Wako Pure Chemical Industries, Ltd.Wako Pure Chemical Industries, Ltd. - Laboratory Chemicals

For establishment of high-expressing cells by CAG promoter
Transient vectors equipped with CAG promoter

pCAG Series

This products are gene expression vectors to be used in animal cells.
By the action of CAG promoter, in animal cells, the high expression of the target protein by gene transfer can be expected.


1. Features


  • High expression induction by CAG promoter
  • A line-up of four types of drug-resistant gene.
  • Optimizes the expression level of the drug-resistant gene.

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2. RFP expression analysis using pCAG vector

3. pCAG vector Product Outline

Vector size: described on each package insert
Purification method: Cesium chloride densitygradient centrifugation
Composition: 10 mmol/L Tris-HCl (pH 8.0), 1 mmol/L EDTA
Concentration: 1 μg/μL
Antibiotics for selecting Escherichia coli: described on the following product list
Antibiotics for selecting animal cells : described on the following product list
Nuclease contamination check: confirmed by electrophoresis
MCS promoter: CAG

4. pCAG vector Entrusted Services

pCAG vector



5. Product List

Storage Condition: Keep at -20°C.

Product Name
(Pkg. Insert)
Selection marker MCS
Restriction enzyme site
Pkg. Size Wako Cat. No. Full-length sequence
Escherichia coli Animal cells
pCAG-Neo Kanamycin G418 BamHI, EcoRV, KpnI, NotI, SalI, XhoI, AccI, HindIII, EcoRI, PstI, SmaI, SpeI 20 μg 163-25601 pCAG-Neo
Full-length sequence
pCAG-Hyg Hygromycin B BamHI, EcoRV, KpnI, NotI, SalI, XhoI, AccI, SpeI 20 μg 160-25611 pCAG-Hyg
Full-length sequence
pCAG-Bsd Blasticidin S EcoRV, KpnI, NotI, SalI, XhoI, AccI, HindIII, PstI, SpeI 20 μg 167-25621 pCAG-Bsd
Full-length sequence
pCAG-Ble Bleomycin BamHI, EcoRV, KpnI, NotI, SalI, XhoI, AccI, HindIII, PstI, SpeI 20 μg 164-25631 pCAG-Ble
Full-length sequence