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Laboratory Chemicals > Product Information> P > pEBMulti Series

Fast and Simple Generation of Cell Lines for Stable Gene Expression: New Additions to Our Vector Line-up
pEBMulti Series

pEBMulti vector is a gene expression vector for primates (human and monkey) and dog cell lines. pEBMulti vector is harboring OriP(replication origin) and EBNA-1 derived from Epstein-Barr Virus (EBV), therefore pEBMulti vector will be distributed to daughter cells by episomally-replicating system in vivo.
This vector can be used to generate cell lines for stable gene expression and to transfect multiple genes.

  1. pEBMulti Vector Map
  2. Product details
  3. pEBMulti Episomal distribution mechanism
  4. pEBMulti Vector data (complete sequence)
  5. Application
  6. References
  7. Products List

1. pEBMulti Vector Map

Wako Catalog No. 050-08121
Hygromycin B
Wako Catalog No. 057-08131
Wako Catalog No. 055-08311
Blasticidin S
Wako Catalog No. 052-08321
Wako Catalog No. 059-08331

2. Product details
Purification method: Cesium chloride density-gradient centrifugation
Formulation: 10 mmol/L Tris-HCl (pH 8.0),1 mmol/L EDTA
DNA Concentration: 1 μg/μL
Antibiotics for selecting E. coli and animal cells: Hygromycin B (Wako Cat. No. 050-08121) G418 or Geneticin R (Wako Cat. No. 057-08131)
Nuclease contamination check: Checked by electrophoresis
MCS Promoter: CAG
Storage Condition: -20°C

3. pEBMulti Episomal distribution mechanism

4. pEBMulti Vector data (complete sequence)
Wako Cat. No. Product Name Selection Marker of E. coli / Mammalian cells MCS restriction enzyme sites Complete sequence
050-08121 pEBMulti-Hyg Hygromycin B Cla I, EcoRV, Kpn I, Not I, Sal I, Xho I pEBMulti-Hyg complete sequence
057-08131 pEBMulti-Neo Kanamycin/G418 BamHI, Cla I, EcoRV, Kpn I, Not I,
Sal I, Xho I
pEBMulti-Neo complete sequence
055-08311 pEBMulti-Bsd Blasticidin S pEBMulti-Bsd complete sequence
052-08321 pEBMulti-Ble Bleomycin pEBMulti-Ble complete sequence
059-08331 pEBMulti-Puro Ampicillin/Puromycin BamHI, Not I, Sal I, Xho I pEBMulti-Puro complete sequence
↑ Click on each product Name to link for individual package inserts.

5. Application

Visit the page for more application data on pEBMulti-Hyg and pEBMulti-Neo.

Data provided by Dr. Yoshihiro Miwa and Dr. Junko Tanaka, Graduate School of Comprehensive Human Sciences, Tsukuba University

6. References
  1. Wako Jun-yaku Jiho, 79(3), 2 (2011)
    Miwa, Y. How to get the most out of the EBV-based vector pEBMulti! (written in Japanese)
  2. Wako Jun-yaku Jiho, 79(4), 12 (2011)
    Fukuda, M & Warashina, M. Generating cells lines for stable gene expression using pEBMulti vector (written in Japanese)
  3. "PCA-1/ALKBH3 contributes to pancreatic cancer by supporting apoptotic resistance and angiogenesis", Yamato I, Sho M, Shimada K, Hotta K, Ueda Y, Yasuda S, Shigi N, Konishi N, Tsujikawa K, Nakajima Y., Cancer Res. 2012 Sep 15;72(18):4829-39.
  4. "CpG site-specific alteration of hydroxymethylcytosine to methylcytosine beyond DNA replication", Kubosaki A, Tomaru Y, Furuhata E, Suzuki T, Shin JW, Simon C, Ando Y, Hasegawa R, Hayashizaki Y, Suzuki H., Biochem Biophys Res Commun. 2012 Sep 14;426(1): 141-7.

7. Products List
Product Name Package Size Wako Catalog No. Storage Condition
pEBMulti-Hyg 20 μg 050-08121 Keep at -20°C
pEBMulti-Neo 20 μg 057-08131
pEBMulti-Bsd 20 μg 055-08311
pEBMulti-Ble  20 μg 052-08321
pEBMulti-Puro 20 μg 059-08331

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Laboratory Chemicals > Product Information> P > pEBMulti Series

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