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The weighing and pH adjustment are unnecessary.
The prepared buffer solution, buffer powder

They are phosphate buffer solution and tris buffer solution widely used in biochemical experiments.



1. Phosphate buffer solutions

1/15mol/L Phosphate Buffer Solutions, Autoclaved

This is 1/15mol/L Phosphate Buffer Solutions prepared with sodium dihydrogenphosphate (NaH2PO4) and disodium hydrogenphosphate(Na2HPO4).
Autoclaved.

Product Name Package Size Wako Catalog No. Grade
1/15mol/L Phosphate Buffer Solution, pH6.0 500 mL 166-27195 for Biochemistry
1/15mol/L Phosphate Buffer Solution, pH6.4 500 mL 169-27205
1/15mol/L Phosphate Buffer Solution, pH6.8 500 mL 166-27215
1/15mol/L Phosphate Buffer Solution, pH7.0 500 mL 163-27225
1/15mol/L Phosphate Buffer Solution, pH7.2 500 mL 160-27235
1/15mol/L Phosphate Buffer Solution, pH7.6 500 mL 167-27245
1/15mol/L Phosphate Buffer Solution, pH8.0 500 mL 164-27255

※ Mole notation shows the concentration as a phosphoric acid.



0.1mol/L Phosphate Buffer Solutions, Autoclaved

This is 0.1mol/L Phosphate Buffer Solutions prepared with sodium dihydrogenphosphate (NaH2PO4) and disodium hydrogenphosphate(Na2HPO4).
Autoclaved.

Product Name Package Size Wako Catalog No. Grade
0.1mol/L Phosphate Buffer Solution, pH6.0 500 mL 167-27125 for Biochemistry
0.1mol/L Phosphate Buffer Solution, pH6.4 500 mL 164-27135
0.1mol/L Phosphate Buffer Solution, pH6.8 500 mL 161-27145
0.1mol/L Phosphate Buffer Solution, pH7.0 500 mL 168-27155
0.1mol/L Phosphate Buffer Solution, pH7.2 500 mL 165-27165
0.1mol/L Phosphate Buffer Solution, pH7.6 500 mL 162-27175
0.1mol/L Phosphate Buffer Solution, pH8.0 500 mL 169-27185

※ Mole notation shows the concentration as a phosphoric acid.



1/15 mol/L Phosphate Buffer Powders

This is Buffer Powder prepared with disodium hydrogenphosphate(Na2HPO4) and potassium dihydrogen phosphate (KH2PO4).
Dissolving 1 package into water to make the total volume 1L, 1/15mol / L phosphate buffer solution with the predetermined pH value can be obtained .
Contains 20 packages.

Product Name Package Size Wako Catalog No. Grade
Phosphate Buffer Powder (1/15 mol/L, pH6.4) for 1 L x 20 166-14461 for Biochemistry
Phosphate Buffer Powder (1/15 mol/L, pH6.8) for 1 L x 20 163-14471
Phosphate Buffer Powder (1/15 mol/L, pH7.0) for 1 L x 20 161-12191
Phosphate Buffer Powder (1/15 mol/L, pH7.2) for 1 L x 20 160-14481
Phosphate Buffer Powder (1/15 mol/L, pH7.4) for 1 L x 20 167-14491

※ Mole notation shows the concentration as a phosphoric acid.



0.01mol/L Phosphate Buffered Saline Powder and Solution

Please use for the tissue-washing of immunohistochemical staining.

Product Name Package Size Wako Catalog No. Grade
Phosphate Buffered Saline Powder (0.01mol/L, pH7.2~7.4)
Composition (in 1 package): NaH2PO4…0.35g, Na2HPO4…1.28g, NaCl…8g
for 1 L x 20 162-19321 for Tissue Washing
0.01mol/L Phosphate Buffered Saline
Composition (in 1L): NaH2PO4・2H2O…0.45g, Na2HPO4・12H2O…3.23g, NaCl…8.0g, NaOH(pH adjustment), preservatives, pH7.2~7.4
* Contains preservatives. The use in living cells is not recommended.
1 L 164-18541 for Tissue Washing
20 L 162-18547


PBS(-) 【Phosphate Buffered Saline】

Phosphate Buffer Saline(PBS) of calcium and magnesium-free.

Product Name Abbreviation Package Size Wako Catalog No. Grade/Maker
1 x PBS(-)
Composition:137mmol/L NaCl, 8.1mmol/L Na2HPO4, 2.68mmol/L KCl, 1.47mmol/L KH2PO4, pH7.4
Sterile, attached cap with a cock
1 x PBS(-) 5 L 164-25511 for Biochemistry
Phosphate Buffer Saline 10 x (pH7.4) [PBS(-)]
Composition:1,370mmol/L NaCl, 81mmol/L Na2HPO4, 26.8mmol/L KCl, 14.7mmol/L KH2PO4
Autoclaved, DNase-free, RNase-free
10 x PBS(-) 500 mL 314-90185 Nippon Gene Co., Ltd.
PBS(-)
Composition (in 1L): KH2PO4…144mg, NaCl…9,000mg, Na2HPO4…421mg
Sterility tested, mycoplasma tested, endotoxin tested
1 x PBS(-) 500 mL 166-23555 for Cell Culture
10 x PBS(-)
Composition (in 1L): KH2PO4…1,440mg, NaCl…90g, Na2HPO4…4,210mg
Sterility tested, mycoplasma tested, endotoxin tested
10 x PBS(-) 500 mL 163-25265 for Cell Culture


D-PBS (-) Powder and Solutions

PBS (-) by Dulbecco's prescription. Calcium and magnesium-free.

Product Name Abbreviation Package Size Wako Catalog No. Grade Storage Condition
D-PBS(-), Powder
Endotoxin tested
1 x D-PBS(-) for 1 L x 10 293-72601 for Cell Culture Keep at 2-10℃.
for 10 L x 1 299-72603
D-PBS(-)
Sterility tested, mycoplasma tested, endotoxin tested
1 x D-PBS(-) 500 mL 045-29795 Keep below 25°C.
1 L 043-29791
10 x D-PBS(-)
Sterility tested, mycoplasma tested, endotoxin tested
10 x D-PBS(-) 500 mL 048-29805


PBS(-)-T

This is PBS (-) added Tween 20.

Product Name Abbreviation Package Size Wako Catalog No. Grade Storage Condition
1 x PBS(-)-T
Composition: 137mmol/L NaCl, 8.1mmol/L Na2HPO4, 2.68mmol/L KCl, 1.47mmol/L KH2PO4, pH7.4, 0.1w/v% Tween 20
Sterile, attached cap with a cock
1 x PBS(-)-T 5 L 161-25521 for Biochemistry Keep below 25°C.
PBS-T, pH7.4( x 10)
Composition: 1,370mmol/L NaCl, 81mmol/L Na2HPO4, 27mmol/L KCl,
15mmol/L KH2PO4, 1w/v% Tween 20
10 x PBS(-)-T 1 L 163-24361 for Blotting Keep at 2-10℃.


2. Tris buffer solutions

TBS 【Tris Buffered Saline】

This is tris buffered saline.

Product Name Abbreviation Package Size Wako Catalog No. Grade/Maker
1 x TBS
Composition: 137mmol/L NaCl, 2.68mmol/L KCl, 25mmol/L Tris-HCl, pH7.4
Sterile, attached cap with a cock
1 x TBS 5 L 209-19121 for Biochemistry
10 x TBS (pH7.4)
Composition: 1,370mmol/L NaCl, 26.8mmol/L KCl, 250mmol/L Tris
Autoclaved, DNase-free, RNase-free
10 x TBS 500 mL 317-90175 Nippon Gene Co., Ltd.
20 x TBS (pH7.4)
Composition: 400mmol/L Tris-HCl(pH 7.4), 3mol/L NaCl DNase-free, RNase-free
Note: The composition is different from Wako Catalog No. 317-90175 [10 x TBS] .
20 x TBS 500 mL 317-90371 Nippon Gene Co., Ltd.


TBS(-)-T

This is TBS added Tween 20.

Product Name Abbreviation Package Size Wako Catalog No. Grade Storage Condition
1 x TBS-T
Composition: 137mmol/L NaCl, 2.68mmol/L KCl, 25mmol/L Tris-HCl, pH7.4, 0.1w/v% Tween 20
Sterile, attached cap with a cock
1 x TBS-T 5 L 206-19131 for Biochemistry Keep below 25°C.
TBS-T, pH7.4( x 10)
Composition: 1,370mmol/L NaCl, 27mmol/L KCl, 250mmol/L Tris, 1w/v% Tween 20, 0.1v/v% ProClin150
10 x TBS-T 1 L 207-18061 for Blotting Keep at 2-10℃.


Tris-HCl 【Tris Hydrochloride Acid Buffer】

This is Tris-HCl buffer solution. Autoclaved, DNase-free, RNase-free.

Product Name Package Size Wako Catalog No. Manufacturer
1M Tris-HCl (pH7.0) 100 mL 311-90411 Nippon Gene Co., Ltd.
500 mL 313-90415
1M Tris-HCl (pH7.5) 100 mL 316-90221
500 mL 318-90225
1M Tris-HCl (pH8.0) 100 mL 312-90061
500 mL 314-90065
1M Tris-HCl (pH8.5) 100 mL 314-90401
500 mL 316-90405
1M Tris-HCl (pH8.8) 100 mL 311-90391
500 mL 313-90395
1M Tris-HCl (pH9.0) 100 mL 314-90381
500 mL 316-90385


TAE 【Tris-Acetate-EDTA】

Tris buffer solution containing acetic acid and EDTA.

Product Name Package Size Wako Catalog No. Grade/Manufacturer
1 x TAE
Composition: 20mmol/L Acetic Acid, 50mmol/L EDTA, 40mmol/L Tris, pH7.4
Sterile, attached cap with a cock
5 L 203-19141 for Genetic Research
10 x TAE
Composition: 400mmol/L Tris-acetate, 10mmol/L EDTA
5 L 318-90301 Nippon Gene Co., Ltd.
50 x TAE
Composition: 2mol/L Tris-acetate, 50mmol/L EDTA
500 mL 313-90035


TBE 【Tris-Borate-EDTA】

Tris buffer solution containing boric acid and EDTA.

Product Name Package Size Wako Catalog No.
(Manufacturer Code)
Manufacturer
5 x TBE
Composition: 0.445mol/L Tris-borate, 10mmol/L EDTA
1 L 318-90041 Nippon Gene Co., Ltd.
10 x TBE (pH8.3)
Composition: 0.89 mol/L Tris-borate, pH8.3, 20 mmol/L EDTA, DNase-free, RNase-free
1 L 344-07511
(MB04)
Dojindo Laboratories


TE 【Tris-EDTA】

Tris buffer solution containing EDTA.

Product Name Package Size Wako Catalog No. Manufacturer
TE (pH8.0)
Composition: 10mmol/L Tris-HCl(pH8.0), 1mmol/L EDTA(pH8.0)
Autoclaved, DNase free, RNase-free
100 mL 314-90021 Nippon Gene Co., Ltd.
500 mL 316-90025
100 mL x 6 310-90023


3. Related Products

Purified water

Product Name Package Size Wako Catalog No. Grade/Maker
Distilled Water 500 mL 043-16785 -
2 L 047-16783
2 L x 6 045-16784
5 L 041-16786
20 L 049-16787
CultureSure  Sterile Water (Endotoxin and Mycoplasma Free) 500 mL 039-24155 for Cell Culture
Sterile Water, Endotoxin Free 500 mL 196-15645
Distilled Water, Deionized, Sterile
Autoclaved, DNase free, RNase-free
100 mL 316-90101 Nippon Gene Co., Ltd.
100 mL x 6 312-90103
500 mL 318-90105
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