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Research for Apoptosis, Inflammation and Degenerative Neurological Disorder
Anti Phosphorylated ASK1, MAb/ Anti ASK1, MAb

  • htm leaflet
  • Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including growth factors and environmental stresses. A MAP kinase kinase kinase (MAPKKK), termed ASK1, was identified that activated two different subgroups of MAP kinase kinases (MAPKK), SEK1 (or MKK4) and MKK3/MAPKK6 (orMKK6), which in turn activated stress-activated protein kinase (SAPK, also known as JNK; c-Jun amino-terminal kinase) and p38 subgroup of MAP kinases, respectively. Overexpression of ASK1 induced apoptotic cell death, and ASK1 was activated in cells treated with tumor necrosis factor-alpha (TNF-alpha). Moreover, TNF-alpha-induced apoptosis was inhibited by a catalytically inactive form of ASK1. ASK1 may be a key element in the mechanism of stress- and cytokine-induced apoptosis.

    Anti Phosphorylated ASK1, Monoclonal Antibody (Wako Catalog No. 017-22351)

    • Reactive with human ASK1 including phosphorylated mouse Thr838 and human Thr845.
    • No cross-reactive with human ASK1 Thr838 Ala mutant.
    • Clone No.: PA214
    • Working Dilution: Western blot 1 : 1,000
  • Package Insert (58kb/1p)
  • Specificity of Anti Phosphorylated ASK1 (PA214)

    ASK1 tagged with Flag was transfected into HEK293A cells and Western blot analhysis was performed using anti-phosphorylated ASK1 antibody (PA214). As a result, the band could be detected only in the Stim lane containing ASK1 overexpressing cells stimulated with H2O2 for phosphorylation.

    (Data were provided by Dr. Maruyama, J. and Noguchi, T., Cell Signaling, Graduate School of Pharmaceutical Sciences, The University of Tokyo)

    ASK‚Phosphorylated

     

    (-) lane control vector
    TA lane Human ASK1, mutated to Ala at Thr838, which is the recognition site for phosphorylation antibody, was overexpressed.
    WT lane Wild type human ASK1 was overexpressed.
    Stim lane Wild type human ASK1 was overexpressed and H2O2 stimulation was conducted for activation.

    Anti ASK1, Monoclonal Antibody (Wako Catalog No. 010-22341)

    • Reactive with human and mouse ASK1.
    • No cross-reactive with ASK2.
    • Clone No.: TC003
  • Package Insert (54kb/1p)
  • Specificity of Anti ASK1 (TC003)

    Bone marrow-derived macrophages were extracted from ASK1 (+/+) and ASK1 (-/-) mice and Western blot analysis was performed using ASK1 antibody (TC003). As a result, samples derived only from ASK1 (+/+) mice could be detected.

    (Data were provided by Dr. Maruyama, J. and Noguchi, T., Cell Signaling, Graduate School of Pharmaceutical Sciences, The University of Tokyo)

    ASK

     

    References:

    1. Ichijo, H., Nishida, E., Irie, K., et al.: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.", SCIENCE, 275, 90-94 (1997)
    2. Tobiume, K., Saitoh, M., Ichijo, H., et. al.: "Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of pre-formed oligomer: J. Cell Physiol., 191, 95-104 (2002)

    Product List

    Description Wako Catalog No. Package Size Storage Condition
    Anti Phosphorylated ASK1, Monoclonal Antibody (Clone No. PA214)
    017-22351
    50 μg
    -20 degrees C
    Anti ASK1, Monoclonal Antibody (Clone No. TC003)
    010-22341
    50 μg
    -20 degrees C
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