Wako Pure Chemical Industries, Ltd.Wako Pure Chemical Industries, Ltd. - Laboratory Chemicals
Home > Products > Life Science > LIF, human, recombinant, culture supernatant

LIF human, recombinant, culture supernatant for mouse ES cell culture

LIF (leukemia inhibitory factor) was found as a factor inhibiting proliferation of leukemic cells and inducing differentiation to macrophages. In addition, a variety of functions such as nerve differentiation, osteogenesis, lipid transportation of adipose cells and production of adrenal cortical hormones have been known.
LIF is also used in cell cultures to maintain undifferentiated state of ES cells because it shows inhibitory action on differentiation of mouse ES cells.
This product is a culture supernatant containing human LIF recombinant. It is suited to the culture of mouse ES cells and may also be applied to the culture of mouse iPS cells.


[INDEX]

1. Characteristics
2. Product Summary
3. Confirmation of Cell Differentiation Activity
4. Product List
5. Related Products

Package insert ( PDF 86.3 KB/1p)


1. Characteristics

- Suited to proliferation of mouse ES cells and maintenance of undifferentiated condition
- Low price

2. Product Summary
- Origin: BMT-10 cell-expressed human LIF
- Form: A culture supernatant containing D-MEM (high glucose) with L-glutamine and phenol red containing 10 % FBS.
- Sterilized by filtration through 0.2-μm filter
- Tested for mycoplasma
- Practical dilution factor: 1:1000
Each mL of this product is suited to preparation of 1 L of a medium for mouse ES cell culture.

3. Confirmation of cell differentiation activity
Confirmation of morphology of mouse ES cells during culture
  
  
This product (1:1000)
Product of
other company (1:1000)
LIF (-)
    Medium: G-MEM, 0.1 mmol/L 2-mercaptoethanol, 1 mmol/L sodium pyruvate,     0.1 mmol/L NEAA and 10% FBS
    Plate: Gelatin-coated, 12-well plate
    Number of cells: 300 cells/well
    Culture conditions: 37°C, 6 days
    Staining: Leishman staining

Confirmation of alkaline phosphatase (ALP) activity
  
     This product (1:1000) LIF (-)      
    Medium: D-MEM (high glucose), 0.1 mmol/L 2-mercaptoethanol, 0.1 mmol/L NEAA, 4 mmol/L glutamine, 15% FBS, 1x antibiotics
    Plate: Collagen Type I-coated, 12-well plate
    Number of cells: 7500 cells/well
    Culture conditions: 37°C, 4 days

The alkaline phosphatase activity, which is a marker for undifferentiation, was confirmed in mouse ES cells (D3 strain) cultured by the addition of this product using Fast Red substrate.
4. Product List
Product name Package Size Wako Catalog No. Grade
LIF, Human, recombinant, Culture Supernatant 1 mL 129-05601 for Cell Culture
1 mL x 10 125-05603
Not available for sale in Canada, the US and Korea.
QUOTATION REQUEST for reagents for ES cell culture   PAGE TOP

5. Related Products
StemSure LIF, mouse, recombinant, Solution
Product Name Package Size Wako Catalog No. Grade
StemSure LIF, Mouse, recombinant, Solution 1,000,000 units 199-16051 for Cell Culture

Liquid media
Product Name Package Size Wako Catalog No. Grade
D-MEM (High Glucose) with L-Glutamine and Phenol Red 500 mL 044-29765 for Cell Culture
1 L 042-29761
D-MEM (High Glucose) with L-Glutamine, Phenol Red, Sodium Pyruvate 500 mL 043-30085
D-MEM (High Glucose) with L-Glutamine, Phenol Red and HEPES 500 mL 048-30275
D-MEM (High Glucose) with Phenol Red 500 mL 045-30285
D-MEM without L-Glutamine and Phenol Red 500 mL 040-30095
D-MEM (Low Glucose) with L-Glutamine and Phenol Red 500 mL 041-29775
1L 049-29771
D-MEM/Ham's F-12 with Phenol Red, HEPES and Sodium Pyruvate 500 mL 042-30795
G-MEM with L-Glutamine and Phenol Red 500 mL 078-05525

Inhibitors
Product Name Package Size Wako Catalog No. Grade
Y-27632 1 mg 257-00511 for Cellbiology
5 mg 253-00513
Fibroblast Growth Factor(Basic), Human, recombinant [bFGF][FGF2] 50 μg 064-04541
100 μg 060-04543
6-Bromoindirubin-3'-oxime [BIO][GSK-3 Inhibitor IX] 1 mg 029-16241
LY-294002 5 mg 129-04861 for Biochemistry
10 mg 125-04863
25 mg 123-04864
PD-098059 5 mg 169-19211
PD184352 5 mg 169-25181 for Cellbiology
SB-203580 1 mg 190-11581 for Biochemistry
SU5402 1 mg 193-16071 for Cellbiology
Valproic Acid 5 g 227-01071 for Biochemistry
25 g 225-01072

QUOTATION REQUEST for reagents for ES cell culture  PAGE TOP
See the following leaflet for information on inhibitors for researches of stem cells Leaflet (742 KB/6p)


Products listed in this page are for research use only.  Do not administer each to human.
Home > Products > Life Science > LIF, human, recombinant, culture supernatant