Autophagy Research
Anti SQSTM1/A170/p62,
Rabbit
(Wako Catalog No. 018-22141; 100 µL)
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Sequestosome 1 (SQSTM1)/A170 (mouse)/p62 (human)/ZIP (rat) a ubiquitin-binding
protein, expresses oxidative stress-dependently. Abnormality of SQSTM1 leads to bone
metabolic disorder, obesity and Type Ⅱ diabetes. SQSTM1 was reported to bind LC3,
which regulates autophagosome formation. The protein has attracted the attention of
researchers because it is believed to induce a protein from ubiquitin-proteosome system
(UPS) to the lysosome-dependent macroautophagy (autophagy) system, which are two
major intracellular pathways for protein degradation.
Wako has launched the mouse SQSTM1 (A170) rabbit antiserum, which is applicable to
Western blot, immunohistochemistry and immunofluorescence.
Preparation
Immunogen
Specificity
Working Dilution
The antiserum is diluted with an equal amount of PBS and absorbed with
E. coli proteins.
recombinant murine SQSTM1 (A170)(AA254-333) containing T7 tag at the
N-terminal end and His tag at the carboxy-terminal end
Specific for mouse and rat SQSTM1 (A170/ZIP).
Slightly reactive with human SQSTM1 (p62).
Western blot
Immunohistochemistry
Immunofluorescence
1 : 200
1 : 1,000
1 : 1,000
Application
Immunohistochemistry
Figure: Brain tissues were fixed with 4 % paraformaldehyde and embedded with paraffin.
The 6 µm sections were stained with avidin-biotin-peroxidase method.
Primary Antibody : Anti SQSTM1/A170/p62(Wako Cat. #018-22141) 1 : 1,000
Secondary Antibody : Anti rabbit IgG, biotin conjugated
Data was provided by Dr. Nakaso, Tottori University (Japan)
Western blot
Figure:
Western blot of cultivated mouse vascular
smooth-muscle cell lysate (20µg)
Antibody:
Anti SQSTM1/A170/p62(Wako
Cat. #018-22141) 1 : 200
Data was provided by Dr. Ishii, Tsukuba University (Japan)
Reference
1）
Ishii, T., Yanagawa, T., Kawane, T., Yuki, K., Seita, J., Yoshida, H. and Bannai, S.: “Transcription factor Nrf2
coordinately regulates a group of oxidative stress-inducible genes in macrophages”, Biochem. Biophys. Res.
Commun., 226, 456-60 (1996).
2）
Ishii, T., Itoh, K., Takahashi, S., Sato, H., Yanagawa, T., Katoh, Y., Bannai, S. and Yamamoto, M.: “Transcription
factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages”, J. Biol. Chem.,
275, 16023-9 (2000).
3）
Komatsu, M., Waguri, S., Koike, M., Sou, Y.S., Ueno, T., Hara, T., Mizushima, N., Iwata, J., Ezaki, J., Murata, S.,
Hamazaki, J., Nishito, Y., Iemura, S., Natsume, T., Yanagawa, T., Uwayama, J., Warabi, E., Yoshida, H., Ishii, T.,
Kobayashi, A., Yamamoto, M., Yue, Z., Uchiyama, Y., Kominami, E. and Tanaka, K.: “Homeostatic levels of p62
control cytoplasmic inclusion body formation in autophagy-deficient mice”, Cell, 131, 1149-63 (2007).
4）
Nakaso, K., Kitayama, M., Ishii, T., Bannai, S., Yanagawa, T., Kimura, K., Nakashima, K., Ohama, E. and
Yamada, K. : “Effects of kainate-mediated excitotoxicity on the expression of rat counterparts of A170 and
MSP23 stress proteins in the brain”, Brain Res. Mol. Brain Res., 69, 155-63 (1999).
Description
Wako Catalog No.
Package Size
Anti SQSTM1/A170/p62, Rabbit
for Immunochemistry, Keep at -20ºC
018-22141
100µL
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