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High-sensitivity apoptotic DNA ladder detection kit using fluorescence
Apoptotic Ladder Detection Kit Wako

In apoptotic cells, endonuclease activation causes cleavage of chromatin DNA into short internucleosomal fragments of roughly 180 base pairs and multiples thereof. This kit can quickly detect DNA ladder in apoptotic cells in a reproducible manner. It uses SYBR® Green I which is about 25-times more sensitive than ethidium bromide.


 INDEX

  1. Features
  2. Kit Components
  3. Operating Procedure
  4. Plinciple
  5. Data
  6. Product List
  7. Related Products
  8. Links to Manual PDF file (1.6 MB / 18 pages)



1. Features
  • Highly sensitive: Can detect 103 cells
  • The entire procedure can be completed in about 2 hours and 30 minutes.
  • Safe; no phenol or chloroform used
  • The original DNA extraction method obtains a clear ladder.


2. Kit Components
  for 24 lanes for 96 lanes
1. Enzyme Reaction Solution 4.5mL 18 mL
2. RNase 250 μL 1 mL
3. Enzyme Activator 500 μL 2 mL
4. Protein Digestion Enzyme 250 μL 1 mL
5. DNA Extraction Solution 7.5 mL 30 mL
6. TE Buffer 375 μL 1.5 mL
7. Loading Buffer 50 μL 200 μL
8. Ladder Marker(123 bp) 50 μL 200 μL
9. SYBR® Green I * 25 μL 100 μL
* "SYBR®" is a registered trademark of Life Technologies.


3. Operating Procedure
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4. Principle



5. Data

Percentage of apoptotic cells in 105 cells (%)


Apoptosis of HL-60 cells was induced with actinomycin D, and the ratio between apoptotic cells and non-apoptotic cells was adjusted as shown to the right. DNA was then extracted from the HL-60 cells, and the DNA ladder viewed with this kit.
In 100% (105 apoptotic cells) to 1% (103 apoptotic cells) samples, nucleosome-sized DNA fragmentation was detected.



6. Product List
Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
Apoptosis Ladder Detection Kit Wako for Apoptosis Research for 24 Lanes
for 96 Lanes
297-71401
293-71403
Keep at 2-10°C


7. Related products
Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
Agarose S Nippon Gene Co., Ltd. 5g
100g
316-01191
312-01193
Keep at
room temperature
Apoptosis in situ Detection Kit Wako for Apoptosis Research 40 tests 293-71501 Keep at -20°C



8. Link
Links to Manual PDF file (1.6 MB / 18 pages)
QUOTATION REQUEST for reagents for Apoptosis Research

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Note
  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
  • Please visit our online catalog to search for other products from Wako: http://www.e-reagent.com
  • This information may contain products that cannot be exported to your country due to regulations.
  • Bulk quote requests for some products are welcomed. Please contact us or our distributor near you.
  • shows the storage condition should be at 2 - 10°C.
Home > Products > Life Science > Apoptotic Ladder Detection Kit Wako