Nissan Chemical Industries, Ltd.
New three-dimensional culture medium FCeM® series
FCeM® is a three-dimensional culture medium containing a combination of polymer "FP001" which uniformly disperses the cells clumps.
By the effect of the FP001-containing medium, it maintains / promotes the growth and function of cells in a non-adhesive state.
■It enables the suspension culture of spheres (spheroids).
■It suppresses excessive agglomeration of spheres.
It improves proliferative capacity and gets the original function of cells
It improves penetration of drugs and nutritional ingredients
■It allows to create a sphere from a single cell
■Viscosity is equivalent to water, easy to add compound
■Animal-derived materials such as BSA are not included
- 1. Features
- 2. Confirmation of anticancer drug sensitivity
- 3. Non-adherent culture of cancer cells
- 4. Effects of HB-EGF to human ovarian cancer · SKOV3 cell proliferation
- 5. Anchorage-independent growth
- 6. Product List
- 7. Related Products
FCeM® uniformly disperses cell clumps while showing the same viscosity as water.
It makes it possible to culture floating spheres (cell mass) at a low concentration (0.015%).
【The growth of cancer cells (cultured for 5 days)】
FCeM® suppresses the formation of giant clumps by A549 and HCT116 and forms a uniform spheres.
【The nature of the medium containing FP001】
Easy to handle as viscosity is equivalent to water and the conventional medium
【Actually used cells 】
FCeM® series can be used to a variety of cancer cells.
2. Confirmation of anticancer drug sensitivity
【Effects of existing anti-cancer drugs】
Comfirmation of s sensitivity of various anti-cancer drugs by using the FP001-containing medium
【FP001 / 384well low adhesive plate three-dimensional culture】
We also confirmed the sensitivity of anti-cancer drugs in 384well plate, and made sure that this is a test system which can withstand even in HTS assessment.
3. Non-adherent culture of cancer cells
Concerning proliferation of A549 cells and HCT116 cells on low attachment plates Using FCeMR, the number of cells (WST value) in FCeMTM was 10 times higher at the maximum compared to that in the control culture medium.
4. Effects of HB-EGF to human ovarian cancer · SKOV3 cell proliferation
We confirmed the growth of HB-EGF dependence in SKOV3 cells ⇒ which enables to optimize the evaluation system by the addition of growth factors
5. Anchorage-independent growth
【Evaluation of specific anchorage-independent growth by growth factors】
In culture method which combines low attachment plate and FP001, the anchorage-independent growth of human ovarian cancer-derived SKOV3 cells by EGF receptor ligand and TGF-β can be evaluated.
【Inhibitory effect of Gefitinib against the anchorage-independent growth by HB-EGF
In culture method which combines low attachment plate and FP001, against the anchorage-independent growth by HB-EGF,Gefitinib as an EGF receptor inhibitor exhibits specific inhibitory effects.
【Susceptibility test for Hela, A549 of Trametinib】
In the three-dimensional culture using FCeM®-D medium, the sensitivity of the EGF signaling pathway inhibitor increases.
|Two-dimensional culture||FP001 Three-dimensional culture|
6. Product List
|Product Name||Base medium||Package Size||Wako catalog No.||Nissan Chemical's product No.||Storage Condition|
|FCeM®-D||D-MEM||500 mL||387-06265||SJ000546||Keep at 2-10°C.|
7. Related ProductsThey are kits to prepare FCeM® medium with your medium in stock. You can prepare the 50 mL medium using the kit.
|Product Name||Package Size||Wako catalog No.||Storage Condition|
|FCeM™-series Preparation kit||1 test||385-07981||Keep at 2-10°C.|
|FCeM™-series Preparation kit for Stem Cells||1 tests||382-07991|