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For ES cells and iPS cells research
CultureSure low-molecular compounds

They are low-molecular compounds that have been reported to be involved in the maintenance of undifferentiated potency and induction of differentiation of ES cells and iPS cells.
This product can be used for cell culture safely, because of being conducted mycoplasma test, endotoxin test and confirmation of cell toxicity.


1. CultureSure A-83-01

A-83-01
  • Endotoxin tested, mycoplasma negative tested.
  • Cytotoxicity confirmed ※1
  • ※ 1 Using Human iPS 201B7 strain cells



A-83-01
It is a selective inhibitor of ALK4, ALK5 and ALK7. It inhibits the phosphorylation of Smad2 / 3 and the epithelial-mesenchymatous tissue conversion induced by TGF-β.
This product does not effect the bone morphogenic factor typeⅠreceptor, p38 MAP kinase and the extracellular-regulated kinase hardly or at all.
Further, by adding to the culture medium of rat iPS cells, it has been reported to proliferate the rat iPS cells uniformly without differentiation, and be able to culture for a long period.


CAS No.909910-43-6
C25H19N5S=421.52

Product Outline

  • Assay(HPLC): min.98.0%
  • Appearance: White~yellow, crystalline powder~powder or mass
  • Solubility: Soluble in DMSO
  • Mycoplasma negative tested.
  • Endotoxin: less than 0.01EU / mg
  • Storage conditions: -20°C, stored in a shading state (inert gas filled).
  • Bibliography: Li, W., et al.: Cell Stem Cell, 4, 16(2009).

2. CultureSure CHIR99021

CHIR99021
  • Endotoxin tested, mycoplasma negative tested.
  • Cytotoxicity confirmed※1
  • ※ 1 Using Human iPS 201B7 strain cells



CHIR99021
It is a highly selective inhibitor for GSK-3β. This product does not show cross-reactivity against CDKs.
ES cells cultured in a medium containing CHIR99021,PD0325901 have been reported to be suppressed the differentiation with high efficiency.



CAS No.252917-06-9
C22H18Cl2N8=465.34

Product Outline

  • Assay(HPLC): min.97.0%
  • Appearance: White~pale brown, crystalline powder~powder
  • Solubility: Soluble in DMSO, methanol.
  • Mycoplasma negative tested.
  • Endotoxin: less than 0.05EU / mg
  • Storage conditions: -20°C, stored in a shading state.
  • Bibliography: Ying, QL., et al .: Nature, 453, 519 (2008).

3. CultureSure CKI-7 Dihydrochloride

CKI-7 Dihydrochloride
  • Endotoxin tested, mycoplasma negative tested.
  • Cytotoxicity confirmed ※1
  • ※ 1 Using Human iPS 201B7 strain cells



CKI-7 Dihydrochloride
It is one of the isoquinoline-induced type, and inhibits casein kinase 1 (CK1) selectively in competition with ATP.
CK1 is serine / threonine kinases, and controls the nuclear export, cell cycle, cell division, apoptosis etc,.
By using together with SB431542 and Y-27632, it has been reported to induce differentiating human ES cells and human iPS cells into retinal progenitor cells under serum-free and feeder-free conditions.


CAS No.1177141-67-1
C11H12ClN3O2S・2HCl=358.67

Product Outline

  • Assay(HPLC): min.98.0%
  • Appearance: White~pale brown, crystalline powder~powder
  • Solubility: Soluble in water.
  • Mycoplasma negative tested.
  • Endotoxin: less than 0.05EU / mg
  • Storage conditions: -20°C, stored in a shading state(inert gas filled).
  • Bibliography: Takahashi, M. et al.: J. Cell Sci., 122, 3169 (2009).


4. CultureSure IWP-2

IWP-2
  • Mycoplasma negative tested.
  • Cytotoxicity confirmed ※1
  • ※ 1 Using Human iPS 201B7 strain cells



IWP-2
It inactivates Porcupine (Porcn) of the membrane-bound o-acyltransferase (MBOAT), and suppresses palmitoylation of Wnt protein.
Thereby, it inhibits the phosphorylation of Wnt-dependent Lrp6 receptor and the accumulation of Dvl2 and β-catenin.
It has been reported to promote the differentiation into myocardial cells from human iPS cells.



CAS No.686770-61-6
C22H18N4O2S3=466.60

Product Outline

  • Appearance: White~pale brown, crystal~powder
  • Solubility: Soluble in DMSO
  • Mycoplasma negative tested.
  • Storage conditions: 2~10°C, stored in a shading state(inert gas filled).
  • Bibliography: Minami, I. et al.: Cell Rep., 2, 1448 (2012).

5. CultureSure SB431542

SB431542
  • Endotoxin tested, mycoplasma negative tested.
  • Cytotoxicity confirmed ※1
  • ※ 1 Using Human iPS 201B7 strain cells



SB431542
It is a selective inhibitor of activin receptor-like kinase (ALK) of the TGF-β superfamily type 1, and inhibits ALK4,5,7. It has been reported to proliferate and differentiate ES cell-derived endothelial cells and stimulate the sheet formation.
In addition, using this product together with thiazovivin and PD0325901, it has been reported that the reprogramming efficiency would improve more than 200 times and the reprogramming speed would become faster.


CAS No. 301836-41-9
C22H16N4O3=384.39

Product Outline

  • Assay(HPLC): min.98.0%
  • Appearance: white ~ slightly pale yellowoish brown, crystalline powder - powder
  • Solubility: Soluble in DMSO, acetonitrile, acetone, ethanol.
  • Mycoplasma negative tested.
  • Endotoxin: less than 0.05EU / mg
  • Storage conditions: -20°C, stored in a shading state.
  • Bibliography:
    Ogawa, K., et al.: J. Cell. Sci., 120, 55 (2007).
    Lin, T., et al.: Nat. Methods, 6, 805 (2009).

6. CultureSure Y-27632

Y-27632
  • Endotoxin tested, mycoplasma negative tested.
  • Cytotoxicity confirmed ※1
  • ※ 1 Using Human iPS 201B7 strain cells, human chronic myeloid leukemia K562 strain cells



Y-27632
It is a selective and potent ROCK inhibitor. Y-27632 has various actions such as the contraction of vascular smooth muscle by the signaling system of ROCK, etc,.
It has been reported to suppress the cell death at the time of cell dispersion of human ES cells and human iPS cells, also improve the cell survival rate after cryopreservation.
[ROCK: Rho-associated coiled-coil forming kinase / Rho-linked kinase / serine-threonine protein phosphorylation enzyme]


CAS No.331752-47-7
C14H21N3O・2HCl・H2O=338.27

Product Outline

  • Appearance: white ~ pale yellow, crystalline powder - powder
  • Assay (HPLC): 98.0+ %
  • Solubility: Soluble in water, ethanol
  • Specific optical rotation [α] D20(c=0.5, CH3OH) :+6.3 ° (first lot actual).
  • Mycoplasma negative tested.
  • Endotoxin: less than 0.025 EU/mg
  • Storage conditions: -20°C, stored in a shading state (inert gas filled).

7. References

Ito, H., et al.: Liver Int., 32, 592 (2012).
Kawamata, M., et al.: Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).
Claassen, DA., et al.: Mol. Reprod. Dev., 76, 722(2009).
Martin-Ibanez, R., et al.: Hum. Reprod., 23, 2744(2008).
Watanabe, K., et al.: Nat. Biotechnol., 25, 681(2007).
Sakamoto, K., et al.: J. Pharmacol. Sci., 92, 56(2003).
Nishimaru, K., et al.: J. Pharmacol. Sci., 92, 424(2003).
Uehata, M., et al.: Nature, 389, 990 (1997).


                     

8. Product List

    < for Cell Culture >
Product Name Package Size Wako Catalog No. Storage Condition
CultureSure A-83-01 2 mg 039-24111 Keep at -20°C.
10 mg 035-24113
CultureSure CHIR99021 1 mg 038-23101
5 mg 034-23103
CultureSure CKI-7 Dihydrochloride 5 mg 035-23971
CultureSure IWP-2 5 mg 034-24301 Keep at 2-10°C.
25 mg 030-24303
CultureSure SB431542 5 mg 031-24291 Keep at -20°C.
25 mg 037-24293
CultureSure Y-27632 1 mg 030-24021
5 mg 036-24023
25 mg 034-24024

9. Related Products

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