Wako Pure Chemical Industries, Ltd.Wako Pure Chemical Industries, Ltd. - Laboratory Chemicals
Home > Products > Life Science > StemSure Series for culture of ES cells

StemSure Series for ES · iPS Cell Culture

StemSure Series is a group of products whose quality has been confirmed by culturing mouse ES D3 strain cells and performing a colony forming test and alkaline phosphatase staining in addition to the quality specifications for liquid media for general uses.

Please browse the brochure:

[INDEX]

1. Product Line-Up

2. Product List

3. LINK

1. Product Line-up

Product Name Package Size LINK
StemSure hPSC Medium Δ A serum-free and feeder-free medium
for human pluripotent stem cell (PSC) culture.
 
StemSure D-MEM (High Glucose)
with Phenol Red and Sodium Pyruvate
An optimized medium for the culture of mouse ES cells. ⇒ LINK
StemSure Serum Replacement (SSR) This serum-free medium is particularly suited for the stable growth
of ES and iPS cells. SSR is not designated as a poison.
⇒ LINK
StemSure 10 mmol/L 2-Mercaptoethanol Solution
( x 100)
2ME is a reducing agent necessary for culturing ES and iPS cells.  
StemSure 50 mmol/L Monothioglycerol Solution
( x 100)
MTG is a reducing agent necessary for culturing ES and iPS cells,
and can be used equally with 2ME. MTG is not designated as a poison.
⇒ LINK
StemSure 0.1w/v% Gelatin Solution
(⇒ Not available for sales in the US.)
Gelatin Soln. used for coating cell culture containers. ⇒ LINK
StemSure LIF, mouse, recombinant,
solution for mouse ES cell culture
LIF is a factor maintaining undifferentiating property of mouse ES cells
in the culture.
⇒ LINK
StemSure Freezing Medium
(⇒ Not available for sales in the US.)
Serum-free Cell Freezing Medium suitable to preservation of ES cells
and various animal cells.
⇒ LINK

⇒ Quality Test


1-(1) StemSure 50 mmol/L Monothioglycerol Solution (x100)

Monothioglycerol (MTG) can be used as an alternative for 2-mercaptoethanol (2ME) since it exhibits comparable performance to 2ME when it is added to a medium in the culture of mouse ES D3 strain cells.  Although 2ME is an essential factor for the culture of ES cells, it was designated to a poison by the legal revision in 2008 and media containing 2ME and their waste solutions are all handled as poisons because there is no exclusion rule by concentration.  On the other hand, MTG has not been designated to a poison and media and waste solutions containing MTG can be handled as general substances.

[Quality tests]: Colony formation, alkaline phosphatase staining, sterility test, external appearance and mycoplasma test

 <<Cell proliferation>>

StremSureTM Cell proliferation

<<Colony formation>>

StremSureTM Colonyformation
<Composition of the medium>
Add one of the following ingredients (i) to (iv) to StemSure D-MEM + 15% KSR +
4 mmol/L L-Glutamine + 2 x Non-essential Amino Acids + 1 x Penicillin-Streptomycin + 1000 U LIF.
(i) 0.1 mmol/L 2-mercaptoethanol (2ME)
(ii) 0.1 mmol/L monothioglycerol (MTG)
(iii) 0.5 mmol/L monothioglycerol (MTG)
(iv) No addition

<Number of cells seeded)
3400 cells/cm2
(Use collagen-coated 12-well plates.)

<Composition of the medium>
Add one of the following ingredients (i) to (iv) to StemSure D-MEM + 15% KSR + 4 mmol/L L-Glutamine + 2 x Non-essential Amino Acids + 1 x Penicillin-Streptomycin + 1000 U LIF.
(i) 0.1 mmol/L 2-mercaptoethanol (2ME)
(ii) 0.1 mmol/L monothioglycerol (MTG)
(iii) 0.5 mmol/L monothioglycerol (MTG)
(iv) No addition

<Number of cells seeded)
1000 cells/cm2
(Use collagen-coated 6-well plates.)

<Culture period>
10 days


<<Generation of undiffrrentiated markers>>


<Composition of the medium>
Add one of the following ingredients (i) to (v) to StemSure D-MEM + 15% KSR + 4 mmol/L L-Glutamine + 2 x Non-essential Amino Acids + 1 x Penicillin-Streptomycin.
(i) 0.1 mmol/L 2-mercaptoethanol + 1000 U LIF
(ii) 0.1 mmol/L monothioglycerol + 1000 U LIF
(iii) 0.5 mmol/L monothioglycerol + 1000 U LIF
(iv) 0.1 mmol/L 2-mercaptoethanol + 50 ng/mL Wnt-3a
(v) 0.1 mmol/L 2-mercaptoethanol

<Number of cells seeded)
3400 cells/cm2
(Use collagen-coated plates.)

<Culture period>
5 days



1-(2) StemSure 0.1 w/v% gelatin solution

This product is used for coating cell culture containers. It is adjusted to the concentration of use and can be used as it is. It has been confirmed that mouse ES D3 strain cells can be cultured in a plate coated with this product while maintaining the undifferentiated condition. This product has been prepared using gelatin derived from porcine skin.

[Quality tests]: Colony formation, alkaline phosphatase staining, sterility test, external appearance, bacterial endotoxins test and mycoplasma test
<<Colony formation>>
StremSureTM Colonyformation


<Composition of the medium>
D-MEM + 15% KSR + 4 mmol/L L-Glutamine + 2 x Non-essential Amino Acids + 1 x Penicillin-Streptomycin + 0.1 mmol/L StemSure 2-Mercaptoethanol + 1000 U LIF

<Number of cells seeded>
500 cells/well (use gelatin-coated 6-well plates)

<Culture period>
10 days




2. Product List

Product Name Package Size Wako Catalog No. Grade
StemSure hPSC Medium Δ 100 mL 197-17571 for Cell Culture
100 mL x 4 193-17573
StemSure D-MEM (High Glucose) with Phenol Red and Sodium Pyruvate 500 mL 197-16275
StemSure Serum Replacement (SSR) 500 mL 197-16775
StemSure 10 mmol/L 2-Mercaptoethanol Solution ( x 100) 100 mL 198-15781
StemSure 50 mmol/L Monothioglycerol Solution ( x 100) 100 mL 195-15791
StemSure 0.1w/v% Gelatin Solution    (⇒ Not available for sales in the US.) 500 mL 190-15805
StemSure LIF, mouse, recombinant, solution for mouse ES cell culture 1,000,000 units 199-16051
1,000,000 units x 10 195-16053
StemSure Freezing Medium   (⇒ Not available for sales in the US.) 100 mL 195-16031
Products listed in this page are for research use only.   Do not administer each to human.



3. LINK


Note
  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
  • Please visit our online catalog to search for other products from Wako: http://www.e-reagent.com
  • This information may contain products that cannot be exported to your country due to regulations.
  • Bulk quote requests for some products are welcomed. Please contact us or our distributor near you.
Home > Products > Life Science > StemSure Series for culture of ES cells