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For coating of cell culture vessels
Fibronectin solution, from human plasma

Fibronectin is glycoprotein of the extracellular matrix. It consists of a dimer of two polypeptide chains, each about 250 kDa.
Fibronectin promotes adhesion of mainly fibroblasts, hepatocytes, and neurons. In addition to cell adhesion, it is also involved in cell migration, promotion of phagocytosis, and tissue repair mediated by integrins, receptors on cell membrane.

INDEX

  1. Product Overview
  2. Example of Use
  3. Main Cell Types
  4. Procedure
  5. Product List
  6. Other Extracellular Matrices
  7. Links to Cell Culture-related Products



1. Product Overview
  • Concentration: 0.5 mg/mL (value of the first lot)
  • Composition: 20 mmol/L Tris-HCl (pH 7.0) + 450 mmol/L NaCl + 12% glycerol
  • Sterilized by filtering through a 0.2 μm filter
  • Store at -80°C (stable for 1 year at -20°C)


2. Example of use

The fibronectin solutions were dissolved to target concentrations with D-PBS(-). These solutions were pipetted (0.3 mL) each into wells of a 24-well plate and incubated overnight at 37°C. After aspiration of the supernatant and blocking with BSA, TIG-3 cells were seeded at the concentration of 5 x 104/mL and incubated at 37°C for 90 minutes. After washing to remove non-adhering cells, adhering cells were removed with trypsin-EDTA solution and counted.
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3. Main Cell Types
  • Fibroblasts
  • Hepatocytes
  • Neurons


4. Sample procedure
  1. Dissolve slowly at 4°C or on ice.
  2. Dilute with D-PBS(-) or a medium to the target concentration of 5 to 30 μg/mL.
  3. Add an appropriate amount of the diluted fibronectin solution to the culture vessel to be coated.
  4. Incubated for 2 hours at 37°C or overnight at 4°C.
  5. Remove the coating solution.
  6. Wash 2 to 3 times with D-PBS(-).
  7. Seed the cells.


5. Product List
Product Name Grade Pkg. Size Wako Cat. No.
Fibronectin Solution, from Human Plasma for Cell Culture 1 mg 063-05591
QUOTATION REQUEST for Fibronectin Soln. for coating of cell culture vessels
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6. Other extracellular matrices
Product Name Grade Pkg.Size Wako Cat. No.
Laminin Solution, from Mouse EHS Tumor for Cell Culture 1 mg 120-05751
Collagen, Type I, from Salmon Skin for Biochemistry 200 mg 031-19443
Elastin, Water Soluble, from Bovine Neck Ligament for Cellbiology 100 mg 054-07421
Elastin, Water Soluble, from Horse Neck Ligament for Cellbiology 100 mg 053-07491
Elastin, Water Soluble, from Porcine Aorta for Cellbiology 100 mg 056-07481
Elastin-A, Water Soluble, from Porcin Aorta for Cellbiology 100 mg 050-07761
Elastin-B, Water Soluble, from Porcin Aorta for Cellbiology 100 mg 057-07771
Elastin-C, Water Soluble, from Porcine Aorta for Cellbiology 100 mg 054-07781
Elastin-D, Water Soluble, from Porcine Aorta for Cellbiology 100 mg 051-07791


7. Link
Links to Cell Culture-related Products
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Note

  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
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