LIF (leukemia inhibitory factor) was found as a factor inhibiting proliferation of leukemic cells and inducing differentiation to macrophages.
In addition, a variety of functions such as nerve differentiation, osteogenesis, lipid transportation of adipose cells and production of adrenal
cortical hormones have been known.
LIF is also used in cell cultures to maintain undifferentiated state of ES cells because it shows inhibitory action on differentiation of mouse ES cells.
This product is a culture supernatant containing human LIF recombinant. It is suited to the culture of mouse ES cells and may also be applied to the culture
of mouse iPS cells.
2. Product Summary
|- Suited to proliferation of mouse ES cells and maintenance of undifferentiated condition|
- Low price
3. Confirmation of cell differentiation activity
|- Origin: BMT-10 cell-expressed human LIF
- Form: A culture supernatant containing D-MEM (high glucose) with L-glutamine and phenol red containing 10 % FBS.
- Sterilized by filtration through 0.2-μm filter
- Tested for mycoplasma
- Practical dilution factor: 1:1000
Each mL of this product is suited to preparation of 1 L of a medium for mouse ES cell culture.
4. Product List
|Confirmation of morphology of mouse ES cells during culture|
Medium: G-MEM, 0.1 mmol/L 2-mercaptoethanol, 1 mmol/L sodium pyruvate, 0.1 mmol/L NEAA and 10% FBS
This product (1:1000)
other company (1:1000)
Plate: Gelatin-coated, 12-well plate
Number of cells: 300 cells/well
Culture conditions: 37°C, 6 days
Staining: Leishman staining
Confirmation of alkaline phosphatase (ALP) activity
Medium: D-MEM (high glucose), 0.1 mmol/L 2-mercaptoethanol, 0.1 mmol/L NEAA, 4 mmol/L glutamine, 15% FBS, 1x antibiotics
| || This product (1:1000)
Plate: Collagen Type I-coated, 12-well plate
Number of cells: 7500 cells/well
Culture conditions: 37°C, 4 days
The alkaline phosphatase activity, which is a marker for undifferentiation, was confirmed in mouse ES cells (D3 strain) cultured by the addition of
this product using Fast Red substrate.
Not available for sale in Canada, the US and Korea.
||Wako Catalog No.
|LIF, Human, recombinant, Culture Supernatant
||for Cell Culture
|1 mL x 10
5. Related Products
StemSure LIF, mouse, recombinant, Solution
See the following leaflet for information on inhibitors for researches
of stem cells Leaflet
Products listed in this page are for research use only. Do not administer
each to human.