (pH7.6) for Pathology Research 1 L
207-15141 Tris-HCl Buffer Powder (0.05 mol/L, pH 7.6) for Pathology Research 1 L x 20
077-04711 0.1 mol/L Glycine-HCl Buffer Solution (pH 2.2) for Pathology Research 1 L
199-11311 Softmount
for Pathology Research 250 mL



Related Products
Wako Catalog No. Product Name Grade Packaging
019-18761 Amyloid β-Protein (1-40)(Trifluoroacetate) for Biochemistry 1 mg
016-18771 Amyloid β-Protein (1-42)(Trifluoroacetate) for Biochemistry 0.5 mg
014-18951 Amyloid β-Protein (1-40)(Hydrochloride) for Biochemistry 1 mg
011-18961 Amyloid β-Protein (1-42)(Hydrochloride) for Biochemistry 1 mg
Products listed in this page are for research use only.  Do not administer each to human.

 

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