Recombinant peptide based on human collagen type I
Fujifilm developed a non-animal based recombinant peptide (RCP), taking advantage of the know-how from its photographic film past. RCP is a biomaterial, applicable in various forms such as spones, granules and film for regeneration madicine research.
- cellnest Coating Standard Protocol
- cellnest Coating Protocol for various cell lines · 3T3L1 · BHK-21 · C2C12 · CHO-K1 · CV-1 · hMSC
- Protocol to make a cellnest sponge
· HUVEC · MC3T3E1 · MDCK · NHEK · Vero
■ No animal-derived ingredients (xeno-free product) ···
cellnest is manufacturing with recombinant technology from yeast with high reproducible quality
■ High cellular adhesiveness ··· Arg-Gly-Asp (RGD) enriched with high cellular adhesion
■ Biodegradable and bioabsorbable ···
Best for medical research material since readily biodegradable and bioabsorbable material which will not remain within the body.
■ Uniform molecular weight ··· For formulation into various forms, such as sponges, porous particles and granules
■ Low endotoxin (max. 10 EU/g)
●○● Material design ●○●
●○●Highly Flexible Formulation●○●
The peptide has an extremely uniform molecular weight distribution of approximately 51kD and is easy to formulate into various forms such as sponges, porous particles and granules.
●○● Stable manufacturing quality ●○●
Due to a recombinant peptide manufactured from yeast, the reliable supply with high reproducible quality is guaranteed.
A test with HUVEC on a petri dish coated with cellnest recombinant peptide based on human collagen type I has proven to enhance the adhesion of cells in a good form with good extensibility.
●○● Actual cells tested ●○●
endothelial cells (HUVEC), keratinocytes (NHEK), myoblasts (C2C12), pheochromocytoma (PC12), fibroblasts (3T3-L1), osteoblast-like (MC3T3E1), epithelial cell-like（CHO-K1), epitheloal cells(Vero, MDCK) and kidney cells(CV-1)
Kentaro Nakamura, Yasuhiko Tabata.
A new fluorescent imaging of renal inflammation with RCP.
Journal of Controlled Release, Volume 148, Issue 3, Pages 351-358
Kentaro Nakamura, Reiko Iwazawa & Yasuhiro Yoshioka.
Introduction to a new cell transplantation platform via recombinant peptide petaloid pieces and its application to islet transplantation with mesenchymal stem cells.
Transplant International, Volume 29, Issue 9, September 2016, Pages 1039-1050
5. Product List
0.1% Solution volume: 20 mL
Lyophilized volume: 100 mg
|Wako Cat. No.||Product Name||Package Size|
cellnest, recombinant peptide based on human collagen type I 0.1% solution
cellnest, recombinant peptide based on human collagen type I Lyophilized
cellnest, recombinant peptide based on human collagen type I Bulk product
cellnest, recombinant peptide based on hyman collagen type I processed goods