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Lewy Body Marker
Labeled Phosphorylated α-Synuclein Antibodies (pSyn#64)

pSyn#64 α-Synuclein is a major constituent protein of Lewy bodies (LBs), which are the biomarkers for Parkinson's Disease (PD) and Dementia with Lewy bodies (DLB). About 90% α-Synuclein deposited in LBs is phosphorylated at Ser129. Anti Phosphorylated α-Synuclein Monoclonal Antibody (pSyn#64)(Wako Cat. No. 015-25191) is a mouse antibody which specifically reacts with α-Synuclein phosphorylated at Ser129, and is applicable to immunohistochemical and biological studies related to PD and LB pathology. This is the same antibody labeled with Biotin or FITC.


1. LINK

2. Features

  • The secondary antibody process could be eliminated
  • Reduced background
  • Evaluate by immunohistochemistry test

3. Applications -Immunohistostaining-

Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), Biotin-conjugated

Biotin-conjugated Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), Biotin-conjugated (Wako Cat. No.010-26481)
biotin-cojungated Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64)
(Wako Cat. No.: 015-25191)
     +
Secondary antibody Biotin-conjugated anti Mouse IgG
→ The image using the conjugated antibody (upper) shows clearer localization signals of aggregated α-Synuclein than using the secondary antibody (bottom)
■Assay condition
  • Sample: Striatum of 6-week wild-type mouse injected aggregated α-synuclein
  • Section: 50 μm-vibratome sections
  • Antibody concentration: 1 : 1,000
  • Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), FITC-conjugated

    FITC-conjugated Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), FITC-conjugated (Wako Cat. No.017-26491)
    FITC-conjugated Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64)
    (Wako Cat. No.: 015-25191)
         +
    Alexa Fluor® 488-conjugated secondary antibody

    Green: Phosphorylated α-Synuclein

    Blue: DAPI (nuclei)

    → The image using the conjugated antibody (upper) shows similar localization signals of aggregated α-Synuclein to using the secondary antibody (bottom).

    ■Assay condition
  • Sample: Aggregate of α-Synuclein in the stable
    α-Synuclein expressing SH-SY5Y neuroblastoma cell lines after transfection with C-terminally truncated α-Synuclein mutant (AA 1-122).
  • Antibody concentration: 1 : 1,000

  • All immunohistochemistry data was provided by Kuwabara T.and Iwatsubo T., Department of Neuropathology, Graduate School of Medicine, The University of Tokyo, Japan

    4. Antibody Information

     

    Anti Phosphorylated α-Synuclein,

    Monoclonal Antibody (pSyn#64), Biotin-conjugated

    Anti Phosphorylated α-Synuclein,

    Monoclonal Antibody (pSyn#64), FITC-conjugated

    Conjugate Biotin FITC
    Antigen 124-134 amino acid of human αSynuclein with phosphorylated at Ser129
    Formulation PBS with 0.05% Sodium Azide
    Subclass Mouse IgG
    Reactivity human, mouse and rat
    Application Immunohistochemistry ( 1:200 - 5,000 )

    5. Product List

    Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
    Anti Phosphorylated α-Synuclein,
    Monoclonal Antibody (pSyn#64), Biotin-conjugated
    100 μL 010-26481 for Immunochemistry Keep at 2~10°C.
    Anti Phosphorylated α-Synuclein,
    Monoclonal Antibody (pSyn#64), FITC-conjugated
    100 μL 017-26491

    6. Related Products

    Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
    Anti Phosphorylated α-Synuclein, Monoclonal Antibody
    (pSyn#64)
    50 μL 015-25191 for Immunochemistry Keep at -20°C.
    α-Synuclein, Human, recombinant 0.5 mg 190-17941 for Cellbiology
    β-Synuclein, Human, recombinant 0.5 mg 197-17951
    γ-Synuclein, Human, recombinant 0.5 mg 194-17961
    Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
    Anti Iba1, Rabbit (for Immunocytochemistry) ⇒ details 50 μg 019-19741 for Immunochemistry Keep at -20°C.
    Anti Iba1, Rabbit (for Western Blotting) ⇒ details 50 μg 016-20001
    Anti Iba1, Rabbit, Biotin-conjugateddetails 100 μL 016-26461 Keep at 2-10°C.
    Anti Iba1, Rabbit, Red Fluorechrome (635)-conjugated
    details
    100 μL 013-26471
    Anti Iba1, Monoclonal Antibody (NCNP24) ⇒ details 10 μg 012-26723 Keep at -20°C.
    50 μg 016-26721
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