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Mitochondrial DNA extraction kit from whole blood
mtDNA Extractor WB Kit


Recently there have been several reports that indicate relationship between diabetes mellitus or Alzheimer's disease and mitochondrial DNA (mtDNA) mutation, and mtDNA has been actively analyzed1), 2). 3). 4). However, in order to prepare highly-pure mtDNA, troublesome manipulation and a lot of time are needed, and it is not suitable for researches that aim to process numerous samples. Currently mtDNA analysis is generally conducted with a small amount of mtDNA that is included in total DNA extracted from cells with a mixture of a lot of genome DNA. The mtDNA Extractor WB Kit can quickly and easily extract highly-pure mtDNA from whole blood so that it is suitable for processing numerous samples.




1. Features



  • mtDNA can be extracted in a short time (about 90 minutes) with easy manipulation.

  • mtDNA can also be extracted from EDTA-treated blood or heparin-treated blood.

  • The extracted mtDNA is highly-pure and less contaminated with genome DNA and it is available for restriction enzyme treatment or DNA amplification reaction.

  • It is suitable for DNA amplification reaction treatment of numerous small- scale samples.

  • It does not use toxic solvent that corresponds to deleterious substance including phenol and chloroform.

2. Kit Contents


1. Leukocyte Isolation Solution 5.0 mL 1 vial
2. DNA Extraction Solution I 26.5 mL 1 vial
3. DNA Extraction Solution II (A) 1.3 mL 1 vial
4. DNA Extraction Solution II (B) 1.3 mL 1 vial
5. DNA Extraction Solution III 1.9 mL 1 vial
6. Sodium Iodide Solution 7.5 mL 1 vial
7. Washing Solution 50.0 mL 1 vial
mtDNA WB kit


3. Example of use


Electrophoresis of mtDNA extracted from whole blood by mtDNA Extractor WB Kit

An Example of use


4. Manipulation method


Manipulation method


5. References


  1. Kazuki Yasuda: Journal of Clinical and Experimental Medicine, 174, 420 (1995).
  2. Robert, E. D. et al.:Proc. Natl. Acad. Sci. USA, 94, 4526 (1997).
  3. Poulton, J. et al.:Prenatal Diagnosis, 16, 1247 (1996).
  4. Timothy, H. et al.:Proc. Natl. Acad. Sci. USA, 92, 6892 (1995).



6. Product List


Product Name
Pkg. Size
Wako Cat. No.
Grade
Storage Condition
    mtDNA Extractor WB Kit
25 tests
for Whole Blood mtDNA Extraction
Keep at 2-10°C

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