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1-week establishment of stable cell lines
pEBMulti Vector

Leaflet (1.8 MB/2 p)
⇒ a compressed file of sequence information


pEBMulti vector is a gene expression vector for primates (human and monkey) and dog cell lines.
pEBMulti vector is harboring OriP(replication origin) and EBNA-1 derived from Epstein-Barr Virus (EBV), therefore pEBMulti vector will be distributed to daughter cells by episomally-replicating system in vivo.

Features
  1. Easy!!! 1 week-establishment of Stable Cell Lines
  2. Not integrated into Host Cell Genome
  3. Applicable as a Shuttle Vector *

  4. *: pEBMulti is used as a shuttle vector for clone selection of E. coli and mammalian cells by single antibiotic reagent.
pEBMulti-Hyg (11,0004 bp)pEBMulti-Hyg (11,0004 bp)
RFP expressing stable cell suspension after 8 days of Hygromycin B selection


  INDEX
  1. Multiple cloning site (MCS)
  2. Comparison with conventional vector
  3. Product Information
  4. OriP & EBNA1
  5. Population analysis of RFP expressed cells
  6. Effect of pEMulti vector
  7. Product List



1. Multi Cloning Site (MCS) Each MCS sequencing primer is shown on each package insert.
Multiple cloning site of pEBMulti-Hyg and pEBMulti-Neo


2. Comparison with conventional vector
3. Product Information
  • Vector Size:
     Approx. 11 kbp (pEBMulti-Hyg)
     Approx. 10 kbp (pEBMulti-Neo)
  • Purification Methods:
     Cesium chloride density-gradient centrifugation
  • Formulation:
     10 mmol/L Tris-HCl (pH 8.0), 1 mmol/L EDTA
  • Concentration: 1 μg/μL
  • Selection Markers:
     Hygromycin B (Wako Cat. No. 050-08121)
     G418 or Geneticin® (Wako Cat. No. 057-08131)
  • Nuclease Contamination Check: Electrophoresis
  • MCS promoter: CAG
4. OriP & EBNA1






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5. Population analysis of RFP expressed cells

pEBMlti-RFP ⇒ Transfection into HEp-2 cells ⇒ Drug Screening for 4 days ⇒ FCM EBNA1 gene of pEBMluti is contributed to easily establish higher expression of stable cell line


6. Effect of pEBMulti vector Effect of pEBMulti vector

Shorten term for establishment of stable cell line
Shorten term for establishment of stable cell line
Maintain Luciferase gene expression
(7 days)
Maintein Luciferase gene expression (7 days) without subculture
Maintain Luciferase gene expression
(16 days)
Maintain Luciferase gene expression (16 days) after subculture
7. Product List
Product Name Package Size Wako Catalog No. Grade Storage
pEBMulti-Hyg   (1 μg/μL)
20 μg
050-08121
for Genetic Research
Keep at -20°C
pEBMulti-Neo   (1 μg/μL)
20 μg
057-08131



Related Products
Product Name Package Size Wako Catalog No. Grade Storage
pEBMulti-Bsd   (1 μg/μL)
20 μg
055-08311
for Genetic Research
Keep at -20°C
pEBMulti-Ble     (1 μg/μL)
20 μg
052-08321
pEBMulti-Puro   (1 μg/μL)
20 μg
059-08331
(Note)    Listed products are for research use only. Do not administer each to human.

 

 
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