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for animal cell culture
Cell culture-related products

Products such as liquid media, equilibrated salt solutions, trypsin-EDTA solutions, antibiotic solutions and extracellular substrates are fully provided.



INDEX
  1. New products
    · Sterilized Water, Endotoxin-Free
    · Laminin Solution
    · Fibronectin Solution
  2. Liquid Media
  3. Equilibrated Salt Solutions
  4. Trypsin Solutions for Separation of Adhering Cells and Dispersion of Tisse Cells
  5. Antibiotic Solutions
  6. Medium-added Solutions, etc.
  7. L-Alanyl-L-glutamine



1.   New products

  · Sterilized Water, Endotoxin-Free
This is sterilized water by filtration.
- Endotoxin specification: Not more than 0.01 EU/mL
♣ Quality tests: Appearance, endotoxin, sterility

Product Name Package Size Wako Catalog No.
Sterile Water, Endotoxin Free 500 mL 196-15645


  · Laminin Solution
  · Fibronectin Solution

Laminin and fibronectin are extracellular substrates used as coating agents of cell culture containers. The extracellular substrates play important roles in proliferation, differentiation and morphogenesis of cells. In particular, they are used as coating agents of cell culture containers.

Product Name Package Size Wako Catalog No.
Laminin Solution, from Mouse EHS Tumor 1 mg 120-05751
Fibronectin Solution, from Human Plasma 1 mg 063-05591

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2.   Liquid media
These are general-purpose liquid media such as D-MEM, E-MEM and RPMI-1640. They have been sterilized by filtration and are used as they are by warming to the culture temperature (about 37°C).
♣ Quality tests: Tests for appearance, osmotic pressure, pH, endotoxins, mycoplasma, cell culture and sterility

Product Name L-glutamine Phenol red Sodium pyruvate HEPES Package Size Wako Catalog No.
D-MEM High Glucose Ο Ο - - 500 mL 044-29765
Ο Ο Ο - 500 mL 043-30085
Ο Ο - Ο 500 mL 048-30275
- Ο - - 500 mL 045-30285
- Ο Ο - 500 mL 045-32245
- Ο - Ο 500 mL 042-32015
- - - - 500 mL 040-30095
Low Glucose Ο Ο Ο - 500 mL 041-29775
No Glucose Ο Ο - - 500 mL 042-32255
E-MEM Ο Ο - - 500 mL 051-07615
- Ο - - 500 mL 056-08385 *1
G-MEM Ο Ο - - 500 mL 078-05525
MEMα Ο Ο Ο - 500 mL 135-15175
Ο Ο Ο - 500 mL 137-17215 *2
Ο - Ο - 500 mL 134-17225 *2
RPMI-1640 Ο Ο - - 500 mL
1 L
189-02025
187-02021
Ο Ο - Ο 500 mL 189-02145
Ο Ο Ο Ο 500 mL 187-02705 *3
Ο - - - 500 mL 186-02155
- Ο - - 500 mL 183-02165
Ham's F-12 Ο Ο Ο - 500 mL 087-08335
Ham's F-12K
(Kaighn's Modification)
Ο Ο Ο - 500 mL 080-08565
D-MEM/Ham's F-12 Ο Ο Ο - 500 mL 048-29785
- Ο Ο - 500 mL 046-32275 *4
Ο Ο Ο Ο 500 mL 042-30555
Ο - Ο - 500 mL 045-30665
Ο - Ο Ο 500 mL 049-32265
- Ο Ο Ο 500 mL 042-30795
IMDM Ο Ο Ο Ο 500 mL 098-06465

*1: containing non-essential amino acids
*2: containing nucleoside
*3: containing 4,500 mg/L Glucose
*4: containing L-Alanyl-L-glutamine
NOTE: Each Wako Catalog No. links to each composition list


3.   Equilibrated Salt Solutions
D-PBS(-), PBS(-), HBSS(-) and HBSS(+) are lined up. They are used for washing and dilution of cells.
♣ Quality tests: Tests for appearance, osmotic pressure, pH, endotoxins, mycoplasma and sterility

Product Name Package Size Wako Catalog No.
D-PBS(-) *5, 6 500 mL 045-29795
10x D-PBS(-) *5, 6 500 mL 048-29805
PBS(-) *5, 6 500 mL 166-23555
10x PBS(-) *5, 6 500 mL 163-25265
HBSS(-) with Phenol Red *6 500 mL 084-08345
HBSS(-) without Phenol Red *6 500 mL 085-09355
HBSS(+) with Phenol Red *6 500 mL 082-09365
HBSS(+) without Phenol Red *6 500 mL 084-08965
*5: D-PBS(-) and 10x D-PBS(-) contain KCl due to formulation of Dulbecco but PBS (-) does not contain KCl.
*6: (+) contains Mg2+ and Ca2+ but (-) does not contain these ions.
NOTE: Each Wako Catalog No. links to each composition list




4.   Trypsin Solution for Separation of Adhering Cells and Dispersion of Tissue Cells
These solutions may be used for separation of adhering cells and dispersion of various tissue cells.
♣ Quality tests: Tests for appearance, osmotic pressure, pH, endotoxins, mycoplasma, sterility, practical test and virus test*7.
*7: Trypsin (1:250) tested for porcine parvovirus is used.

Product Name Package Size Wako Catalog No.
0.05 w/v % Trypsin - 0.53 mmol/L EDTA·4Na Solution with Phenol Red 100 mL
500 mL
202-16931
204-16935
0.25 w/v % Trypsin - 1 mmol/L EDTA·4Na Solution with Phenol Red 100 mL
500 mL
209-16941
201-16945
0.5 w/v% Trypsin - 5.3 mmol/L EDTA·4Na Solution without Phenol Red (x10) 100 mL 208-17251
0.5 w/v% Trypsin - 5.3 mmol/L EDTA·4Na Solution with Phenol Red (x10) 100 mL 206-17291

NOTE: Each Wako Catalog No. links to each composition list

Related Products
 Collagenase from Clostridium histolyticum

Product Name Package Size Wako Catalog No.
Collagenase for Cell Dispersion (crude type) 100 mg
1 g
5 g
038-22361
034-22363
032-22364
Collagenase Type I for Cell Dispersion 100 mg
500 mg
1 g
031-17601
037-17603
035-17604
Collagenase Type V for Cell Dispersion 100 mg
1g
038-17851
032-17854
Collagenase Type X for Cell Dispersion 100 mg
500 mg
1 g
035-17861
031-17863
039-17864




5.   Antibiotic solutions

These are solution types of antibiotics used for prevention of various microorganisms during cell culture. They are sterilized by filtration and can be used as they are by adding a necessary amount to a liquid medium.
♣ Quality tests: Tests for appearance, osmotic pressure, pH, endotoxins, mycoplasma and sterility

Product Name Target of Activity Pkg. Size Wako Cat. No.
Gram-positive
bacteria
Gram-negative
bacteria
Yeast Fungus Mycoplasma
Amphotericin B Suspension     Ο Ο   50 mL 019-23891
Gentamicin Sulfate Solution *8 Ο Ο     Ο 10 mL 078-06061
Kanamycin Sulfate Solution *9 Ο Ο     Ο 20 mL 117-00961
1 mg/mL Mitomycin Solution Ο Ο       1 mL 133-15931
Penicillin-Streptomycin Solution (x50) Ο Ο       100 mL 164-25251
Penicillin-Streptomycin Solution (x100) Ο Ο       100 mL 168-23191
Penicillin-Streptomycin-Amphotericin B Suspension (x100) Ο Ο Ο Ο   100 mL 161-23181
Penicillin-Streptomycin-L-Glutamine Solution (x100) Ο Ο       100 mL 161-23201

*8: 50 mg/mL Gentamicin Sulfate Solution in water.
*9: 50 mg/mL Kanamycin Sulfate Solution in 0.85 w/v% Sodium Chloride Solution
NOTE: Each Wako Catalog No. links to each composition list




6.   Solutions to be added to Media

Concentrated solutions of medium constituents and 30 w/v% albumin solution (derived from cow serum) are available. These can be used for addition to media not containing various ingredients and for increasing concentrations of various ingredients in the medium. They are sterilized by filtration and can be used as they are by adding a necessary amount to a liquid medium.
♣ Quality tests: Tests for appearance, osmotic pressure, pH, endotoxins, mycoplasma and sterility

Product Name Package Size Wako Catalog No.
30 w/v% Albumin Solution, from Bovine Serum, Fatty Acid Free
50 mL 017-22231
30 w/v% Albumin D-PBS(-) Solution, from Bovine Serum, Fatty Acid Free
50 mL 015-23871
7.5 w/v% Albumin D-PBS(-)Solution, from Bovine Serum
100 mL 012-23881
200 mmol/L L-Glutamine Solution (x100) 100 mL 073-05391
45 w/v% D(+)-Glucose Solution 500 mL 079-05511
Insulin Solution, Human, recombinant *10 100 mL 093-06351
MEM Essential Amino Acids Solution (x50) 100 mL 132-15641
MEM Non-essential Amino Acids Solution (x100) 100 mL 139-15651
MEM Vitamin Solution (x100) 100 mL 130-17141
7.5 w/v% Sodium Bicarbonate Solution 100 mL 195-16411
100mmol/L Sodium Pyruvate Solution (x100) 100 mL 190-14881
Sterile Water, Endotoxin Free *11 500 mL 196-15645
*10: 10 mg/mL Insulin Solution in water
*11: Endotoxin: max. 0.01 EU/mL
NOTE: Each Wako Catalog No. links to each composition list





7.   L-Alanyl-L-glutamine

This is a peptide containing L-glutamine. L-glutamine may affect cultured cells adversely as it is degraded spontaneously in a solution and produces ammonia. L-alanyl-glutamine is unlikely to degrade spontaneously even in a solution and can be used alternatively to L-glutamine. When added in a medium, it improves cell proliferation and allows a long-term culture. Our company offers a solution and a powder.

Product Name Package Size Wako Catalog No.
200 mmol/L L-Alanyl-L-Glutamine Solution (x100) 100 mL 016-21841
L-Alanyl-L-Glutamine 5 g 010-22581
25 g 018-22582
100 g 016-22583

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Revised in July, 2012
Note
  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
  • Please visit our online catalog to search for other products from Wako: http://www.e-reagent.com
  • This information may contain products that cannot be exported to your country due to regulations.
  • Bulk quote requests for some products are welcomed. Please contact us or our distributor near you.



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