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for Glycobiology & Influenza Research
Anti Sia α2-3, MAb (HYB4)

Wako Catalog No. 011-25171 (The catalog number has been changed in September, 2013) (200 μL)

The HYB4 monoclonal antibody is used for detection of sugar chains included in Sia α2-3 Gal motif such as GM3, GM4, IV3 NeuAcnLc4Cer, etc.
It is applicable to a research of the interrelation of influenza viruses with sialoglycoconjugate receptors.


INDEX

    1. Features
    2. Immunostaining
    3. Detection of α2-3 linked glycolipid    
    4. HYB4 MAb reactivity
    5. Product Information      

  PDF Leaflet (420 KB/2p)

  PDF Package Insert (81 KB/2p)

1. Features



  • Detection of Sialyl α2-3 linked sugar chain

  • for research of Influenza virus and Glycobiology

  • for many application for immunological assays (IgG3)


2. HYB4 MAb Immunostaining


HYB4 / DAPIIgG3 / DAPI
  
<Negative Control>
Immunostaining of A549 cells
Primary antibody: HYB4
Secondary antibody: FITC- mouse IgG
DAPI: a DNA marker

(Data: Dr. Kazuya IPJ Hidari of Junior Collage of Aizu,
        Maho Yamaguchi and Dr. Takashi Suzuki of Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka.)



3. Detection of α2-3 linked glycolipid


  1. Glycolipid
    IV3 Neu Ac nLc4 Cer++
    IV6 Neu Ac nLc4 Cer-
    GM4+
    GM3(NeuAc)+++
    GM3(NeuGc)-
    GM2-
    GM1a-
    GD1a-
    GD3-
    nLc4Cer-

  2. Glycoprotein
    N-linked++
    O-linked+



HYB4 epitope:
Sugar chain motif: Neu Ac α 2-3 Gal β1-4Glc (Glc NAc) β1-R














(Data: Dr. Kazuya IPJ Hidari of Junior Collage of Aizu,
          Maho Yamaguchi and Dr. Takashi Suzuki of Department of Biochemistry, School of
          Pharmaceutical Sciences, University of Shizuoka.)

4. HYB4 MAb Reactivity
AB
   
Detection of α2-3 linked glycolipids (TLC-Western blot)


Plate A: Orcinol staining
Plate B: HYB4 Immunostaining

Lane 1: IV3 Neu Ac nLc4 Cer
Lane 2: IV6 Neu Ac nLc4 Cer

Developing solvent:
CHCl3 / CH3OH / 12 mM MgCl2 (50 : 40 : 10 by volume)

(Data: Dr. Kazuya IPJ Hidari of Junior Collage of Aizu,
          Maho Yamaguchi and Dr. Takashi Suzuki of Department of Biochemistry, School of
          Pharmaceutical Sciences, University of Shizuoka.)


 

5. Product Information
  
[Concentration]     Indicated on each label.

[Formulation]  1x PBS (pH 7.2) aqueous solution with 50 % glycerol.

[Clone No.]     HYB4

[Cross Reactivity] GM3, GM4, α2-3 nLc4 Cer

[Subclass]   IgG3·κ

[Working Dilution*]
  SDS-PAGE / Western Blot   10  -  50 μg/mL
  ELISA   5  -  25 μg/mL
  Flow Cytometry:   5  -  25 μg/mL
  Immunocytochemistry:  10 - 100 μg/mL
*: Please optimize the most appropriate concentration for your analysis conditions and samples.

[Storage]   Keep at -20°C. Avoid repeated freezing and thawing.

Product Name Wako Catalog No. (Package Size) Grade
Anti Sia α2-3, Monoclonal Antibody (HYB4) 011-25171   (200 μL) for Immunochemistry
<NOTE> The previous Wako Catalog Number 013-23791 was discontinued.


Products listed in this page are for research use only.  Do not administer each to human.


 

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